Interleukin-22 (IL-22), also known as IL-10-related T cell-derived Inducible Factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
Discontinued Product
IC582F has been discontinued.
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Key Product Details
Species Reactivity
Mouse
Applications
Intracellular Staining by Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 140301
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Product Specifications
Immunogen
E. coli-derived recombinant mouse IL-22
Leu34-Val179
Accession # Q9JJY9
Leu34-Val179
Accession # Q9JJY9
Specificity
Detects mouse IL-22 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-22 is observed, 15% cross-reactivity with recombinant human IL-22 is observed, and no cross-reactivity with recombinant mouse IL-10 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse IL‑22 Fluorescein‑conjugated Antibody
Detection of IL‑22 in Th17 Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3 e monoclonal antibody (Catalog # MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (Catalog # 240-B, 10 ng/mL) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/mL), and Rat anti-Mouse IFN-? (Catalog # MAB485, 10 µg/mL) for 5 days were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (Catalog # FAB554A) and (A) Rat Anti-Mouse IL-22 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC582F) or (B) isotype control antibody (Catalog # IC006F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Mouse IL‑22 Fluorescein‑conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes treated with plate-bound Rat anti-Mouse CD3 epsilon monoclonal antibody (Catalog # MAB484, 5 μg/ml) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/ml), Recombinant Human TGF-beta 1 (Catalog # 240-B, 10 ng/ml) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/ml), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/ml), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/ml), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/ml) for 5 days were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (Catalog # FC012).
Sample: Mouse splenocytes treated with plate-bound Rat anti-Mouse CD3 epsilon monoclonal antibody (Catalog # MAB484, 5 μg/ml) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/ml), Recombinant Human TGF-beta 1 (Catalog # 240-B, 10 ng/ml) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/ml), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/ml), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/ml), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/ml) for 5 days were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (Catalog # FC012).
Reviewed Applications
Read 1 review rated 1 using IC582F in the following applications:
Spectra Viewer
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-22
References
- Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
- Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
- Dumoutier, L. et al. (2000) PNAS 97:10144.
- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Long Name
Interleukin 22
Alternate Names
IL-TIF, IL22
Gene Symbol
IL22
UniProt
Additional IL-22 Products
Product Documents for Mouse IL‑22 Fluorescein‑conjugated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IL‑22 Fluorescein‑conjugated Antibody
For research use only
Related Research Areas
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Application: Flow CytometrySample Tested: T cellsSpecies: MouseVerified Customer | Posted 12/23/2018Mouse splenic T cells were stimulated with anti-CD3, anti-CD28, IL-6, IL-1b, TGF-b, and IL-23 for 3 days.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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