Mouse Frizzled-3 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM1001
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Mouse Frizzled-3 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-3 in direct ELISAs and Western blots. In flow cytometry and immunohistochemistry, also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and on human HEK 293 cells. In direct ELISAs, no cross‑reactivity with Frizzled-1, -4, -7 or -8 is observed.
Source
Monoclonal Rat IgG2A Clone # 169310
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
Met1-Val157
Accession # Q61086
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Frizzled‑3
Flow Cytometry
2.5 µg/106 cells
HEK293 human embryonic kidney cell line
Immunohistochemistry
8-25 µg/mL
Immersion fixed frozen sections of mouse embryo (E13-15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-3

Frizzled-3 is a member of the Frizzled family of G-protein coupled receptors that have seven transmembrane domains and an extracellular CRD. Frizzled proteins function as receptors for Wnt proteins.

Entrez Gene IDs
7976 (Human); 14365 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3

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