Mouse IL-2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF402
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse IL-2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-2 in ELISAs and Western blots. In western blots, less than 10% cross‑reactivity with rhIL‑2, rpIL-2 and rrIL‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-2 (R&D Systems, Catalog # 402-ML)
Ala21-Gln169
Accession # P04351
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑2 (Catalog # 402-ML)

Mouse IL-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IL‑2 Antibody (Catalog # MAB702)

Standard: Recombinant Mouse IL-2 Protein (Catalog # 402-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1 - 3). Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch (4). Mouse and human IL-2 exhibit cross-species activity (5). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (6 - 8). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (9). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (10 - 12). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (13, 14).

References
  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Kashima, N. et al. (1985) Nature 313:402.
  4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
  5. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  7. Wang, X. et al. (2005) Science 310:1159.
  8. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  9. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  10. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  11. Laurence, A. et al. (2007) Immunity 26:371.
  12. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  13. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  14. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Product Datasheets

Citations for Mouse IL-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex
    Authors: I Hwang, K Kim, S Choi, M Lomunova
    Mol. Cells, 2017;40(1):24-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development (Detection)
  2. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  3. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008;112(2):362-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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