Mouse IL-11 Antibody

Catalog # Availability Size / Price Qty
MAB418
MAB418-SP
Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 Antibody.
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Product Details
Citations (3)
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Mouse IL-11 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 10‑20% cross-reactivity with recombinant human (rh) IL-11 is observed and no cross-reactivity with recombinant rat CNTF, recombinant mouse (rm) IL-6, rmLIF, rmOSM, rmCT-1, or rhCLC is observed.
Source
Monoclonal Rat IgG2A Clone # 188520
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-11
Gly23-Leu199
Accession # P47873
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑11 (Catalog # 418-ML)
Neutralization
Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse IL‑11.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 Antibody. View Larger

Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 Antibody. Recombinant Mouse IL‑11 (Catalog # 418-ML) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑11 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑11 Monoclonal Antibody (Catalog # MAB418). The ND50 is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-11

Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The mouse IL-11 cDNA encodes a 199 amino acid precursor polypeptide with a 22 amino acid hydrophobic signal that is processed proteolytically to generate the 177 amino acid mature protein. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cells. IL-11, in the presence of IL-3 or SCF, has also been shown to stimulate erythropoiesis. Among nonhematopoietic cell populations, IL-11, like IL-6 and LIF, can stimulate the synthesis of hepatic acute‑phase proteins. Consistent with the in vitro functions of IL‑11, in vivo administration of human IL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor alpha chain and gp130.

Long Name
Interleukin 11
Entrez Gene IDs
3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)
Alternate Names
Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin

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Citations for Mouse IL-11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. IL-11 Induces Encephalitogenic Th17 Cells in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis
    Authors: X Zhang, N Kiapour, S Kapoor, T Khan, M Thamilaras, Y Tao, S Cohen, R Miller, RA Sobel, S Markovic-P
    J. Immunol., 2019-07-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. IL-11 is required for A1 adenosine receptor-mediated protection against ischemic AKI.
    Authors: Kim J, Kim M, Ham A, Brown K, Greene R, D'Agati V, Lee H
    J Am Soc Nephrol, 2013-06-27;24(10):1558-70.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  3. Cold pre-conditioning neuroprotection depends on TNF-alpha and is enhanced by blockade of interleukin-11.
    Authors: Mitchell HM, White DM, Domowicz MS, Kraig RP
    J. Neurochem., 2010-12-01;117(2):187-96.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Neutralization

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