Mouse GITR Ligand/TNFSF18 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse GITR Ligand/TNFSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human GITR Ligand, recombinant mouse (rm) CD40, or rmTNF-alpha is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 337122
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse GITR Ligand/TNFSF18
    Thr47-Ser173
    Accession # Q80YG2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Mouse GITR Ligand/TNFSF18 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse GITR Ligand/TNFSF18 Antibody (Catalog # MAB2177)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse GITR Ligand/TNFSF18 Biotinylated Antibody (Catalog # BAF2177)
  • ELISA Standard
     
    Recombinant Mouse GITR Ligand/TNFSF18 (aa 47-173) Protein (Catalog # 2177-GL)
  • Neutralization
    Measured by its ability to neutralize GITR Ligand/TNFSF18-induced proliferation in mouse CD4+ T cells. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 2 µg/mL Recombinant Mouse GITR Ligand/TNFSF18, 0.5 µg/mL Mouse CD3 epsilon Monoclonal Antibody, and 10 µg/mL of a cross-linking antibody, polyHistidine Monoclonal Antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by GITR Ligand/TNFSF18 and Neutralization by Mouse GITR Ligand/TNFSF18 Antibody. In the presence of Mouse CD3 epsilon Monoclonal Anti­body (0.5 µg/mL, Catalog # MAB484) and a cross-linking antibody, polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (Catalog # 2177-GL) stimulates proliferation in mouse CD4+ T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/
TNFSF18 Monoclonal Antibody (Catalog # MAB2177). The ND50 is typically 2-8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GITR Ligand/TNFSF18

Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) residues type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently up-regulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25+ regulatory CD4+ T cells (Treg). GITR is also expressed on naïve CD4+ CD25- T cells, where its expression is up-regulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides co-stimulatory signals for activated CD4+ CD25- T cells to enhance cell proliferation and augment cytokine production. On CD4+ CD25+ Treg cells, GITRL also provides co-stimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important co-stimulatory signals that play important roles in modulating diverse T cell functions (1‑4).

  • References:
    1. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
    2. Ji, H. et al. (2004) J. Immunology 172:5823.
    3. Kanamaru, F. et al. (2004) J. Immunology 172:7306.
    4. Ronchetti, S. et al. (2004) Eur. J. Immunology 34:613.
  • Long Name:
    Glucocorticoid Induced TNF Receptor Family Related Gene Ligand
  • Entrez Gene IDs:
    8995 (Human); 240873 (Mouse); 364031 (Rat)
  • Alternate Names:
    Activation-inducible TNF-related ligand; AITRLMGC138237; GITR Ligand; GITRL; GITRLglucocorticoid-induced TNFR-related protein ligand; Glucocorticoid-induced TNF-related ligand; hGITRLGITR ligand; TL6AITR ligand; TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.
    Authors: Mahmud, Shawn A, Manlove, Luke S, Schmitz, Heather, Xing, Yan, Wang, Yanyan, Owen, David L, Schenkel, Jason M, Boomer, Jonathan, Green, Jonathan, Yagita, Hideo, Chi, Hongbo, Hogquist, Kristin, Farrar, Michael
    Nat Immunol, 2014;15(5):473-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Neut
Isotype Controls
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Rat IgG2A Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Flow F0113 2  
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Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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