Mouse DcTRAILR1/TNFRSF23 Antibody (270012)
R&D Systems | Catalog # MAB2378
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 270012
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DcTRAIL R1/TNFRSF23
Met30-Ser156
Accession # Q9ER63
Met30-Ser156
Accession # Q9ER63
Specificity
Detects mouse DcTRAIL R1/TNFRSF23 in direct ELISAs and Western blots. In Western blots, less than 10% cross-reactivity with recombinant mouse (rm) 4-1BB Ligand, recombinant human (rh) BAFF R, rhDcTRAIL R3, rmFas, rmOPG, and rhTRAIL R4 is observed and no cross‑reactivity with rmCD27, rmCD30, rmDR3, rhDR6, rmEDAR, recombinant feline Fas, rhFas, recombinant rat Fas, rmGITR, rmHVEM, rmLymphotoxin beta R, rmNGF R, rmOX40, rmTNF RI, rmTNF RII, rhTRAIL R1, or rhTROY is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse DcTRAILR1/TNFRSF23 Antibody (270012)
DcTRAIL R1/TNFRSF23 in Mouse Splenocytes.
DcTRAIL R1/TNFRSF23 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse DcTRAIL R1/TNFRSF23 Mono-clonal Antibody (Catalog # MAB2378) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse DcTRAILR1/TNFRSF23 Antibody (270012)
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Western Blot
1 µg/mL
Sample: Recombinant Mouse DcTRAIL R1/TNFRSF23 under non-reducing conditions only
Sample: Recombinant Mouse DcTRAIL R1/TNFRSF23 under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DcTRAILR1/TNFRSF23
Long Name
Decoy TRAIL Receptor 1
Alternate Names
DcTRAIL R1, SOB, TNFRH1, TNFRSF23
Entrez Gene IDs
79201 (Mouse)
Gene Symbol
TNFRSF23
UniProt
Additional DcTRAILR1/TNFRSF23 Products
Product Documents for Mouse DcTRAILR1/TNFRSF23 Antibody (270012)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse DcTRAILR1/TNFRSF23 Antibody (270012)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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