Mouse gp130 Antibody (125605)

Name: Mouse gp130 Antibody (125605)
Brand: R&D Systems
SKU: MAB4682
Price: 469 USD
Availability: InStock

R&D Systems | Catalog # MAB4682

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG₁ Clone # 125605

View all gp130 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse gp130
Gln23-Glu617
Accession # Q00560

Specificity

Detects mouse gp130 ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human gp130, recombinant mouse (rm) rmIL-6, or rmIL-6R is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse gp130 Antibody (125605)

Cell Proliferation Induced by Human IL‑6 and Neutralization by Mouse gp130 Antibody.

Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the 7TD1 mouse hybridoma line. (orange line). Proliferation elicited by Recombinant Human IL-6 (25 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4682). The ND₅₀ is typically 0.15-1 µg/mL.

Mouse gp130 ELISA Standard Curve

Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG) was serially diluted and captured by Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4682) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF468) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse gp130 Antibody (125605)

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize IL-6-mediated proliferation of the 7TD1 mouse hybridoma line. The Neutralization Dose (ND₅₀) is typically 0.15-1 µg/mL in the presence of 25 pg/mL Recombinant Human IL-6 (Catalog # 206-IL).

Mouse gp130 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse gp130 Biotinylated Antibody (Catalog # BAF468)
Standard: Recombinant Mouse gp130 Fc Chimera Protein, CF (Catalog # 468-MG)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: gp130

gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R. gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.