Mouse EGFR Antibody

Catalog # Availability Size / Price Qty
AF1280
AF1280-SP

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Detection of Human EGFR by Western Blot.
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Product Details
Citations (10)
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Mouse EGFR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EGFR in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) EGFR and less than 5% cross-reactivity with rhErbB2, rhErbB3, and rhErbB4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EGFR
Leu25-Ser647
Accession # Q9EP98
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EGFR antibody by Western Blot. View Larger

Detection of Human EGFR by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EGFR at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry EGFR antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

EGFR in Mouse Embryo. EGFR was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Human EGFR antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human EGFR by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EGFR at approximately 180-191 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as Her1, ErbB1, or ErbB), ErbB2 (Neu, Her2), ErbB3 (Her3), and ErbB4 (Her4). All family members are type I transmembrane glycoproteins. They contain an extracellular ligand binding domain containing two cysteine-rich domains and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The mouse EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 623 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 540 aa cytoplasmic domain (3). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (4-6). Within the ECD, mouse EGFR shares 88% and 93% aa sequence identity with human and rat EGFR, respectively. It shares 44-48% aa sequence identity with the ECD of mouse ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-11). EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (12, 13). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (14).

References
  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Avivi, A. et al. (1991) Oncogene 6:673.
  4. Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
  5. Reiter J.L. et al. (2001) Genomics 71:1.
  6. Xu, Y.H. et al. (1984) Nature 309:806.
  7. Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
  8. Yarden, Y. et al. (1987) Biochemistry 26:1434.
  9. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  10. Lemmon, M.A. et al. (1997) EMBO J. 16:281.
  11. Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
  12. Sibilia, M. and E.F. Wagner (1995) Science 269:234.
  13. Miettinen, P.J. et al. (1995) Nature 376:337.
  14. Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.
Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Citations for Mouse EGFR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Single nuclei RNA-seq of mouse placental labyrinth development
    Authors: Bryan Marsh, Robert Blelloch
    eLife
  2. Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
    Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
    Cell Rep, 2020-05-05;31(5):107599.
    Species: Human
    Sample Types:
    Applications: IF
  3. Nanobody-targeting of epidermal growth factor receptor (EGFR) ectodomain variants overcomes resistance to therapeutic EGFR antibodies
    Authors: J Tintelnot, N Baum, C Schultheis, F Braig, M Trentmann, J Finter, W Fumey, P Bannas, B Fehse, K Riecken, K Schütze, C Bokemeyer, T Rösner, T Valerius, M Peipp, F Koch-Nolte, M Binder
    Mol. Cancer Ther., 2019-03-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Epidermal growth factor receptor promotes cerebral and retinal invasion by Toxoplasma gondii
    Authors: YL Corcino, JC Portillo, CS Subauste
    Sci Rep, 2019-01-24;9(1):669.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. The Immunogenicity and Anti-Tumor Efficacy of a Rationally Designed EGFR Vaccine
    Authors: C Cheng, L Deng, R Li
    Cell. Physiol. Biochem., 2018-03-20;46(1):46-56.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  6. iRhom2 promotes lupus nephritis through TNF-? and EGFR signaling
    Authors: X Qing, Y Chinenov, P Redecha, M Madaio, JJ Roelofs, G Farber, PD Issuree, L Donlin, DR McIlwain, TW Mak, CP Blobel, JE Salmon
    J. Clin. Invest., 2018-03-05;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Characterization of 7A7, an anti-mouse EGFR monoclonal antibody proposed to be the mouse equivalent of cetuximab
    Authors: Xuzhi He, Jazmina L. Cruz, Shannon Joseph, Nicola Pett, Hui Yi Chew, Zewen K. Tuong et al.
    Oncotarget
  8. Endocannabinoid Signaling in Embryonic Neuronal Motility and Cell-Cell Contact - Role of mGluR5 and TRPC3 Channels
    Authors: PM Turunen, LM Louhivuori, V Louhivuori, JP Kukkonen, KE Åkerman
    Neuroscience, 2018-02-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. EGF transactivation of Trk receptors regulates the migration of newborn cortical neurons.
    Authors: Puehringer D, Orel N, Luningschror P, Subramanian N, Herrmann T, Chao M, Sendtner M
    Nat Neurosci, 2013-02-17;16(4):407-15.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  10. T cells enhance stem-like properties and conditional malignancy in gliomas.
    Authors: Irvin DK, Jouanneau E, Duvall G
    PLoS ONE, 2010-06-07;5(6):e10974.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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