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Mouse IL-33 Antibody

Catalog #: AF3626
76 Citations 5 Reviews Datasheet / COA / SDS
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AF3626
AF3626-SP

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Detection of Mouse IL‑33 by Western Blot.
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Citations (76)
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Mouse IL-33 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-33 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human IL‑33 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-33
Ser109-Ile266
Accession # Q8BVZ5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.4 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Mouse IL-33 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑33 Biotinylated Antibody (Catalog # BAF3626)

Standard: Recombinant Mouse IL-33 Protein (Catalog # 3626-ML)

Neutralization
Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 10-50 ng/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑33.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IL-33 antibody by Western Blot. View Larger

Detection of Mouse IL‑33 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with full length mouse IL-33. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IL-33 antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

IL‑33 in Mouse Spleen. IL-33 was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry IL-33 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

IL‑33 in bEnd.3 Mouse Cell Line. IL-33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of IL-33 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of IL‑33 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 5 hours with 50 ng/mL PMA and 1 µg/mL Ca2+ionomycin then stained with Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Neutralization Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody. View Larger

Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody. Recombinant Mouse IL-33 (Catalog # 3626-ML) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-33 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626). The ND50 is typically 10-50 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

References
  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Long Name
Interleukin 33
Entrez Gene IDs
90865 (Human); 77125 (Mouse)
Alternate Names
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

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Citations for Mouse IL-33 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

76 Citations: Showing 1 - 10
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  1. TMEM59 ablation leads to loss of olfactory sensory neurons and impairs olfactory functions via interaction with inflammation
    Authors: Z Ma, W Li, L Zhuang, T Wen, P Wang, H Yu, Y Liu, Y Yu
    Brain, Behavior, and Immunity, 2023-04-13;111(0):151-168.
    Species: Moyse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice
    Authors: M Liu, J Huang, T Liu, J Yuan, C Lv, Z Sha, C Wu, W Jiang, X Liu, M Nie, Y Chen, S Dong, Y Qian, C Gao, Y Fan, D Wu, R Jiang
    Acta neuropathologica communications, 2023-04-07;11(1):61.
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  3. Age-dependent Pdgfrbeta signaling drives adipocyte progenitor dysfunction to alter the beige adipogenic niche in male mice
    Authors: AM Benvie, D Lee, BM Steiner, S Xue, Y Jiang, DC Berry
    Nature Communications, 2023-03-31;14(1):1806.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Transcriptomic atlas and interaction networks of brain cells in mouse CNS demyelination and remyelination
    Authors: J Hou, Y Zhou, Z Cai, M Terekhova, A Swain, PS Andhey, RM Guimaraes, A Ulezko Ant, T Qiu, S Sviben, G Strout, JAJ Fitzpatric, Y Chen, S Gilfillan, DH Kim, SJ Van Dyken, MN Artyomov, M Colonna
    Cell Reports, 2023-03-21;42(4):112293.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. IL-33/ST2 axis promotes remodeling of the extracellular matrix and drives protective microglial responses in the mouse model of perioperative neurocognitive disorders
    Authors: S Li, H Liu, Y Qian, L Jiang, S Liu, Y Liu, C Liu, X Gu
    International immunopharmacology, 2022-11-26;114(0):109479.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  6. IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection
    Authors: L Xu, C Wei, Y Chen, Y Wu, X Shou, W Chen, D Lu, H Sun, W Li, B Yu, X Wang, X Zhang, Y Yu, Z Lei, R Tang, J Zhu, Y Li, L Lu, H Zhou, S Zhou, C Su, X Chen
    Nature Communications, 2022-11-12;13(1):6881.
    Species: Mouse
    Sample Types: In Vivo, Tissue Homogenates
    Applications: Neutralization, Western Blot
  7. Organoids capture tissue-specific innate lymphoid cell development in mice and humans
    Authors: GM Jowett, E Read, LB Roberts, D Coman, M Vilà Gonzá, T Zabinski, U Niazi, R Reis, TJ Trieu, D Danovi, E Gentleman, L Vallier, MA Curtis, GM Lord, JF Neves
    Cell Reports, 2022-08-30;40(9):111281.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. In mouse chronic pancreatitis CD25+FOXP3+ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response
    Authors: J Glaubitz, A Wilden, J Golchert, G Homuth, U Völker, BM Bröker, T Thiele, MM Lerch, J Mayerle, AA Aghdassi, FU Weiss, M Sendler
    Nature Communications, 2022-08-03;13(1):4502.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Short-term high fat feeding induces inflammatory responses of tuft cells and mucosal barrier cells in the murine stomach
    Authors: P Widmayer, P Pregitzer, H Breer
    Histology and histopathology, 2022-07-29;0(0):18503.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Tumor-educated Tregs drive organ-specific metastasis in breast cancer by impairing NK cells in the lymph node niche
    Authors: K Kos, MA Aslam, R van de Ven, MD Wellenstei, W Pieters, A van Weverw, DEM Duits, K van Pul, CS Hau, K Vrijland, D Kaldenbach, EAM Raeven, SA Quezada, R Beyaert, H Jacobs, TD de Gruijl, KE de Visser
    Cell Reports, 2022-03-01;38(9):110447.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. Therapeutic Potential of a Combination of Electroacupuncture and Human iPSC-Derived Small Extracellular Vesicles for Ischemic Stroke
    Authors: P Deng, L Wang, Q Zhang, S Chen, Y Zhang, H Xu, H Chen, Y Xu, W He, J Zhang, H Sun
    Cells, 2022-02-26;11(5):.
    Species: Mouse
    Sample Types: Tissue Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  12. Interleukin-33 activates group 2 innate lymphoid cell expansion and modulates endometriosis
    Authors: JE Miller, H Lingegowda, LK Symons, O Bougie, SL Young, BA Lessey, M Koti, C Tayade
    JCI Insight, 2021-12-08;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  13. Eosinophil extracellular traps drive asthma progression through neuro-immune signals
    Authors: Y Lu, Y Huang, J Li, J Huang, L Zhang, J Feng, J Li, Q Xia, Q Zhao, L Huang, S Jiang, S Su
    Nature Cell Biology, 2021-10-06;23(10):1060-1072.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung disease
    Authors: TR Mikuls, R Gaurav, GM Thiele, BR England, MG Wolfe, BP Shaw, KL Bailey, TA Wyatt, AJ Nelson, MJ Duryee, CD Hunter, D Wang, DJ Romberger, DP Ascherman, JA Poole
    International immunopharmacology, 2021-08-27;100(0):108069.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. A gene-environment-induced epigenetic program initiates tumorigenesis
    Authors: D Alonso-Cur, YJ Ho, C Burdziak, JLV Maag, JP Morris, R Chandwani, HA Chen, KM Tsanov, FM Barriga, W Luan, N Tasdemir, G Livshits, E Azizi, J Chun, JE Wilkinson, L Mazutis, SD Leach, R Koche, D Pe'er, SW Lowe
    Nature, 2021-02-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Heterogeneous bone-marrow stromal progenitors drive myelofibrosis via a druggable alarmin axis
    Authors: NB Leimkühler, HFE Gleitz, L Ronghui, IAM Snoeren, SNR Fuchs, JS Nagai, B Banjanin, KH Lam, T Vogl, C Kuppe, USA Stalmann, G Büsche, H Kreipe, I Gütgemann, P Krebs, Y Banz, P Boor, EW Tai, TH Brümmendor, S Koschmiede, M Crysandt, E Bindels, R Kramann, IG Costa, RK Schneider
    Cell Stem Cell, 2020-12-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M
    Authors: F Botelho, A Dubey, EA Ayaub, R Park, A Yip, A Humbles, R Kolbeck, CD Richards
    Mediators of Inflammation, 2020-11-28;2020(0):4087315.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  18. Hyperactivity of Innate Immunity Triggers Pain via TLR2-IL-33-Mediated Neuroimmune Crosstalk
    Authors: J Huang, MA Gandini, L Chen, S M'Dahoma, PL Stemkowski, H Chung, DA Muruve, GW Zamponi
    Cell Rep, 2020-10-06;33(1):108233.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Graft IL-33 regulates infiltrating macrophages to protect against chronic rejection
    Authors: T Li, Z Zhang, JG Bartolacci, GK Dwyer, Q Liu, L Mathews, M Velayutham, A Roessing, YC Lee, H Dai, S Shiva, MH Oberbarnsc, JL Dziki, SJ Mullett, SG Wendell, JD Wilkinson, SA Webber, MA Wood-Trage, SC Watkins, AJ Demetris, GS Hussey, SF Badylak, HR Turnquist
    J. Clin. Invest., 2020-10-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Fibroblast-derived IL-33 is dispensable for lymph node homeostasis but critical for CD8 T cell responses to acute and chronic viral infection
    Authors: P Aparicio-D, H Cannelle, MB Buechler, S Nguyen, SM Kallert, S Favre, N Alouche, N Papazian, B Ludewig, T Cupedo, DD Pinschewer, SJ Turley, SA Luther
    Eur. J. Immunol., 2020-08-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  21. Group 2 Innate Lymphoid Cells Must Partner with the Myeloid-Macrophage Lineage for Long-Term Postviral Lung Disease
    Authors: K Wu, X Wang, SP Keeler, BJ Gerovac, EV Agapov, DE Byers, S Gilfillan, M Colonna, Y Zhang, MJ Holtzman
    J. Immunol., 2020-07-08;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ICC, Western Blot
  22. IL-33/ST2 axis is involved in disease progression in the spleen during Leishmania donovani infection
    Authors: A Lamberet, O Rostan, S Dion, A Jan, H Guegan, C Manuel, M Samson, JP Gangneux, F Robert-Gan
    Parasit Vectors, 2020-06-22;13(1):320.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Strain-Specific Differences in House Dust Mite (Dermatophagoides farinae)-Induced Mouse Models of Allergic Rhinitis
    Authors: KI Lee, JS Bae, EH Kim, JH Kim, L Lyu, YJ Chung, JH Mo
    Clin Exp Otorhinolaryngol, 2020-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Cancer cell-derived interleukin-33 decoy receptor sST2 enhances orthotopic tumor growth in a murine pancreatic cancer model
    Authors: K Takenaga, M Akimoto, N Koshikawa, H Nagase
    PLoS ONE, 2020-04-27;15(4):e0232230.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity
    Authors: JA Moral, J Leung, LA Rojas, J Ruan, J Zhao, Z Sethna, A Ramnarain, B Gasmi, M Gururajan, D Redmond, G Askan, U Bhanot, E Elyada, Y Park, DA Tuveson, M Gönen, SD Leach, JD Wolchok, RP DeMatteo, T Merghoub, VP Balachandr
    Nature, 2020-02-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF, IHC
  26. Effective Nephroprotection Against Acute Kidney Injury with a Star-Shaped Polyglutamate-Curcuminoid Conjugate
    Authors: G Córdoba-Da, A Duro-Casta, RC Castelo-Br, C González-G, P Cannata, AB Sanz, MJ Vicent, A Ortiz, AM Ramos
    Sci Rep, 2020-02-06;10(1):2056.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  27. IL-33 deficiency causes persistent inflammation and severe neurodegeneration in retinal detachment
    Authors: J Augustine, S Pavlou, I Ali, K Harkin, E Ozaki, M Campbell, AW Stitt, H Xu, M Chen
    J Neuroinflammation, 2019-12-03;16(1):251.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. IL-33-mediated mast cell activation promotes gastric cancer through macrophage mobilization
    Authors: MF Eissmann, C Dijkstra, A Jarnicki, T Phesse, J Brunnberg, AR Poh, N Etemadi, E Tsantikos, S Thiem, ND Huntington, ML Hibbs, A Boussiouta, MA Grimbaldes, M Buchert, RJJ O'Donoghue, F Masson, M Ernst
    Nat Commun, 2019-06-21;10(1):2735.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  29. Sex differences in IL-17 contribute to chronicity in male versus female urinary tract infection
    Authors: A Zychlinsky, M Rousseau, L Lacerda Ma, T Canton, CR Consiglio, ML Albert, M Fontes, D Duffy, MA Ingersoll
    JCI Insight, 2019-05-30;5(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  30. Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway
    Authors: GS Hussey, JL Dziki, YC Lee, JG Bartolacci, M Behun, HR Turnquist, SF Badylak
    J Immunol Regen Med, 2019-02-01;3(0):26-35.
    Species: Mouse
    Sample Types: Matrix Bound Nanovesicles
    Applications: Western Blot
  31. IL-33 induction and signaling are controlled by glutaredoxin-1 in mouse macrophages
    Authors: EO Weinberg, B Ferran, Y Tsukahara, MMS Hatch, J Han, CE Murdoch, R Matsui
    PLoS ONE, 2019-01-25;14(1):e0210827.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. RIPK1 prevents TRADD-driven, but TNFR1 independent, apoptosis during development
    Authors: H Anderton, E Bandala-Sa, DS Simpson, JA Rickard, AP Ng, L Di Rago, C Hall, JE Vince, J Silke, G Liccardi, R Feltham
    Cell Death Differ., 2018-09-05;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  33. IL-1? mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation
    Authors: I Mahmutovic, M Menzel, S Ramu, S Cerps, H Akbarshahi, L Uller
    Respir. Res., 2018-01-24;19(1):16.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  34. Type 2 Cysteinyl Leukotriene Receptors Drive IL-33-Dependent Type 2 Immunopathology and Aspirin Sensitivity
    Authors: T Liu, NA Barrett, Y Kanaoka, E Yoshimoto, D Garofalo, H Cirka, C Feng, JA Boyce
    J. Immunol., 2017-12-27;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  35. Behavioral Changes in Mice Lacking Interleukin-33
    Authors: E Dohi, EY Choi, IVL Rose, AS Murata, S Chow, M Niwa, SI Kano
    eNeuro, 2017-12-26;4(6):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  36. Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms
    Authors: M Yamaguchi, SK Samuchiwal, O Quehenberg, JA Boyce, B Balestrier
    Mucosal Immunol, 2017-12-20;11(3):615-626.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  37. Cyclic stretch induced IL-33 production through HMGB1/TLR-4 signaling pathway in murine respiratory epithelial cells
    Authors: J Chang, Y Xia, K Wasserloos, M Deng, KJ Blose, DA Vorp, HR Turnquist, TR Billiar, BA Pitt, MZ Zhang, LM Zhang
    PLoS ONE, 2017-09-12;12(9):e0184770.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  39. IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model
    Authors: J Xu, J Guardado, R Hoffman, H Xu, R Namas, Y Vodovotz, L Xu, M Ramadan, J Brown, HR Turnquist, TR Billiar
    PLoS Med., 2017-07-25;14(7):e1002365.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  40. The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice
    Authors: M Han, C Rajput, JY Hong, J Lei, JL Hinde, Q Wu, JK Bentley, MB Hershenson
    J. Immunol., 2017-07-12;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  41. Early Postnatal Secondhand Smoke Exposure Disrupts Bacterial Clearance and Abolishes Immune Responses in Muco-Obstructive Lung Disease
    Authors: BW Lewis, R Sultana, R Sharma, A Noël, I Langohr, S Patial, AL Penn, Y Saini
    J. Immunol., 2017-06-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  42. Macrophages produce IL-33 by activating MAPK signaling pathway during RSV infection
    Authors: F Qi, S Bai, D Wang, L Xu, H Hu, S Zeng, R Chai, B Liu
    Mol. Immunol., 2017-05-19;87(0):284-292.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  43. IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis
    Authors: S Chen, B Chen, Z Wen, Z Huang, L Ye
    Oncotarget, 2017-05-16;8(20):32407-32418.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  44. Endogenous IL-33 Deficiency Exacerbates Liver Injury and Increases Hepatic Influx of Neutrophils in Acute Murine Viral Hepatitis
    Authors: V Carrière, MI Arshad, J Le Seyec, B Lefevre, M Farooq, A Jan, C Manuel, L Touami-Ber, C Lucas-Cler, V Genet, H Gascan, JP Girard, F Chalmel, L Lamontagne, C Piquet-Pel, M Samson
    Mediators Inflamm., 2017-05-04;2017(0):1359064.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  45. IL-33 receptor ST2 regulates the cognitive impairments associated with experimental cerebral malaria
    Authors: F Reverchon, S Mortaud, M Sivoyon, I Maillet, A Laugeray, J Palomo, C Montécot, A Herzine, S Meme, W Meme, F Erard, B Ryffel, A Menuet, VFJ Quesniaux
    PLoS Pathog., 2017-04-27;13(4):e1006322.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  46. Biliary epithelial injury-induced regenerative response by IL-33 promotes cholangiocarcinogenesis from peribiliary glands
    Authors: H Nakagawa, N Suzuki, Y Hirata, Y Hikiba, Y Hayakawa, H Kinoshita, S Ihara, K Uchino, Y Nishikawa, H Ijichi, M Otsuka, J Arita, Y Sakamoto, K Hasegawa, N Kokudo, K Tateishi, K Koike
    Proc. Natl. Acad. Sci. U.S.A., 2017-04-24;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  47. Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice
    Authors: T Sezaki, Y Hirata, T Hagiwara, YI Kawamura, T Okamura, R Takanashi, K Nakano, M Tamura-Nak, LC Burkly, T Dohi
    World J. Gastroenterol., 2017-04-07;23(13):2294-2307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017-04-06;8(4):e2735.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  49. A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract
    Authors: JE Oh, DS Oh, HE Jung, HK Lee
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  50. Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis
    PLoS ONE, 2016-12-19;11(12):e0168080.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  51. IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy
    Proc. Natl. Acad. Sci. U.S.A., 2016-11-07;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  52. Inflammation elevated IL-33 originating from the lung mediates inflammation in acute lung injury
    Authors: Shi-Hui Lin
    Clin. Immunol, 2016-10-28;0(0):.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC-P, In Vivo
  53. LIPOPOLYSACCHARIDE DOMAINS MODULATE UROVIRULENCE
    Infect Immun, 2016-10-17;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  54. Collagen-induced arthritis and imiquimod-induced psoriasis develop independently of interleukin-33
    Authors: Sara Khaleghparast Athari
    Arthritis Res Ther, 2016-06-18;18(1):143.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  55. Increased expression of upstream TH2-cytokines in a mouse model of viral-induced asthma exacerbation.
    Authors: Mahmutovic Persson I, Akbarshahi H, Menzel M, Brandelius A, Uller L
    J Transl Med, 2016-02-16;14(1):52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  56. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015-12-16;13(0):381.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  57. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 2015-11-17;43(5):945-58.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  58. Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway.
    Authors: Liu T, Kanaoka Y, Barrett N, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw T, Katz H, Boyce J
    J Immunol, 2015-09-04;195(8):3537-45.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  59. IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms.
    Authors: Mager L, Riether C, Schurch C, Banz Y, Wasmer M, Stuber R, Theocharides A, Li X, Xia Y, Saito H, Nakae S, Baerlocher G, Manz M, McCoy K, Macpherson A, Ochsenbein A, Beutler B, Krebs P
    J Clin Invest, 2015-05-26;125(7):2579-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  60. AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
    Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
    Proc Natl Acad Sci U S A, 2015-05-18;112(22):E2891-9.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development (Capture)
  61. MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease.
    Authors: Millar N, Gilchrist D, Akbar M, Reilly J, Kerr S, Campbell A, Murrell G, Liew F, Kurowska-Stolarska M, McInnes I
    Nat Commun, 2015-04-10;6(0):6774.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  62. Critical role of IL-33 receptor ST2 in experimental cerebral malaria development.
    Authors: Palomo J, Reverchon F, Piotet J, Besnard A, Couturier-Maillard A, Maillet I, Tefit M, Erard F, Mazier D, Ryffel B, Quesniaux V
    Eur J Immunol, 2015-03-20;45(5):1354-65.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  63. Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences.
    Authors: Sundnes O, Pietka W, Loos T, Sponheim J, Rankin A, Pflanz S, Bertelsen V, Sitek J, Hol J, Haraldsen G, Khnykin D
    J Invest Dermatol, 2015-03-04;135(7):1771-80.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  64. Alternaria-derived serine protease activity drives IL-33-mediated asthma exacerbations.
    Authors: Snelgrove R, Gregory L, Peiro T, Akthar S, Campbell G, Walker S, Lloyd C
    J Allergy Clin Immunol, 2014-03-15;134(3):583-592.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  65. Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity.
    Authors: Palm N, Rosenstein R, Yu S, Schenten D, Florsheim E, Medzhitov R
    Immunity, 2013-10-24;39(5):976-85.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Peritoneal Fluid
    Applications: ELISA Development (Capture)
  66. Airway epithelial cells initiate the allergen response through transglutaminase 2 by inducing IL-33 expression and a subsequent Th2 response.
    Authors: Oh, Keunhee, Seo, Myung Wo, Lee, Ga Young, Byoun, Ok-Jin, Kang, Hye-Ryun, Cho, Sang-Heo, Lee, Dong-Sup
    Respir Res, 2013-03-13;14(0):35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  67. Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis.
    Authors: Scalfone, Lisa K, Nel, Hendrik, Gagliardo, Lucille, Cameron, Jody L, Al-Shokri, Shaikha, Leifer, Cynthia, Fallon, Padraic, Appleton, Judith A
    Infect Immun, 2013-02-12;81(4):1354-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  68. Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Authors: Paulissen G, El Hour M, Rocks N, Gueders M, Bureau F, Foidart J, Lopez-Otin C, Noel A, Cataldo D
    J Immunol, 2012-09-07;189(8):4135-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  69. Transcriptional regulation of murine IL-33 by TLR and non-TLR agonists.
    Authors: Polumuri SK, Jayakar GG, Shirey KA, Roberts ZJ, Perkins DJ, Pitha PM, Vogel SN
    J. Immunol., 2012-05-25;189(1):50-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  70. The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation.
    Authors: Byrne SN, Beaugie C, O'Sullivan C, Leighton S, Halliday GM
    Am. J. Pathol., 2011-05-13;179(1):211-22.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC-Fr, Neutralization
  71. Paracrine IL-33 Stimulation Enhances Lipopolysaccharide-Mediated Macrophage Activation.
    Authors: Ohno T, Oboki K, Morita H
    PLoS ONE, 2011-04-11;6(4):e18404.
    Species: Mouse
    Sample Types: Recombinant Protein, Whole Cells
    Applications: ELISpot Development, Neutralization
  72. Mast cells as sensors of cell injury through IL-33 recognition.
    Authors: Enoksson M, Lyberg K, Moller-Westerberg C, Fallon PG, Nilsson G, Lunderius-Andersson C
    J. Immunol., 2011-01-14;186(4):2523-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  73. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina.
    Authors: Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C
    J. Immunol., 2011-01-10;186(4):2372-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  74. Caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages.
    Authors: Ohno T, Oboki K, Kajiwara N, Morii E, Aozasa K, Flavell RA, Okumura K, Saito H, Nakae S
    J. Immunol., 2009-12-15;183(12):7890-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Tissue Homogenates, Whole Cells
    Applications: ELISA Development, ELISpot Development, Western Blot
  75. IL-33 shifts macrophage polarization, promoting resistance against Pseudomonas aeruginosa keratitis.
    Authors: Hazlett LD, McClellan SA, Barrett RP, Huang X, Zhang Y, Wu M, Van Rooijen N, Szliter E
    Invest. Ophthalmol. Vis. Sci., 2009-11-05;51(3):1524-32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  76. Induction of IL-33 expression and activity in central nervous system glia.
    Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
    J. Leukoc. Biol., 2008-06-13;84(3):631-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Isotype Controls

Normal Goat IgG Control

AB-108-C
377 Citations
20 Reviews
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies

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Mouse IL-33 Antibody
By Sébastien Brot on 10/13/2023
Application: ICC/IF Sample Tested: Adult brain Species: Mouse

Expression of IL-33 (in red) in the mouse cortex, 7 days after traumatic brain injury

Immunocytochemistry/Immunofluorescence Mouse IL-33 Antibody AF3626
Mouse IL-33 Antibody
By Anonymous on 04/06/2021
Application: WB Sample Tested: Aorta tissue Species: Mouse
Western Blot Mouse IL-33 Antibody AF3626
Mouse IL-33 Antibody
By Josh Richards on 01/28/2020
Application: ELISA Sample Tested: Mouse lymphocytes Species: Mouse

Incubated with Protein X suspected of blocking activity of mIL-33

ELISA Mouse IL-33 Antibody AF3626
Mouse IL-33 Antibody
By Anonymous on 10/06/2019
Application: ICC/IF Sample Tested: Spleen tissue Species: Mouse

green: IL-33, red: vimentin

Immunocytochemistry/Immunofluorescence Mouse IL-33 Antibody AF3626
Mouse IL-33 Antibody
By Anonymous on 09/07/2017
Application: ELISA Sample Tested: Lung tissue Species: Mouse