Mouse Erythropoietin Antibody

(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Erythropoietin in direct ELISAs. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant rat Erythropoietin, 5-15% cross-reactivity with recombinant human Erythropoietin, and no cross-reactivity with recombinant mouse Thrombopoietin is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 148436
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Erythropoietin    
    Ala27-Arg192
    Accession # P07321
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Mouse Erythropoietin Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse Erythropoietin Antibody (Catalog # MAB9591)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Mouse Erythropoietin Biotinylated Antibody (Catalog # BAM9592)
  • ELISA Standard
     
    Recombinant Mouse Erythropoietin Protein (Catalog # 959-ME)
  • Neutralization
    Measured by its ability to neutralize Erythropoietin-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 10 ng/mL Recombinant Mouse Erythropoietin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by Erythropoietin and Neutralization by Mouse Erythropoietin Antibody. Recombinant Mouse Erythropoietin (Catalog # 959‑ME) stimulates proliferation in the TF‑1 human erythro­leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom­binant Mouse Erythropoietin (10 ng/mL) is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse Erythropoietin Monoclonal Antibody (Catalog # MAB9591). The ND50 is typically 0.15‑0.75 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin

Erythropoietin (Epo) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N‑glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of Epo is required for biological activities in vivo (4). Mature mouse Epo shares 95% amino acid sequence identity with rat Epo and 73%‑82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6‑8). Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R) (8, 9). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of Epo R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). Epo additionally plays a tissue‑protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

  • References:
    1. Koury, M. J. (2005) Exp. Hematol. 33:1263.
    2. Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
    3. Wen, D. et al. (1993) Blood 82:1507.
    4. Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
    5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
    6. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
    7. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
    8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
    9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
    10. Acs, G. et al. (2001) Cancer Res. 61:3561.
    11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
    12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
    13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.
  • Entrez Gene IDs:
    2056 (Human); 13856 (Mouse); 24335 (Rat)
  • Alternate Names:
    EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2
Related Research Areas
Isotype Controls
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Rat IgG2A Isotype Control

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Secondary Antibodies
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Goat Anti-Rat IgG Antibody

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Rat IgG VisUCyte HRP Polymer Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Affinity Purification.

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Summary

ApplicationAffinity Purification
Sample Tested3T3-L1 mouse embryonic fibroblast adipose-like cell line
SpeciesMouse

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