Mouse EGFR Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1280

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EGFR in Mouse Embryo.
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Citations (1)
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Mouse EGFR Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EGFR in Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human (rh) EGFR is observed and less than 1% cross‑reactivity with rhErbB2, rhErbB3, and rhErbB4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EGFR
Leu25-Ser647
Accession # Q01279
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse EGFR Fc Chimera (Catalog # 1280-ER)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry EGFR antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

EGFR in Mouse Embryo. EGFR was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EGFR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1280) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as Her1, ErbB1, or ErbB), ErbB2 (Neu, Her2), ErbB3 (Her3), and ErbB4 (Her4). All family members are type I transmembrane glycoproteins. They contain an extracellular ligand binding domain containing two cysteine-rich domains and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The mouse EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 623 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 540 aa cytoplasmic domain (3). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (4-6). Within the ECD, mouse EGFR shares 88% and 93% aa sequence identity with human and rat EGFR, respectively. It shares 44-48% aa sequence identity with the ECD of mouse ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-11). EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (12, 13). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (14).

References
  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Avivi, A. et al. (1991) Oncogene 6:673.
  4. Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
  5. Reiter J.L. et al. (2001) Genomics 71:1.
  6. Xu, Y.H. et al. (1984) Nature 309:806.
  7. Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
  8. Yarden, Y. et al. (1987) Biochemistry 26:1434.
  9. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  10. Lemmon, M.A. et al. (1997) EMBO J. 16:281.
  11. Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
  12. Sibilia, M. and E.F. Wagner (1995) Science 269:234.
  13. Miettinen, P.J. et al. (1995) Nature 376:337.
  14. Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.
Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Citation for Mouse EGFR Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. In situ neutrophil efferocytosis shapes T cell immunity to influenza infection
    Authors: K Lim, TH Kim, A Trzeciak, AM Amitrano, EC Reilly, H Prizant, DJ Fowell, DJ Topham, M Kim
    Nat. Immunol., 2020-08-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse EGF R/ErbB1 Biotinylated Antibody
By Rika Ohkubo on 02/23/2016
Application: Flow Sample Tested: Neural progenitor cells Species: Mouse

I used this antibody with 1:200 dilution (each sample included 10x106 cells).