Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 113308
View all Flt-3/Flk-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Flt‑3/Flk‑2
Asn28-Ser544 (predicted)
Accession # Q00342

Specificity

Detects mouse Flt‑3/Flk‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Flt-3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Flt‑3/Flk‑2 Antibody

Detection of Flt‑3/Flk‑2 in M1 cells by Flow Cytometry

M1 cells were stained with Rat Anti-Mouse Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # mab7681, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Flt‑3/Flk‑2 antibody in M1 Mouse Cell Line by Immunocytochemistry (ICC).

Flt‑3/Flk‑2 in M1 Mouse Cell Line.

Flt-3/Flk-2 was detected in immersion fixed M1 mouse myeloid leukemia cell line using Rat Anti-Mouse Flt-3/Flk-2 Monoclonal Antibody (Catalog # MAB7681) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Flt‑3/Flk‑2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: M1 mouse myeloid leukemia cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed M1 mouse myeloid leukemia cell line

Reviewed Applications

Read 2 reviews rated 3.5 using MAB7681 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1 (stem cell tyrosine kinase) and designated CD135, is a member of the class III subfamily of receptor tyrosine kinases. This familay includes KIT, the receptor for SCF, and C-FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Mouse Flt-3 cDNA encodes a 992 amino acid (aa) residue type I membrane protein with a 27 aa residue signal peptide, a 517 aa extracellular domain with 10 potential N-linked glycosylation sites, a 20 aa residue transmembrane domain and a 428 aa residue cytoplasmic domain. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3 Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

References

  1. Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
  2. Drexler, H.G. (1996) Leukemia 10:588.

Long Name

fms-like Tyrosine Kinase 3

Alternate Names

CD135, Flk-2, Flt3, STK-1

Entrez Gene IDs

2322 (Human); 14255 (Mouse)

Gene Symbol

FLT3

UniProt

Q00342

Additional Flt-3/Flk-2 Products

Product Documents for Mouse Flt‑3/Flk‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Flt‑3/Flk‑2 Antibody

For research use only

Citations for Mouse Flt‑3/Flk‑2 Antibody

Customer Reviews for Mouse Flt‑3/Flk‑2 Antibody (2)

3.5 out of 5
2 Customer Ratings
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Customer Images

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  • Mouse Flt-3/Flk-2 Antibody
    Name: Anonymous
    Application: Immunofluorescence - Fixed-frozen
    Sample Tested: bone marrow
    Species: Mouse
    Verified Customer | Posted 10/21/2018
    few CD135+ cells with membranous staining. Rather high background, especially in granulocytes.
    Whole Femur Bone Marrow Sections, Fixed-Frozen / Decalcified
    Mouse Flt‑3/Flk‑2 Antibody MAB7681
  • Mouse Flt-3/Flk-2 Antibody
    Name: Adam Guess
    Application: Flow Cytometry
    Sample Tested: mouse AML cells
    Species: Mouse
    Verified Customer | Posted 11/08/2016
    Mouse Flt‑3/Flk‑2 Antibody MAB7681

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Protocols

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