Key Product Details

Species Reactivity

Mouse

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 944213
View all IFN-beta Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived Mouse IFN‑ beta
Ile22-Asn182
Accession # P01575

Specificity

Detects Mouse IFN‑ beta in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse IFN‑ beta Antibody

Mouse IFN‑beta ELISA Standard Curve.

Recombinant Mouse IFN‑beta protein was serially diluted 2-fold and captured by Rat Anti-Mouse IFN‑beta Monoclonal Antibody (MAB82342) coated on a Clear Polystyrene Microplate (DY990). Rat Anti-Mouse IFN‑beta Monoclonal Antibody (Catalog # MAB82341) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Mouse IFN‑ beta Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse IFN‑ beta Monoclonal Antibody (Catalog # MAB82342).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IFN-beta DuoSet ELISA Kit (Catalog # DY8234-05) for convenient development of a sandwich ELISA or the Mouse IFN-beta Quantikine ELISA Kit (Catalog # MIFNB0) for  a complete optimized ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).

References

  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Teige, I. et al. (2003) J. Immunol. 170:4776.
  6. Shinohara, M.L. et al. (2008) Immunity 29:68.
  7. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  8. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  9. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  10. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
  11. Wang, H. et al. (2011) J. Immunol 186:675.

Long Name

Interferon beta

Alternate Names

IFB, IFF, IFNB, IFNB1, IFNbeta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Gene Symbol

IFNB1

UniProt

P01575

Additional IFN-beta Products

Product Documents for Mouse IFN‑ beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IFN‑ beta Antibody

For research use only

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