Detects mouse IL-10 R beta in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) IL‑10 R beta is observed and less than 1% cross-reactivity with rhIL-10 R and recombinant mouse IL-10 R is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant mouse IL-10 R beta Ile21-Ser220 Accession # Q61190
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑10 R beta in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL‑10 R beta Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5368, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Detection of Mouse IL‑10 R beta by Western Blot. Western blot shows lysates of Differentiated 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL‑10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5368) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL‑10 R beta at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10 R beta
IL-10 R beta (Interleukin 10 receptor beta; also IL‑10 R2, CD210b and CRF2-4) is a 60‑65 kDa member of the type II cytokine receptor family of proteins. It is very widely expressed, and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B and -29 (plus IL-26 in human). Mature mouse IL-10 R beta is a type I transmembrane protein that is 330 amino acids (aa) in length. It contains a 201 aa extracellular domain (aa 20‑220) that possesses two fibronectin type III domains (aa 22‑107 and 111‑208). There is one alternate start site at Met20. Over aa 21‑220, mouse IL-10 R beta shares 87% and 74% aa identity with rat and human IL-10 R beta, respectively.
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