Mouse Endoglycan/PODXL2 Antibody

Catalog # Availability Size / Price Qty
AF3534
AF3534-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Endoglycan/PODXL2 in Mouse Testis.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse Endoglycan/PODXL2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Endoglycan/PODXL2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endoglycan/PODXL2
Gly30-Gly498
Accession # Q8CAE9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Endoglycan/PODXL2
Flow Cytometry
2.5 µg/106 cells
bEnd.3 mouse endothelioma cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Endoglycan/PODXL2 antibody in Mouse Testis by Immunohistochemistry (IHC-Fr). View Larger

Endoglycan/PODXL2 in Mouse Testis. Endoglycan/PODXL2 was detected in perfusion fixed frozen sections of mouse testis using 1.7 µg/mL Goat Anti-Mouse Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3534) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglycan/PODXL2

Endoglycan, also named podocalyxin-like 2 protein, is a type I transmembrane glycoprotein that belongs to the CD34/podocalyxin family of sialomucins that are expressed on endothelial cells and hematopoietic precursors, vascular smooth muscle cells, and some leukocyte subpopulations. The extracellular domain of Endoglycan contains an N-terminal acidic domain with several polyglutamate tracts, a mucin-like domain and a membrane proximal globular domain. Unlike other sialomucins, Endoglycan contains chondroitin sulfate modifications. Within the extracellular domain, mouse endoglycan shares 73% and 96% amino acid sequence identity with human and rat endoglycan, respectively.

Entrez Gene IDs
50512 (Human); 319655 (Mouse)
Alternate Names
Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2

Product Datasheets

You must select a language.

x

Citations for Mouse Endoglycan/PODXL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis
    Authors: L Zhang, F Li, P Lei, M Guo, R Liu, L Wang, T Yu, Y Lv, T Zhang, W Zeng, H Lu, Y Zheng
    Journal of animal science and biotechnology, 2021-12-07;12(1):122.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC
  2. Human &alpha-synuclein overexpression in a mouse model of Parkinson's disease leads to vascular pathology, blood brain barrier leakage and pericyte activation
    Authors: O Elabi, A Gaceb, R Carlsson, T Padel, R Soylu-Kuch, I Cortijo, W Li, JY Li, G Paul
    Scientific Reports, 2021-01-13;11(1):1120.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. c-kit delineates a distinct domain of progenitors in the developing kidney.
    Authors: Schmidt-Ott KM, Chen X, Paragas N, Levinson RS, Mendelsohn CL, Barasch J
    Dev. Biol., 2006-07-31;299(1):238-49.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse Endoglycan/PODXL2 Antibody

There are currently no reviews for this product. Be the first to review Mouse Endoglycan/PODXL2 Antibody and earn rewards!

Have you used Mouse Endoglycan/PODXL2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review