Mouse E-Cadherin Biotinylated Antibody
Mouse E-Cadherin Biotinylated Antibody Summary
Accession # P09803
Mouse E-Cadherin Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
E‑Cadherin in A431 Human Cell Line. E-Cadherin was detected in immersion fixed A431 human skin epidermal cell line using Goat Anti-Mouse E-Cadherin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF748) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) 251:1451.
Citations for Mouse E-Cadherin Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Combined mutation of Apc, Kras and Tgfbr2 effectively drives metastasis of intestinal cancer
Authors: E Sakai, M Nakayama, H Oshima, Y Kouyama, A Niida, S Fujii, A Ochiai, KI Nakayama, K Mimori, Y Suzuki, CP Hong, CY Ock, SJ Kim, M Oshima
Cancer Res., 2017-12-27;0(0):.
Sample Types: Whole Tissue
Quantifying cell-generated mechanical forces within living embryonic tissues.
Authors: Campas O, Mammoto T, Hasso S, Sperling R, O'Connell D, Bischof A, Maas R, Weitz D, Mahadevan L, Ingber D
Nat Methods, 2013-12-08;11(2):183-9.
Sample Types: Whole Cells
Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
Authors: Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM
Cell Stem Cell, 2008-07-03;3(1):55-68.
Sample Types: Whole Cells
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