Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Avian - Duck

Applications

Validated:

Western Blot

Cited:

Western Blot, Neutralization, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 142206
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Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-2
Ala21-Gln169
Accession # P04351

Specificity

Detects mouse IL-2 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) IL‑2, rrIL‑2, rpIL‑2, rcrIL‑2, or rhIL‑15.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse IL-2 Antibody (142206)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑2 (Catalog # 402-ML)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2

IL-2 is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

UniProt

Additional IL-2 Products

Product Documents for Mouse IL-2 Antibody (142206)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-2 Antibody (142206)

For research use only

Citations for Mouse IL-2 Antibody (142206)

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FAQs

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