Mouse IL-24 DuoSet ELISA

Catalog #: DY2786-05
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY2786-05

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DY008B: DuoSet ELISA Ancillary Reagent Kit 2

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Product Details

Citations (4)

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IL-24 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

7.8 - 500 pg/mL

Sufficient Materials

For five 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-24. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Mouse IL-24 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-24

Interleukin 24 (IL-24), also known as MDA-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. When secreted, IL-24 is a 35- to 40-kDa phosphorylated glycoprotein that may exist as either a monomer or dimer. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. IL-24 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta.

Long Name:

Interleukin 24

Entrez Gene IDs:

11009 (Human); 93672 (Mouse); 170819 (Rat)

Alternate Names:

C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein

Citations for Mouse IL-24 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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IL-20 Cytokines Are Involved in Epithelial Lesions Associated with Virus-Induced COPD Exacerbation in Mice
Authors: M Le Roux, A Ollivier, G Kervoaze, T Beke, L Gillet, M Pichavant, P Gosset
Biomedicines, 2021-12-05;9(12):.
Species: Mouse
Sample Types: BALF
Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation
Authors: JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng
Frontiers in Cellular Neuroscience, 2021-09-07;15(0):683687.
Species: Mouse
Sample Types: Tissue Homogenates
Acute renal injury induced by endotoxic shock in rats is alleviated via PI3K/Nrf2 pathway
Authors: CH Chuang, CK Yang, PH Wu, Y Zhang, PJ Yang
Eur Rev Med Pharmacol Sci, 2018-08-01;22(16):5394-5401.
Species: Rat
Sample Types: Tissue Homogenates
Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth
Authors: JC Klein, K Moses, G Zelinskyy, S Sody, J Buer, S Lang, I Helfrich, U Dittmer, CJ Kirschning, S Brandau
Nat Commun, 2017-03-16;8(0):14600.
Species: Mouse
Sample Types: Tissue Homogenates