Detection of Mouse CD90/Thy1 by Western Blot.
Western blot shows lysates of mouse thymus tissue, mouse brain tissue, EL‑4 mouse lymphoblast cell line, and CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7335) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD90/Thy1 at approximately 25-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD90, also known as Thy1 (THYmocyte differentiation antigen 1) is a GPI-linked glycoprotein expressed by
endothelial cells, hematopoietic stem cells, and in developing nervous
tissue. Unlike human CD90, mouse CD90 is also highly expressed on T
cells and is involved in T cell activation. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
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