Mouse Fetuin A/AHSG Antibody

Catalog # Availability Size / Price Qty
AF1563
AF1563-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Mouse Fetuin A/AHSG Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fetuin A/AHSG in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human Fetuin A/AHSG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fetuin A/AHSG (R&D Systems, Catalog # 1563-PI)
Ala19-Ile345
Accession # NP_038493
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Fetuin A/AHSG (Catalog # 1563-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin A/AHSG

Mouse Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It has been also called "countertrypsin" because of its ability to inhibit trypsin (1). It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (2, 3). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (4). The major form of plasma Fetuin A corresponds to two disulfide bond-linked chains derived from the single chain (5). Fetuin-A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (7, 8).

References
  1. Yamamoto, K. and H. Sinohara (1993) J. Biol. Chem. 268:17750.
  2. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
  3. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
  4. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
  5. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta 671:78.
  6. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
  7. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
  8. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
Long Name
alpha-2-HS Glycoprotein
Entrez Gene IDs
197 (Human); 11625 (Mouse); 25373 (Rat)
Alternate Names
A2HS; AHS; AHSG; alpha-2-HS-glycoprotein; alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; FETUAba-alpha-2-glycoprotein; Fetuin A; fetuin-A; HSGA

Product Datasheets

Citation for Mouse Fetuin A/AHSG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Fetuin-A deficiency protects mice from Experimental Autoimmune Encephalomyelitis (EAE) and correlates with altered innate immune response
    Authors: VK Harris, L Bell, RA Langan, J Tuddenham, M Landy, SA Sadiq
    PLoS ONE, 2017;12(4):e0175575.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Mouse Fetuin A/AHSG Antibody
By Anonymous on 11/22/2020
Application: Functional Assay Sample Tested: Plasma Species: Mouse