Mouse CD133 Antibody

Catalog # Availability Size / Price Qty
MAB11332-100
MAB11332-SP
Detection  of CD133 in D3 Mouse Cells by Flow Cytometry.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Mouse CD133 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD133 in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 217106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD133
Accession # O54990
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection  of CD133 in D3 Mouse Cells by Flow Cytometry. View Larger

Detection of CD133 in D3 Mouse Cells by Flow Cytometry. D3 mouse cells were stained with Rat Anti-Mouse CD133 Monoclonal Antibody (Catalog # MAB11332, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry CD133  in Mouse Kidney. View Larger

CD133 in Mouse Kidney. CD133 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD133 Monoclonal Antibody (Catalog # MAB11332) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in epithelial cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD133

Mouse Prominin-1 (also known as CD133) is a 867 aminoacids glycoprotein encoded by the PROM1 gene. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM. In human, Prom-1 is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. In Mouse, at least 7 different isoforms have been proposed. Human and mouse PROM-1 share only 61% aminoacid sequence identity.

Entrez Gene IDs
8842 (Human); 19126 (Mouse)
Alternate Names
AC133 Stargardt disease 4 (autosomal dominant); AC133; CD133 retinal 2; CD133; PROM1; prominin (mouse)-like 1; Prominin 1; PROML1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Rat IgG2B Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse CD133 Antibody

There are currently no reviews for this product. Be the first to review Mouse CD133 Antibody and earn rewards!

Have you used Mouse CD133 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review