Mouse IL-21 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC594P-100
IC594P-025
Detection of IL‑21 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (3)
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Mouse IL-21 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-21 in Western blots. Does not cross-react with recombinant human IL‑21, recombinant mouse (rm) IL‑15, or rmIL‑12.
Source
Monoclonal Rat IgG2B Clone # 149204
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL‑21
Pro25-Ser146
Accession # Q9ES17
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-21 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of IL‑21 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (light orange filled histogram) or treated with PMA and Calcium Ionomycin (dark orange filled histogram) were stained with Rat Anti-Mouse IL-21 PE-conjugated Monoclonal Antibody (Catalog # IC594P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-21

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most closely related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (Novel Interleukin Receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% aa identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appears to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

References
  1. Parrish-Novak, J. et al. (2000) Nature 408:57.
  2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21
Entrez Gene IDs
59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)
Alternate Names
CVID11; IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform; Za11

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Citations for Mouse IL-21 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. HSV-1 0?NLS vaccine elicits a robust B lymphocyte response and preserves vision without HSV-1 glycoprotein M or thymidine kinase recognition
    Authors: GB Gmyrek, AN Berube, VH Sjoelund, DJJ Carr
    Scientific Reports, 2022-09-23;12(1):15920.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Flow Cytometry
  2. Enhanced GITR/GITRL interactions augment IL-27 expression and induce IL-10-producing Tr-1 like cells.
    Authors: Carrier Y, Whitters MJ, Miyashiro JS, LaBranche TP, Ramon HE, Benoit SE, Ryan MS, Keegan SP, Guay H, Douhan J, Collins M, Dunussi-Joannopoulos K, Medley QG
    Eur. J. Immunol., 2012-06-01;42(6):1393-404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. ICOS expression by effector T cells influences the ability of regulatory T cells to inhibit anti-chromatin B cell responses in recipient mice.
    Authors: Hondowicz BD, Batheja AO, Metzgar MH
    J. Autoimmun., 2009-12-22;34(4):460-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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