|Detection of IL‑10 R beta in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL‑10 R beta Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5368, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).|
|Detection of Mouse IL‑10 R beta by Western Blot. Western blot shows lysates of Differentiated 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL‑10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5368) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL‑10 R beta at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
IL-10 R beta (Interleukin 10 receptor beta; also IL‑10 R2, CD210b and CRF2-4) is a 60‑65 kDa member of the type II cytokine receptor family of proteins. It is very widely expressed, and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B and -29 (plus IL-26 in human). Mature mouse IL-10 R beta is a type I transmembrane protein that is 330 amino acids (aa) in length. It contains a 201 aa extracellular domain (aa 20‑220) that possesses two fibronectin type III domains (aa 22‑107 and 111‑208). There is one alternate start site at Met20. Over aa 21‑220, mouse IL-10 R beta shares 87% and 74% aa identity with rat and human IL-10 R beta, respectively.
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