Mouse gp130 Antibody

Catalog # Availability Size / Price Qty
AF468
AF468-SP
Mouse gp130 Antibody in Neutralization
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Product Details
Citations (3)
FAQs
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Mouse gp130 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse gp130 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human gp130 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse gp130
Gln23-Glu617
Accession # Q6PDI9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG)
Flow Cytometry
2.5 µg/106 cells
M1 mouse myeloid leukemia cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑6-induced inhibition of proliferation in the M1 mouse myeloid leukemia cell line. Saito, T. et al. (1991) J. Immunol. 147:168. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 20 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IL-6 Inhibition of Cell Proliferation and Neutralization by Mouse gp130 Antibody. View Larger

IL-6 Inhibition of Cell Proliferation and Neutralization by Mouse gp130 Antibody. Recombinant Human IL‑6 (Catalog # 206-IL) inhibits proliferation in the the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human IL‑6 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF468). The ND50 is typically 0.3-1.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References
  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

Product Datasheets

Citations for Mouse gp130 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cachexia-associated adipose loss induced by tumor-secreted leukemia inhibitory factor is counterbalanced by decreased leptin
    Authors: GK Arora, A Gupta, S Narayanan, T Guo, P Iyengar, RE Infante
    JCI Insight, 2018;3(14):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Innate Stat3-mediated induction of the antimicrobial protein Reg3gamma is required for host defense against MRSA pneumonia.
    Authors: Choi S, McAleer J, Zheng M, Pociask D, Kaplan M, Qin S, Reinhart T, Kolls J
    J Exp Med, 2013;210(3):551-61.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation.
    Authors: Fredj S, Bescond J, Louault C, Delwail A, Potreau D
    J. Cell. Physiol., 2005;204(2):428-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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Secondary Antibodies

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