Recombinant Mouse gp130 Fc Chimera Protein, CF (Catalog # 468-MG)
Measured by its ability to neutralize IL‑6 R alpha -induced growth inhibition in the M1 mouse myeloid leukemia cell line. Saito, T. et al. (1991) J. Immunol. 147:168. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Growth Inhibition Induced by IL‑6 R alpha and Neutralization by Mouse gp130 Antibody. Recombinant Human IL‑6 R alpha (Catalog # 227-SR) induces growth inhibition in the M1 mouse myeloid leukemia cell line in the presence of 30 ng/mL of Recombinant Human IL-6 (Catalog # 206-IL) in a dose-dependent manner (orange line), as measured 3H-Thymidine incorporation. Growth Inhibition elicited by Recombinant Human IL‑6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse gp130 Monoclonal Antibody (Catalog # MAB4682). The ND50 is typically 0.02-0.1 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R. gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.
Narazaki, M. et al. (1993) Blood 82:1120.
Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
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