Mouse Complement Factor D/Adipsin DuoSet ELISA

  (1 citations)     
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    • Assay Type
      Solid Phase Sandwich ELISA
    • Format
      96-well strip plate
    • Sample Volume Required
      100 µL
    • Sufficient Materials
      For five 96-well plates*
    • Specificity
      Please see the product datasheet
    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
    Ancillary Reagent Kit Available
    DY008 , DuoSet ELISA Ancillary Reagent Kit 2 - Please Inquire

    This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.


    Product Features
    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
    • Development protocols are provided to guide further assay optimization
    • Assay can be customized to your specific needs
    • Economical alternative to complete kits
    Kit Content
    • Capture Antibody
    • Detection Antibody
    • Recombinant Standard
    • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
    Other Reagents Required

    PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

    Wash Buffer: (Catalog # WA126), or equivalent

    Reagent Diluent*

    Blocking Buffer*

    Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

    Stop Solution: 2 N H2SO4 (Catalog # DY994)

    Microplates: R&D Systems (Catalog # DY990), or equivalent

    Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

    *For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

    Product Datasheets

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Certificate of Analysis Lookup

    Certificate of Analysis Request Form

    To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Preparation and Storage
    • Stability & Storage
      Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    Background: Complement Factor D/Adipsin
    Complement Factor D, also known as adipsin, is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3.3 Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors.
    • Entrez Gene IDs:
      1675 (Human); 11537 (Mouse); 54249 (Rat)
    • Alternate Names:
      Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 3.4.21.46; PFD; Properdin factor DADIPSIN
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes
    Authors: M Rödiger, MW Werno, I Wilhelmi, C Baumeier, D Hesse, N Wettschure, S Offermanns, K Song, M Krau beta, A Schürmann
    Mol Metab, 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates

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