Detects mouse IL-11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 10‑20% cross-reactivity with recombinant human (rh) IL-11 is observed and no cross-reactivity with recombinant rat CNTF, recombinant mouse (rm) IL-6, rmLIF, rmOSM, rmCT-1, or rhCLC is observed.
Monoclonal Rat IgG2A Clone # 188520
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse IL-11 Gly23-Leu199 Accession # P47873
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse IL‑11.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 Antibody.
Recombinant Mouse IL‑11 (Catalog # 418-ML) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑11 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑11 Monoclonal Antibody (Catalog # MAB418). The ND50 is typically 0.5-2.0 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The mouse IL-11 cDNA encodes a 199 amino acid precursor polypeptide with a 22 amino acid hydrophobic signal that is processed proteolytically to generate the 177 amino acid mature protein. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cells. IL-11, in the presence of IL-3 or SCF, has also been shown to stimulate erythropoiesis. Among nonhematopoietic cell populations, IL-11, like IL-6 and LIF, can stimulate the synthesis of hepatic acute‑phase proteins. Consistent with the in vitro functions of IL‑11, in vivo administration of human IL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor alpha chain and gp130.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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