Mouse IL-12/IL-23 p40 Antibody

(8 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects the mouse IL-12/IL-23 p40 subunit. Polymorphisms exist in the mouse p40 sequences [Ymer, S. et al. (2002) Genes and Immunity 3:151.].
  • Source
    Monoclonal Rat IgG2A Clone # C17.8
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Recombinant mouse IL-12 p70 heterodimer
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse IL-12/IL-23 p40 Homodimer (Catalog # 499-ML)
    under non-reducing conditions only
    • Mouse IL-12/IL-23 p40 Nonallele-specific Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse IL‑12/IL‑23 p40 Antibody (Catalog # MAB4991)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse IL‑12/IL‑23 p40 Biotinylated Antibody (Catalog # BAF499)
  • ELISA Standard
     
    Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein (Catalog # 499-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated mouse splenocytes. The Neutralization Dose (ND50) is typically 0.04-0.3 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑12.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12/IL‑23 p40 Antibody. Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND50 is typically
0.04-0.3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12/IL-23 p40

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells.

IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells.

The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

  • Long Name:
    Interleukin 12/Interleukin 23 p40
  • Entrez Gene IDs:
    3593 (Human); 16160 (Mouse); 64546 (Rat); 403976 (Canine); 493741 (Feline)
  • Alternate Names:
    CLMF2; IL12 p40; IL-12 p40; IL12B; IL-12B; NKSF2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. IFN-?-Expressing Th17 Cells Are Required for Development of Severe Ocular Surface Autoimmunity
    Authors: Y Chen, SK Chauhan, C Shao, M Omoto, T Inomata, R Dana
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  2. M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
    Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
    Am J Pathol, 2015;185(2):420-31.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  3. Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens.
    Authors: DePaolo RW, Abadie V, Tang F
    Nature, 2011;471(7337):220-4.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. The pro-Th2 cytokine IL-33 directly interacts with invariant NKT and NK cells to induce IFN-gamma production.
    Authors: Bourgeois E, Van LP, Samson M, Diem S, Barra A, Roga S, Gombert JM, Schneider E, Dy M, Gourdy P, Girard JP, Herbelin A
    Eur. J. Immunol., 2009;39(4):1046-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  5. TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
    Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
    Cancer Res., 2008;68(23):9754-62.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
    Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
    J Ethnopharmacol, 2006;106(3):418-24.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  7. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004;104(5):1404-10.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  8. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.
    Authors: Oppmann, B, Lesley, R, Blom, B, Timans, J C, Xu, Y, Hunte, B, Vega, F, Yu, N, Wang, J, Singh, K, Zonin, F, Vaisberg, E, Churakova, T, Liu, M, Gorman, D, Wagner, J, Zurawski, S, Liu, Y, Abrams, J S, Moore, K W, Rennick, D, de Waal-Malefyt, R, Hannum, C, Bazan, J F, Kastelein, R A
    Immunity, 2000;13(5):715-25.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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