Mouse IL-24 DuoSet ELISA
Mouse IL-24 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Data Example
Product Datasheets
Preparation and Storage
Background: IL-24
Interleukin 24 (IL-24), also known as MDA-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. When secreted, IL-24 is a 35- to 40-kDa phosphorylated glycoprotein that may exist as either a monomer or dimer. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. IL-24 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta.
Citations for Mouse IL-24 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Acute renal injury induced by endotoxic shock in rats is alleviated via PI3K/Nrf2 pathway
Authors: CH Chuang, CK Yang, PH Wu, Y Zhang, PJ Yang
Eur Rev Med Pharmacol Sci, 2018;22(16):5394-5401.
Species: Rat
Sample Types: Tissue Homogenates
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Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth
Authors: JC Klein, K Moses, G Zelinskyy, S Sody, J Buer, S Lang, I Helfrich, U Dittmer, CJ Kirschning, S Brandau
Nat Commun, 2017;8(0):14600.
Species: Mouse
Sample Types: Tissue Homogenates
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