Mouse EGFR Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse EGFR in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) EGFR and less than 5% cross-reactivity with rhErbB2, rhErbB3, and rhErbB4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse EGFR
    Leu25-Ser647
    Accession # Q9EP98
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    2.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human EGFR by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EGFR at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
EGFR in Mouse Embryo. EGFR was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human EGFR by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EGFR at approximately 180-191 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as Her1, ErbB1, or ErbB), ErbB2 (Neu, Her2), ErbB3 (Her3), and ErbB4 (Her4). All family members are type I transmembrane glycoproteins. They contain an extracellular ligand binding domain containing two cysteine-rich domains and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The mouse EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 623 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 540 aa cytoplasmic domain (3). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (4-6). Within the ECD, mouse EGFR shares 88% and 93% aa sequence identity with human and rat EGFR, respectively. It shares 44-48% aa sequence identity with the ECD of mouse ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-11). EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (12, 13). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (14).

  • References:
    1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
    2. Shilo, B.Z. (2005) Development 132:4017.
    3. Avivi, A. et al. (1991) Oncogene 6:673.
    4. Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
    5. Reiter J.L. et al. (2001) Genomics 71:1.
    6. Xu, Y.H. et al. (1984) Nature 309:806.
    7. Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
    8. Yarden, Y. et al. (1987) Biochemistry 26:1434.
    9. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
    10. Lemmon, M.A. et al. (1997) EMBO J. 16:281.
    11. Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
    12. Sibilia, M. and E.F. Wagner (1995) Science 269:234.
    13. Miettinen, P.J. et al. (1995) Nature 376:337.
    14. Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.
  • Long Name:
    Epidermal Growth Factor Receptor
  • Entrez Gene IDs:
    1956 (Human); 13649 (Mouse)
  • Alternate Names:
    avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. EGF transactivation of Trk receptors regulates the migration of newborn cortical neurons.
    Authors: Puehringer D, Orel N, Luningschror P, Subramanian N, Herrmann T, Chao M, Sendtner M
    Nat Neurosci, 2013;16(4):407-15.
  2. T cells enhance stem-like properties and conditional malignancy in gliomas.
    Authors: Irvin DK, Jouanneau E, Duvall G
    PLoS ONE, 2010;5(6):e10974.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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Normal Goat IgG Control

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