Mouse IL-33 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF3626
Product Details
Citations (8)
FAQs
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Mouse IL-33 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-33 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant human IL‑33 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-33
Ser109-Ile266
Accession # Q8BVZ5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑33 (Catalog # 3626-ML)
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Mouse bEnd.3 endothelial cells, fixed with paraformaldehyde, and permeabilized with saponin

Mouse IL-33 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IL‑33 Antibody (Catalog # AF3626)

Standard: Recombinant Mouse IL-33 Protein (Catalog # 3626-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid (aa) sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

References
  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Long Name
Interleukin 33
Entrez Gene IDs
90865 (Human); 77125 (Mouse)
Alternate Names
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

Product Datasheets

Citations for Mouse IL-33 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  2. IL-33 is required for disposal of unnecessary cells during ovarian atresia through regulation of autophagy and macrophage migration.
    Authors: Wu J, Carlock C, Zhou C, Nakae S, Hicks J, Adams H, Lou Y
    J Immunol, 2015;194(5):2140-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
    Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
    Proc Natl Acad Sci U S A, 2015;112(22):E2891-9.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development (Detection)
  4. Severe neutrophil-dominated inflammation and enhanced myelopoiesis in IL-33-overexpressing CMV/IL33 mice.
    Authors: Talabot-Ayer D, Martin P, Vesin C, Seemayer C, Vigne S, Gabay C, Palmer G
    J Immunol, 2015;194(2):750-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Unique temporal and spatial expression patterns of IL-33 in ovaries during ovulation and estrous cycle are associated with ovarian tissue homeostasis.
    Authors: Carlock C, Wu J, Zhou C, Tatum K, Adams H, Tan F, Lou Y
    J Immunol, 2014;193(1):161-9.
  6. Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity.
    Authors: Palm N, Rosenstein R, Yu S, Schenten D, Florsheim E, Medzhitov R
    Immunity, 2013;39(5):976-85.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  7. Induction of IL-33 expression and activity in central nervous system glia.
    Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
    J. Leukoc. Biol., 2008;84(3):631-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  8. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 0;43(5):945-58.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)

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