Mouse GRIN1/NMDAR1 Antibody (1031915)

R&D Systems | Catalog # MAB10655

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Intracellular Staining by Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 1031915
View all GRIN1/NMDAR1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GRIN1/NMDAR1
Met1-Gln559

Specificity

Detects mouse GRIN1/NMDAR1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse GRIN1/NMDAR1 Antibody (1031915)

GRIN1/NMDAR1 in NS0 Mouse Cell Line Transfected with Mouse GRIN1/NMDAR1.

GRIN1/NMDAR1 was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse GRIN1/NMDAR1 (positive staining) and NS0 mouse myeloma cell line (wild type, negative control) using Rat Anti-Mouse GRIN1/NMDAR1 Monoclonal Antibody (Catalog # MAB10655) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse GRIN1/NMDAR1 in NS0 cells transfected with Mouse GRIN1/NMDAR1 by Flow Cytometry.

NS0 cells transfected with mouse GRIN1/NMDAR1 (filled histogram) or irrelevant protein (open histogram) were stained with Rat Anti-Mouse GRIN1/NMDAR1 Monoclonal Antibody (Catalog # MAB10655) or Rat IgG2A Isotype Control Antibody (MAB006, data not shown) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012).

Applications for Mouse GRIN1/NMDAR1 Antibody (1031915)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NS0 mouse myeloma cell line transfected with mouse GRIN1/NMDAR1

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: NS0 cells transfected with Mouse GRIN1/NMDAR1

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GRIN1/NMDAR1

Grin-1 is a critical component of NMDA receptors.  Expressed in the brain, these components play a key role in plasticity of synapses, which is believed to underlie memory and learning.  Missense variants of the receptor components cause similar syndromes with varying severity of intellectual impairment, autism, epilepsy, and motor dysfunction.  In Mice with reduced NMDA receptor activity, schizophrenia-like behaviors are revealed.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 1

Alternate Names

GluN1, MRD8, NMD-R1, NMDA R, NR1 Subunit, NMDA1, NMDAR1

Entrez Gene IDs

2902 (Human); 14810 (Mouse); 24408 (Rat)

Gene Symbol

GRIN1

Additional GRIN1/NMDAR1 Products

Product Documents for Mouse GRIN1/NMDAR1 Antibody (1031915)

Certificate of Analysis

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Product Specific Notices for Mouse GRIN1/NMDAR1 Antibody (1031915)

For research use only

Citations for Mouse GRIN1/NMDAR1 Antibody (1031915)

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