Mouse GRIN1/NMDAR1 Antibody

Catalog # Availability Size / Price Qty
MAB10655-100
MAB10655-SP
GRIN1/NMDAR1 in NS0 Mouse Cell Line Transfected with Mouse GRIN1/NMDAR1.
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Mouse GRIN1/NMDAR1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GRIN1/NMDAR1 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 1031915
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GRIN1/NMDAR1
Met1-Gln559
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
Immersion fixed NS0 mouse myeloma cell line transfected with mouse GRIN1/NMDAR1
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
NS0 cells transfected with Mouse GRIN1/NMDAR1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

GRIN1/NMDAR1 in NS0 Mouse Cell Line Transfected with Mouse GRIN1/NMDAR1. GRIN1/NMDAR1 was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse GRIN1/NMDAR1 (positive staining) and NS0 mouse myeloma cell line (wild type, negative control) using Rat Anti-Mouse GRIN1/NMDAR1 Monoclonal Antibody (Catalog # MAB10655) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry View Larger

Detection of Mouse GRIN1/NMDAR1 in NS0 cells transfected with Mouse GRIN1/NMDAR1 by Flow Cytometry. NS0 cells transfected with mouse GRIN1/NMDAR1 (filled histogram) or irrelevant protein (open histogram) were stained with Rat Anti-Mouse GRIN1/NMDAR1 Monoclonal Antibody (Catalog # MAB10655) or Rat IgG2A Isotype Control Antibody (MAB006, data not shown) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRIN1/NMDAR1

Grin-1 is a critical component of NMDA receptors.  Expressed in the brain, these components play a key role in plasticity of synapses, which is believed to underlie memory and learning.  Missense variants of the receptor components cause similar syndromes with varying severity of intellectual impairment, autism, epilepsy, and motor dysfunction.  In Mice with reduced NMDA receptor activity, schizophrenia-like behaviors are revealed.

Long Name
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 1
Entrez Gene IDs
2902 (Human); 14810 (Mouse); 24408 (Rat)
Alternate Names
GluN1; GRIN1; MRD8; NDHMSD; NDHMSR;NMD-R1; NMDA R, NR1 Subunit; NMDA1; NMDAR1; NMD-R1; NR1

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