Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Neutralization, Immunocytochemistry, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Dectin-1/CLEC7A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dectin-1/CLEC7A
Phe69-Leu244
Accession # Q6QLQ4

Specificity

Detects mouse Dectin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Dectin-1 and recombinant mouse Dectin-2 alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Dectin‑1/CLEC7A Antibody

Detection of Yeast Dectin-1/CLEC7A by Immunocytochemistry/Immunofluorescence

Detection of Yeast Dectin-1/CLEC7A by Immunocytochemistry/Immunofluorescence

Binding of cHis-sDectin-1 to Saccharomyces cerevisiae cells.Purified cHis-sDectin-1 was diluted to a concentration of 25 µg/ml in blocking buffer with 500 µg/ml, 100 µg/ml or no laminarin. This was added to Saccharomyces cerevisiae yeast cells from overnight culture in blocking buffer (PBS with 3% FBS). The cells were then probed with an mDectin-1 goat polyclonal antibody (1∶200; AF1756; R&D Systems) and AlexaFluor546-conjugated anti-goat antibody (1∶100; Catalog Number A-11056; Molecular Probes). After which, the cells were washed with PBS and fixed using 4% paraformaldehyde. The cells were then resuspended in PBS and visualized by phase contrast and fluorescent microscopy at (A) 200× magnification and (B) 600× magnification. Images are cropped or scaled to fit the illustration. cHis-sDectin-1 stained yeast cells was also analyzed by flow cytometry (C). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0052785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Yeast Dectin-1/CLEC7A by Immunocytochemistry/Immunofluorescence

Detection of Yeast Dectin-1/CLEC7A by Immunocytochemistry/Immunofluorescence

Binding of cHis-sDectin-1 to Saccharomyces cerevisiae cells.Purified cHis-sDectin-1 was diluted to a concentration of 25 µg/ml in blocking buffer with 500 µg/ml, 100 µg/ml or no laminarin. This was added to Saccharomyces cerevisiae yeast cells from overnight culture in blocking buffer (PBS with 3% FBS). The cells were then probed with an mDectin-1 goat polyclonal antibody (1∶200; AF1756; R&D Systems) and AlexaFluor546-conjugated anti-goat antibody (1∶100; Catalog Number A-11056; Molecular Probes). After which, the cells were washed with PBS and fixed using 4% paraformaldehyde. The cells were then resuspended in PBS and visualized by phase contrast and fluorescent microscopy at (A) 200× magnification and (B) 600× magnification. Images are cropped or scaled to fit the illustration. cHis-sDectin-1 stained yeast cells was also analyzed by flow cytometry (C). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0052785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Yeast Dectin-1/CLEC7A by Immunocytochemistry/Immunofluorescence

Detection of Yeast Dectin-1/CLEC7A by Immunocytochemistry/Immunofluorescence

Binding of cHis-sDectin-1 to Saccharomyces cerevisiae cells.Purified cHis-sDectin-1 was diluted to a concentration of 25 µg/ml in blocking buffer with 500 µg/ml, 100 µg/ml or no laminarin. This was added to Saccharomyces cerevisiae yeast cells from overnight culture in blocking buffer (PBS with 3% FBS). The cells were then probed with an mDectin-1 goat polyclonal antibody (1∶200; AF1756; R&D Systems) and AlexaFluor546-conjugated anti-goat antibody (1∶100; Catalog Number A-11056; Molecular Probes). After which, the cells were washed with PBS and fixed using 4% paraformaldehyde. The cells were then resuspended in PBS and visualized by phase contrast and fluorescent microscopy at (A) 200× magnification and (B) 600× magnification. Images are cropped or scaled to fit the illustration. cHis-sDectin-1 stained yeast cells was also analyzed by flow cytometry (C). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0052785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Dectin-1/CLEC7A by Western Blot

Detection of Mouse Dectin-1/CLEC7A by Western Blot

MTX amplification and characterization of sDectin-1 cell pools.(A) Western blotting of supernatant samples from cultures adapted to different MTX concentrations using an mDectin-1 goat polyclonal antibody (1∶1000; AF1756; R&D Systems) with a HRP conjugated anti-goat antibody (1∶2000; Catalog number V8051; Promega). cHis pool and nHis pool are cell pools producing sDectin-1 with histidine tagged at the C- and N-terminals respectively. (B) Shake flask batch culture cell growth and sDectin-1 production profiles of sDectin-1 producing cell pools. Values shown represent mean values obtained from three replicate flasks. Error bars indicate the standard deviation of the experiment. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0052785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse Dectin-1/CLEC7A Antibody by Western Blot

Detection of Mouse Mouse Dectin-1/CLEC7A Antibody by Western Blot

OatN beta -glucan binds to HPCA, promotes microglia up-take of oatN and inhibits dectin-1 activation, whereas OatN digalactosyldiacylglycerol (DGDG) inhibits oatN beta -glucan access to dectin-1. H) A representative western blot of p-Syk in BV2 cells treated with Eth (200 mm) or Eth plus OBG (20 mg mL−1). Results represent 1 out of 3 independent experiments. Band intensity was quantified and normalized to beta -actin and the results are presented between the panels. I) The surface plasmon resonance (SPR) assay was performed to determine OBG binding to dectin-1. (Upper panel) BG (commercial beta -glucan, 100 ng mL−1), PBLP (liposomes made by peripheral blood cell derived lipids), OatN (20 mg mL−1), and OBG (20 mg mL−1) were used as analytes. (Bottom panel) OBG-DGDG (OBG remove DGDG, 20 or 60 mg mL−1) and OBG-PC (OBG remove PC, 20 mg mL−1) were used as analytes. Results represent 1 out of 3 independent experiments. J) Dectin-1 binding to HPCA-Rab11a complex was detected by immunoprecipitation. HPCA complex in BV2 cells treated with Eth or OBG was pulled down by anti-HPCA antibody (1 mg mL−1). Dectin-1 and Rab11a were probed in a western blot. Results represent 1 out of 3 independent experiments. Band intensity was quantified and normalized by HPCA, and the results are presented between the panels. K) Confocal images of dectin-1 (green) overlay with Rab11a (red) in BV2 cells treated with Eth (200 mm) or Eth plus OBG (20 mg mL−1). Results represent 1 out of 3 independent experiments. Five random fields were photographed, and representative results are shown. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34897972), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Dectin‑1/CLEC7A Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Mouse Dectin-1/CLEC7A (Catalog # 1756-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Dectin‑1/CLEC7A (Catalog # 1756-DC)

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin-1, respectively. It shares 25-34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

References

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
  4. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
  5. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. 
  6. Willment, J.A. et al. (2003) J. Immunol. 171:4569. 
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Alternate Names

CD369, CLEC7A, CLECSF12, Dectin1

Entrez Gene IDs

64581 (Human); 56644 (Mouse)

Gene Symbol

CLEC7A

UniProt

Q6QLQ4

Additional Dectin-1/CLEC7A Products

Product Documents for Mouse Dectin‑1/CLEC7A Antibody

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Product Specific Notices for Mouse Dectin‑1/CLEC7A Antibody

For research use only

Citations for Mouse Dectin‑1/CLEC7A Antibody

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