Mouse CD40 Ligand/TNFSF5 Biotinylated Antibody Summary
Accession # P27548
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CD40 Ligand/TNFSF5 in Mouse Splenocytes. CD40 Ligand/TNFSF5 was detected in immersion fixed mouse splenocytes stimulated with PHA (left panel) or untreated (right panel) using Goat Anti-Mouse CD40 Ligand/TNFSF5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1163) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD40 Ligand/TNFSF5
CD40 ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 260 amino acid (aa) type II transmembrane glycoprotein belonging to the TNF family. Murine CD40L consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane domain, and 214 aa extracellular domain bearing a single glycosylation site (1, 2). CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including NK cells, mast cells, basophils and eosinophils. Murine CD40L shares 78% amino acid sequence identity with human CD40L. Native bioactive soluble CD40L exists. Soluble human trimeric CD40L secreted by stimulated T cells has been shown to be generated by proteolysis in the microsomes (3). Both membrane bound and soluble CD40L induce similar effects on B cells (3, 4). The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells, and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the soluble trimeric form of CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members (2). The genetic defect in the hyper-IgM syndrome is due to point mutations or deletions of the gene encoding the CD40L, which prevent CD40L from interacting with CD40 (5-7). CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into the cell cycle, isotype switching, Ig secretion, and memory generation (8, 9). CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation (10).
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- Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
- Fabienne, P. et al. (1996) J. Biol. Chem. 271:5965.
- Fabienne, P. et al. (1996) Eur. J. Immunol 26:725.
- Arrufo, A. et al. (1993) Cell 72:291.
- Hill, A. and N. Chapel et al. (1993) Nature 361:494.
- Korthauer, U. et al. (1993) Nature 361:539.
- Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543.
- Fanslow, W.C. et al. (1994) Seminars in Immunology 6:267.
- Kooten, C.V. and J. Banchereau (2000) J. Leukoc. Biol. 67:2.
Citation for Mouse CD40 Ligand/TNFSF5 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Reduced pathology following infection with transgenic Leishmania major expressing murine CD40 ligand.
Authors: Field AE, Wagage S, Conrad SM
Infect. Immun., 2007;75(6):3140-9.
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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