Mouse ESGP Antibody

Catalog # Availability Size / Price Qty
AF4580
AF4580-SP
Detection of Mouse ESGP by Western Blot.
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Product Details
Citations (12)
FAQs
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Mouse ESGP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ESGP in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse ESGP
Leu24-Lys84
Accession # Q2Q5T5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse ESGP antibody by Western Blot. View Larger

Detection of Mouse ESGP by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ESGP at approximately 9 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry ESGP antibody in Mouse Testis by Immunohistochemistry (IHC-Fr). View Larger

ESGP in Mouse Testis. ESGP was detected in perfusion fixed frozen sections of mouse testis using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to late spermatids. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunocytochemistry ESGP antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

ESGP in D3 Mouse Cell Line. ESGP was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ESGP

ESGP (ES cell and germ cell-specific protein) is a presumably secreted polypeptide that belongs to no known protein family. It is expressed specifically in pluripotential cell types, and does not appear to be involved in differentiation. Mouse ESGP precursor is 84 amino acids (aa) in length. It contains a 25 aa signal sequence with a 59 aa mature segment. There are no N-linked glycosylation sites. One potential 108 aa variant shows an alternate start site 24 aa upstream of the standard start site.

Long Name
Embryonic Stem Cell and Germ Cell Specific Protein
Entrez Gene IDs
653016 (Mouse)
Alternate Names
EG653016; EG653016;Gm7325;m;minion;Mymx;myomer;myomerger;Protein myomixer; ESGP

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Citations for Mouse ESGP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Myomaker and Myomerger Work Independently to Control Distinct Steps of Membrane Remodeling during Myoblast Fusion
    Authors: Evgenia Leikina, Dilani G. Gamage, Vikram Prasad, Joanna Goykhberg, Michael Crowe, Jiajie Diao et al.
    Developmental Cell
  2. Filopodia powered by class x myosin promote fusion of mammalian myoblasts
    Authors: Hammers DW, Hart CC, Matheny MK et al.
    eLife
  3. Survival motor neuron deficiency slows myoblast fusion through reduced myomaker and myomixer expression
    Authors: Nikki M. McCormack, Eric Villalón, Coralie Viollet, Anthony R. Soltis, Clifton L. Dalgard, Christian L. Lorson et al.
    Journal of Cachexia, Sarcopenia and Muscle
  4. Formation of Aberrant Myotubes by Myoblasts Lacking Myosin VI Is Associated with Alterations in the Cytoskeleton Organization, Myoblast Adhesion and Fusion
    Authors: Lilya Lehka, Małgorzata Topolewska, Dominika Wojton, Olena Karatsai, Paloma Alvarez-Suarez, Paweł Pomorski et al.
    Cells
  5. Interleukin‐4 administration improves muscle function, adult myogenesis, and lifespan of colon carcinoma‐bearing mice
    Authors: Domiziana Costamagna, Robin Duelen, Fabio Penna, Detlef Neumann, Paola Costelli, Maurilio Sampaolesi
    Journal of Cachexia, Sarcopenia and Muscle
  6. IL-4 Signaling Promotes Myoblast Differentiation and Fusion by Enhancing the Expression of MyoD, Myogenin, and Myomerger
    Authors: Kurosaka, M;Hung, YL;Machida, S;Kohda, K;
    Cells
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Phosphatidylserine orchestrates Myomerger membrane insertions to drive myoblast fusion
    Authors: DG Gamage, K Melikov, P Munoz-Tell, TJ Wherley, LC Focke, E Leikina, E Huffman, J Diao, DJ Kojetin, V Prasad, LV Chernomord, DP Millay
    Proceedings of the National Academy of Sciences of the United States of America, 2022-09-12;119(38):e2202490119.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  8. LncRNA OIP5-AS1-directed miR-7 degradation promotes MYMX production during human myogenesis
    Authors: JH Yang, MW Chang, D Tsitsipati, X Yang, JL Martindale, R Munk, A Cheng, E Izydore, PR Pandey, Y Piao, K Mazan-Mamc, S De, K Abdelmohse, M Gorospe
    Nucleic Acids Research, 2022-07-08;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Magnesium Homeostasis in Myogenic Differentiation-A Focus on the Regulation of TRPM7, MagT1 and SLC41A1 Transporters
    Authors: M Zocchi, L Locatelli, GV Zuccotti, A Mazur, D Béchet, JA Maier, S Castiglion
    International Journal of Molecular Sciences, 2022-01-31;23(3):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Fusogenic micropeptide Myomixer is essential for satellite cell fusion and muscle regeneration
    Authors: P Bi, JR McAnally, JM Shelton, E Sánchez-Or, R Bassel-Dub, EN Olson
    Proc. Natl. Acad. Sci. U.S.A., 2018-03-26;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. The microprotein Minion controls cell fusion and muscle formation
    Authors: Q Zhang, AA Vashisht, J O'Rourke, SY Corbel, R Moran, A Romero, L Miraglia, J Zhang, E Durrant, C Schmedt, SC Sampath, SC Sampath
    Nat Commun, 2017-06-01;8(0):15664.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  12. Myomerger induces fusion of non-fusogenic cells and is required for skeletal muscle development
    Authors: ME Quinn, Q Goh, M Kurosaka, DG Gamage, MJ Petrany, V Prasad, DP Millay
    Nat Commun, 2017-06-01;8(0):15665.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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