Mouse CD90/Thy1 Antibody

Name: Mouse CD90/Thy1 Antibody
Brand: R&D Systems
SKU: AF7335
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF7335

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all CD90/Thy1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD90/Thy1
Gln20-Lys130
Accession # P01831

Specificity

Detects mouse CD90/Thy1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human CD90/Thy1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse CD90/Thy1 Antibody

Detection of Mouse CD90/Thy1 by Western Blot.

Western blot shows lysates of mouse thymus tissue, mouse brain tissue, EL-4 mouse lymphoblast cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7335) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD90/Thy1 at approximately 25-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse CD90/Thy1 Antibody

Application

Recommended Usage

Western Blot

0.2 µg/mL
Sample: Mouse thymus tissue, mouse brain tissue, EL‑4 mouse lymphoblast cell line, and CTLL‑2 mouse cytotoxic T cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD90/Thy1

CD90, also known as Thy1 (THYmocyte differentiation antigen 1) is a GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, and in developing nervous tissue. Unlike human CD90, mouse CD90 is also highly expressed on T cells and is involved in T cell activation. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.