Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 148436
View all Erythropoietin/EPO Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Erythropoietin/EPO
Ala27-Arg192
Accession # P07321

Specificity

Detects mouse Erythropoietin/EPO in direct ELISAs. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant rat Erythropoietin/EPO, 5-15% cross-reactivity with recombinant human Erythropoietin/EPO, and no cross-reactivity with recombinant mouse Thrombopoietin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Erythropoietin/EPO Antibody

Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Mouse Erythropoietin Antibody.

Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Mouse Erythropoietin Antibody.

Recombinant Mouse Erythropoietin/EPO (Catalog # 959-ME) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB9591). The ND50 is typically 0.15-0.75 µg/mL.

Applications for Mouse Erythropoietin/EPO Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 10 ng/mL Recombinant Mouse Erythropoietin/EPO.

Mouse Erythropoietin/EPO Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Erythropoietin/EPO Biotinylated Antibody (Catalog # BAM9592)
  • Standard: Recombinant Mouse Erythropoietin/EPO Protein (Catalog # 959-ME)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB9591 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Erythropoietin/EPO

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N‑glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of EPO is required for biological activities in vivo (4). Mature mouse EPO shares 95% amino acid sequence identity with rat EPO and 73%‑82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6‑8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue‑protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

References

  1. Koury, M. J. (2005) Exp. Hematol. 33:1263.
  2. Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  7. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.

Alternate Names

ECYT5, EPO, MVCD2

Entrez Gene IDs

2056 (Human); 13856 (Mouse); 24335 (Rat)

Gene Symbol

EPO

UniProt

P07321

Additional Erythropoietin/EPO Products

Product Documents for Mouse Erythropoietin/EPO Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Erythropoietin/EPO Antibody

For research use only

Citations for Mouse Erythropoietin/EPO Antibody

Customer Reviews for Mouse Erythropoietin/EPO Antibody (2)

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Showing  1 - 2 of 2 reviews Showing All
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  • Mouse Erythropoietin Antibody
    Name: Leslie Priddy
    Application: Immunohistochemistry
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 04/13/2018
  • Mouse Erythropoietin Antibody
    Name: Anonymous
    Application: Affinity Purification
    Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line
    Species: Mouse
    Verified Customer | Posted 03/15/2016

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