Mouse Endoglycan/PODXL2 Antibody

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Endoglycan/PODXL2 in Mouse Testis.
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Product Details
Citations (3)
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Mouse Endoglycan/PODXL2 Antibody Summary

Species Reactivity
Detects mouse Endoglycan/PODXL2 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Endoglycan/PODXL2
Accession # Q8CAE9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Endoglycan/PODXL2
Flow Cytometry
2.5 µg/106 cells
bEnd.3 mouse endothelioma cell line
5-15 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Endoglycan/PODXL2 antibody in Mouse Testis by Immunohistochemistry (IHC-Fr). View Larger

Endoglycan/PODXL2 in Mouse Testis. Endoglycan/PODXL2 was detected in perfusion fixed frozen sections of mouse testis using 1.7 µg/mL Goat Anti-Mouse Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3534) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglycan/PODXL2

Endoglycan, also named podocalyxin-like 2 protein, is a type I transmembrane glycoprotein that belongs to the CD34/podocalyxin family of sialomucins that are expressed on endothelial cells and hematopoietic precursors, vascular smooth muscle cells, and some leukocyte subpopulations. The extracellular domain of Endoglycan contains an N-terminal acidic domain with several polyglutamate tracts, a mucin-like domain and a membrane proximal globular domain. Unlike other sialomucins, Endoglycan contains chondroitin sulfate modifications. Within the extracellular domain, mouse endoglycan shares 73% and 96% amino acid sequence identity with human and rat endoglycan, respectively.

Entrez Gene IDs
50512 (Human); 319655 (Mouse)
Alternate Names
Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2

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Citations for Mouse Endoglycan/PODXL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis
    Authors: L Zhang, F Li, P Lei, M Guo, R Liu, L Wang, T Yu, Y Lv, T Zhang, W Zeng, H Lu, Y Zheng
    Journal of animal science and biotechnology, 2021-12-07;12(1):122.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC
  2. Human &alpha-synuclein overexpression in a mouse model of Parkinson's disease leads to vascular pathology, blood brain barrier leakage and pericyte activation
    Authors: O Elabi, A Gaceb, R Carlsson, T Padel, R Soylu-Kuch, I Cortijo, W Li, JY Li, G Paul
    Scientific Reports, 2021-01-13;11(1):1120.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. c-kit delineates a distinct domain of progenitors in the developing kidney.
    Authors: Schmidt-Ott KM, Chen X, Paragas N, Levinson RS, Mendelsohn CL, Barasch J
    Dev. Biol., 2006-07-31;299(1):238-49.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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