Human/Mouse E-Cadherin Antibody

  (23 citations)
(5 Reviews)
    
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Product Details
Citations (23)
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  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse E-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat
    E-Cadherin is observed and less than 4% cross-reactivity with rhK-Cadherin, rhCadherin-8, rhCadherin-17, and recombinant mouse P-Cadherin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
    Asp157-Val709
    Accession # P09803
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    D3 mouse embryonic stem cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse
E‑Cadherin by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E‑Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
E‑Cadherin in D3 Mouse Embryonic Stem Cell Line. E‑Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse
E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunohistochemistry
E‑Cadherin in Mouse Skin. E‑Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunocytochemistry
E‑Cadherin in Mouse Intestinal Organoids. E‑Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and counterstained with DAPI (blue). Magnification shown at 100X (upper panel) and 40X (lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
E‑Cadherin in Mouse Intestinal Organoids. E‑Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
E‑Cadherin in Mouse Spinal Cord. E‑Cadherin was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human and Mouse E‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

  • References:
    1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
    2. Overduin, M. et al. (1995) Science 267:386.
    3. Takeichi, M. (1991) 251:1451.
  • Entrez Gene IDs:
    999 (Human); 12550 (Mouse); 83502 (Rat)
  • Alternate Names:
    Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Annexin A2 mediates secretion of collagen VI, pulmonary elasticity and apoptosis of bronchial epithelial cells.
    Authors: Dassah M, Almeida D, Hahn R, Bonaldo P, Worgall S, Hajjar K
    J Cell Sci, 2104;127(0):828-44.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Neut
  2. Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination
    Authors: O Nekrasova, RM Harmon, JA Broussard, JL Koetsier, LM Godsel, GN Fitz, ML Gardel, KJ Green
    Nat Commun, 2018;9(1):1053.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes
    Authors: HP Pui, Y Saga
    Mech. Dev., 2018;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Combined mutation of Apc, Kras and Tgfbr2 effectively drives metastasis of intestinal cancer
    Authors: E Sakai, M Nakayama, H Oshima, Y Kouyama, A Niida, S Fujii, A Ochiai, KI Nakayama, K Mimori, Y Suzuki, CP Hong, CY Ock, SJ Kim, M Oshima
    Cancer Res., 2017;0(0):.
  6. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
    Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
    Mucosal Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling
    PLoS Genet, 2016;12(11):e1006442.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  8. A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease
    Nat Microbiol, 2016;2(0):16196.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  9. Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells
    Authors: Y Kato, T Katsuki, H Kokubo, A Masuda, Y Saga
    Nat Commun, 2016;7(0):11272.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed
  10. E-cadherin can limit the transforming properties of activating beta-catenin mutations.
    Authors: Huels D, Ridgway R, Radulescu S, Leushacke M, Campbell A, Biswas S, Leedham S, Serra S, Chetty R, Moreaux G, Parry L, Matthews J, Song F, Hedley A, Kalna G, Ceteci F, Reed K, Meniel V, Maguire A, Doyle B, Soderberg O, Barker N, Watson A, Larue L, Clarke A, Sansom O
    EMBO J, 2015;34(18):2321-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  11. EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury.
    Authors: Kida Y, Ieronimakis N, Schrimpf C, Reyes M, Duffield J
    J Am Soc Nephrol, 2015;24(4):559-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  12. Mitogen-activated protein kinase (MAPK) pathway regulates branching by remodeling epithelial cell adhesion.
    Authors: Ihermann-Hella A, Lume M, Miinalainen I, Pirttiniemi A, Gui Y, Peranen J, Charron J, Saarma M, Costantini F, Kuure S
    PLoS Genet, 2014;10(3):e1004193.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  13. AKT3 regulates ErbB2, ErbB3 and estrogen receptor alpha expression and contributes to endocrine therapy resistance of ErbB2(+) breast tumor cells from Balb-neuT mice.
    Authors: Grabinski N, Mollmann K, Milde-Langosch K, Muller V, Schumacher U, Brandt B, Pantel K, Jucker M
    Cell Signal, 2014;26(5):1021-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  14. Bifidobacterium longum alleviates dextran sulfate sodium-induced colitis by suppressing IL-17A response: involvement of intestinal epithelial costimulatory molecules.
    Authors: Miyauchi, Eiji, Ogita, Tasuku, Miyamoto, Junki, Kawamoto, Seiji, Morita, Hidetosh, Ohno, Hiroshi, Suzuki, Takuya, Tanabe, Soichi
    PLoS ONE, 2013;8(11):e79735.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  15. MMP20 modulates cadherin expression in ameloblasts as enamel develops.
    Authors: Guan X, Bartlett J
    , 2013;92(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  16. A critical role for TLR4 induction of autophagy in the regulation of enterocyte migration and the pathogenesis of necrotizing enterocolitis.
    Authors: Neal M, Sodhi C, Dyer M, Craig B, Good M, Jia H, Yazji I, Afrazi A, Richardson W, Beer-Stolz D, Ma C, Prindle T, Grant Z, Branca M, Ozolek J, Hackam D
    J Immunol, 2013;190(7):3541-51.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC
  17. Epithelial cell-intrinsic notch signaling plays an essential role in the maintenance of gut immune homeostasis.
    Authors: Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H
    J. Immunol., 2012;188(5):2427-36.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  18. N-cadherin+ HSCs in fetal liver exhibit higher long-term bone marrow reconstitution activity than N-cadherin- HSCs.
    Biochem Biophys Res Commun, 2012;428(3):354-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  19. Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli.
    Authors: Betterman K, Paquet-Fifield S, Asselin-Labat M, Visvader J, Butler L, Stacker S, Achen M, Harvey N
    Am J Pathol, 2012;181(6):2225-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  20. Noninvasive assessment of antenatal hydronephrosis in mice reveals a critical role for Robo2 in maintaining anti-reflux mechanism.
    Authors: Wang H, Li Q, Liu J
    PLoS ONE, 2011;6(9):e24763.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  21. Effect of inhibition of vascular endothelial growth factor signaling on distribution of extravasated antibodies in tumors.
    Authors: Nakahara T, Norberg SM, Shalinsky DR, Hu-Lowe DD, McDonald DM
    Cancer Res., 2006;66(3):1434-45.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  22. FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons.
    Authors: Grieshammer U, Cebrian C, Ilagan R, Meyers E, Herzlinger D, Martin GR
    Development, 2005;132(17):3847-57.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  23. Multifunctionality of PAI-1 in fibrogenesis: evidence from obstructive nephropathy in PAI-1-overexpressing mice.
    Authors: Matsuo S, Lopez-Guisa JM, Cai X, Okamura DM, Alpers CE, Bumgarner RE, Peters MA, Zhang G, Eddy AA
    Kidney Int., 2005;67(6):2221-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Expand to show all Citations

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Average Rating: 4.4 (Based on 5 reviews)

Have you used Human/Mouse E-Cadherin Antibody?

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Read what people are saying who have used Human/Mouse E-Cadherin Antibody.

We have 5 reviews tested in 2 species: Human, Mouse.
We have 5 reviews tested in 3 applications: Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Western Blot.

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(3)
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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 IHC Mouse Anonymous 04/13/2018
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Summary

Application Immunohistochemistry
Sample Tested Tumor cell lyastes
Species Mouse
Immunocytochemistry/Immunofluorescence Human/Mouse E-Cadherin Antibody AF748 Array AF748
Enlarge
Very Good
 ICC/IF Human Anonymous 12/19/2017
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Image Details
Immunocytochemistry/Immunofluorescence Human/Mouse E-Cadherin Antibody AF748
Immunocytochemistry/Immunofluorescence: Human/Mouse E-Cadherin Antibody [AF748].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Bladder cancer tissue
Species Human
Immunocytochemistry/Immunofluorescence Human/Mouse E-Cadherin Antibody AF748 Array AF748
Enlarge
Excellent
 ICC/IF Human Anonymous 09/27/2017
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Image Details
Immunocytochemistry/Immunofluorescence Human/Mouse E-Cadherin Antibody AF748
Immunocytochemistry/Immunofluorescence: Human/Mouse E-Cadherin Antibody [AF748].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested iPSC derived epithelial cells
Species Human
  Very Good
 WB Human Anonymous 07/31/2017
Show

Summary

Application Western Blot
Sample Tested 293T human embryonic kidney cell line
Species Human
Western Blot Human/Mouse E‑Cadherin Antibody AF748 Array AF748
Enlarge
Very Good
 WB Mouse Anonymous 04/21/2016
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Image Details
Western Blot Human/Mouse E‑Cadherin Antibody AF748
Western Blot: Human/Mouse E‑Cadherin Antibody [AF748].

Summary

Application Western Blot
Sample Tested mouse primary brain cell cultures
Species Mouse

Other Experimental Details

Other Experimental Details Ab concentration: 0.1 ug/ml

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