Mouse CEACAM-1/CD66a Antibody

Catalog # Availability Size / Price Qty
AF6480
AF6480-SP
Detection of Mouse CEACAM‑1/CD66a by Western Blot.
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Citations (1)
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Mouse CEACAM-1/CD66a Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CEACAM-1/CD66a in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CEACAM-1/CD66a
Met1-Gly428
Accession # P31809
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse CEACAM‑1/CD66a by Western Blot. View Larger

Detection of Mouse CEACAM‑1/CD66a by Western Blot. Western blot shows lysates of mouse liver tissue and mouse small intestine tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CEACAM‑1/CD66a between approximately 110 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CEACAM-1 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CEACAM-1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

Immunohistochemistry CEACAM‑1/CD66a in Mouse Liver. View Larger

CEACAM‑1/CD66a in Mouse Liver. CEACAM‑1/CD66a was detected in perfusion fixed frozen sections of mouse liver using Sheep Anti-Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-1/CD66a

CEACAM-1 (Carcinoembryonic antigen-related cell adhesion molecule 1; also BGP-1, CD66a and MHVR1) is a 110-120 kDa member of the CEACAM subfamily, CEA family of proteins. It has a wide expression pattern, being found on neutrophils, dendritic cells, endothelial cells, colonic epithelium and hepatocytes. It mediates cell adhesion, and appears to regulate insulin levels and signaling by interacting with the insulin receptor. It also demonstrates proangiogenic effects by inducing endothelial cells to proliferate and form capillary-like tubules. Finally, CEACAM-1 is a known receptor for mouse hepatitis virus. Mature mouse CEACAM-1 is a 487 amino acid (aa) type I transmembrane glycoprotein. Its contains a 394 aa extracellular region (aa 35-428) that shows one V-type (aa 35-142) and three C2-type
(aa 147-411) Ig-like domains, plus a 74 aa cytoplasmic domain. Three alternate splice forms exist. One contains a four aa substitution for aa 455-521, a second shows a Gln substitution for aa 142-322, and a third possesses a combination of the first two splice patterns. CEACAM-1 forms homodimers. Over aa 35-428, mouse CEACAM-1 shares 56% and 70% aa identity with human and rat CEACAM-1, respectively.

Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 1
Entrez Gene IDs
634 (Human); 26365 (Mouse)
Alternate Names
antigen CD66; BGP1; BGP-1; BGP1BGPBiliary glycoprotein 1; BGPI; Biliary Glycoprotein 1; biliary glycoprotein adhesion molecule; carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein); carcinoembryonic antigen-related cell adhesion molecule 1; CD66a antigen; CD66a; Cea-1; CEACAM1; CEACAM-1; Hv-1; Hv-2; MHVR

Product Datasheets

Citation for Mouse CEACAM-1/CD66a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet
    Authors: A Schmidt, TV Woedtke, J Stenzel, T Lindner, S Polei, B Vollmar, S Bekeschus
    Int J Mol Sci, 2017;18(4):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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