Mouse Galectin-1 DuoSet ELISA

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Catalog # Availability Size / Price Qty
DY1245
Ancillary Products Available

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Mouse Galectin-1 ELISA Standard Curve
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Product Details
Procedure
Citations (10)
FAQs
Supplemental Products
Reviews

Mouse Galectin-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
125.0 - 8,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Galectin-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

Mouse Galectin-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-1

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Entrez Gene IDs:
3956 (Human); 16852 (Mouse)
Alternate Names:
14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse Galectin-1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. A Novel P53 Nanomedicine Reduces Immunosuppression and Augments Anti-PD-1 Therapy for Non-Small Cell Lung Cancer in Syngeneic Mouse Models
    Authors: SS Kim, JB Harford, M Moghe, C Doherty, EH Chang
    Cells, 2022-10-31;11(21):.
    Species: Mouse
    Sample Types: Plasma
  2. A TLR4-independent critical role for CD14 in intracellular LPS sensing
    Authors: SO Vasudevan, AJ Russo, P Kumari, SK Vanaja, VA Rathinam
    Cell Reports, 2022-05-03;39(5):110755.
    Species: Mouse
    Sample Types: Plasma
  3. Hierarchical cell-type-specific functions of caspase-11 in LPS shock and antibacterial host defense
    Authors: P Kumari, AJ Russo, SS Wright, S Muthupalan, VA Rathinam
    Cell Reports, 2021-04-20;35(3):109012.
    Species: Mouse
    Sample Types: Plasma
  4. A Pro-resolving Role for Galectin-1 in Acute Inflammation
    Authors: HL Law, RD Wright, AJ Iqbal, LV Norling, D Cooper
    Front Pharmacol, 2020-03-20;11(0):274.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  5. Galectin-1 drives lymphoma CD20 immunotherapy resistance: validation of a preclinical system to identify resistance mechanisms
    Authors: JM Lykken, M Horikawa, V Minard-Col, M Kamata, T Miyagaki, JC Poe, TF Tedder
    Blood, 2016-02-17;127(15):1886-95.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Galectin-1 Protein Therapy Prevents Pathology and Improves Muscle Function in the mdx Mouse Model of Duchenne Muscular Dystrophy.
    Authors: Van Ry P, Wuebbles R, Key M, Burkin D
    Mol Ther, 2015-06-08;23(8):1285-97.
    Species: Mouse
    Sample Types: Serum
  7. Galectin-1 in early acute myocardial infarction.
    Authors: Al-Salam S, Hashmi S
    PLoS ONE, 2014-01-31;9(1):e86994.
    Species: Mouse
    Sample Types: Plasma
  8. Lupus-prone strains vary in susceptibility to antibody-mediated end organ disease.
    Authors: Xie, C, Du, Y, Kumar, K, Li, L, Han, J, Liu, K, Zhou, X J, Mohan, C
    Genes Immun, 2013-02-07;14(3):170-8.
    Species: Mouse
    Sample Types: Serum
  9. Tumor microenvironment-derived proteins dominate the plasma proteome response during breast cancer induction and progression.
    Authors: Pitteri SJ, Kelly-Spratt KS, Gurley KE
    Cancer Res., 2011-06-08;71(15):5090-100.
    Species: Mouse
    Sample Types: Plasma
  10. Galectin-1 promotes immunoglobulin production during plasma cell differentiation.
    Authors: Tsai CM, Chiu YK, Hsu TL, Lin IY, Hsieh SL, Lin KI
    J. Immunol., 2008-10-01;181(7):4570-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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