Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N‑glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of EPO is required for biological activities in vivo (4). Mature mouse EPO shares 95% amino acid sequence identity with rat EPO and 73%‑82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6‑8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue‑protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).
Mouse Erythropoietin/EPO Antibody (148436)
R&D Systems | Catalog # MAB9591
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 148436
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Erythropoietin/EPO
Ala27-Arg192
Accession # P07321
Ala27-Arg192
Accession # P07321
Specificity
Detects mouse Erythropoietin/EPO in direct ELISAs. In direct ELISAs, approximately 25-50%
cross-reactivity with recombinant rat Erythropoietin/EPO, 5-15% cross-reactivity
with recombinant human Erythropoietin/EPO, and no cross-reactivity with recombinant
mouse Thrombopoietin is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse Erythropoietin/EPO Antibody (148436)
Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Mouse Erythropoietin Antibody.
Recombinant Mouse Erythropoietin/EPO (Catalog # 959-ME) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB9591). The ND50 is typically 0.15-0.75 µg/mL.
Mouse Erythropoietin ELISA Standard Curve
Recombinant Mouse Erythropoietin (Catalog # 959-ME) was serially diluted and captured by Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB9591) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Erythropoietin/EPO Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse Erythropoietin/EPO Antibody (148436)
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 10 ng/mL Recombinant Mouse Erythropoietin/EPO.
Mouse Erythropoietin/EPO Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB9591 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Erythropoietin/EPO
References
- Koury, M. J. (2005) Exp. Hematol. 33:1263.
- Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
- Wen, D. et al. (1993) Blood 82:1507.
- Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
- Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
- Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
- Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
- Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
- Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
- Acs, G. et al. (2001) Cancer Res. 61:3561.
- Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
- Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
- Kertesz, N. et al. (2004) Dev. Biol. 276:101.
Alternate Names
ECYT5, EPO, MVCD2
Gene Symbol
EPO
UniProt
Additional Erythropoietin/EPO Products
Product Documents for Mouse Erythropoietin/EPO Antibody (148436)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Erythropoietin/EPO Antibody (148436)
For research use only
Related Research Areas
Citations for Mouse Erythropoietin/EPO Antibody (148436)
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Customer Reviews for Mouse Erythropoietin/EPO Antibody (148436) (2)
5 out of 5
2 Customer Ratings
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Application: ImmunohistochemistrySample Tested: SerumSpecies: MouseVerified Customer | Posted 04/13/2018
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