Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG)
2.5 µg/106 cells
M1 mouse myeloid leukemia cell line
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize IL‑6-induced inhibition of proliferation in the M1 mouse myeloid leukemia cell line. Saito, T. et al. (1991) J. Immunol. 147:168. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 20 ng/mL Recombinant Human IL‑6.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL-6 Inhibition of Cell Proliferation and Neutralization by Mouse gp130 Antibody. Recombinant Human IL‑6 (Catalog # 206-IL) inhibits proliferation in the the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human IL‑6 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF468). The ND50 is typically 0.3-1.0 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.
Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.
Narazaki, M. et al. (1993) Blood 82:1120.
Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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