Mouse IL-33 Antibody

(37 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-33 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human IL‑33 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IL-33
    Ser109-Ile266
    Accession # Q8BVZ5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.4 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
    • Mouse IL-33 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse IL‑33 Antibody (Catalog # AF3626)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse IL‑33 Biotinylated Antibody (Catalog # BAF3626)
  • ELISA Standard
     
    Recombinant Mouse IL-33 Protein (Catalog # 3626-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 10-50 ng/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑33.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IL‑33 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with full length mouse IL-33. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
IL‑33 in Mouse Spleen. IL‑33 was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Intracellular Staining by Flow Cytometry
Detection of IL‑33 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 5 hours with 50 ng/mL PMA and 1 μg/mL Ca2+ ionomycin then stained with Goat Anti-Mouse IL‑33 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3626, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Immunocytochemistry
IL‑33 in bEnd.3 Mouse Cell Line. IL‑33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse
IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody. Recombinant Mouse IL‑33 (Catalog #
3626-ML) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑33 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626). The ND50 is typically 10-50 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

  • References:
    1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
    2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
    3. Schmitz, J. et al. (2005) Immunity 23:479.
    4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
    5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
    6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
    7. Dinarello, C.A. (2005) Immunity 23:461.
    8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  • Long Name:
    Interleukin 33
  • Entrez Gene IDs:
    90865 (Human); 77125 (Mouse)
  • Alternate Names:
    C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Early Postnatal Secondhand Smoke Exposure Disrupts Bacterial Clearance and Abolishes Immune Responses in Muco-Obstructive Lung Disease
    Authors: BW Lewis, R Sultana, R Sharma, A Noël, I Langohr, S Patial, AL Penn, Y Saini
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model
    Authors: J Xu, J Guardado, R Hoffman, H Xu, R Namas, Y Vodovotz, L Xu, M Ramadan, J Brown, HR Turnquist, TR Billiar
    PLoS Med., 2017;14(7):e1002365.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract
    Authors: JE Oh, DS Oh, HE Jung, HK Lee
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  4. Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice
    Authors: T Sezaki, Y Hirata, T Hagiwara, YI Kawamura, T Okamura, R Takanashi, K Nakano, M Tamura-Nak, LC Burkly, T Dohi
    World J. Gastroenterol., 2017;23(13):2294-2307.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Biliary epithelial injury-induced regenerative response by IL-33 promotes cholangiocarcinogenesis from peribiliary glands
    Authors: H Nakagawa, N Suzuki, Y Hirata, Y Hikiba, Y Hayakawa, H Kinoshita, S Ihara, K Uchino, Y Nishikawa, H Ijichi, M Otsuka, J Arita, Y Sakamoto, K Hasegawa, N Kokudo, K Tateishi, K Koike
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017;8(4):e2735.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Capture
  8. IL-33 receptor ST2 regulates the cognitive impairments associated with experimental cerebral malaria
    Authors: F Reverchon, S Mortaud, M Sivoyon, I Maillet, A Laugeray, J Palomo, C Montécot, A Herzine, S Meme, W Meme, F Erard, B Ryffel, A Menuet, VFJ Quesniaux
    PLoS Pathog., 2017;13(4):e1006322.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  9. Endogenous IL-33 Deficiency Exacerbates Liver Injury and Increases Hepatic Influx of Neutrophils in Acute Murine Viral Hepatitis
    Authors: V Carrière, MI Arshad, J Le Seyec, B Lefevre, M Farooq, A Jan, C Manuel, L Touami-Ber, C Lucas-Cler, V Genet, H Gascan, JP Girard, F Chalmel, L Lamontagne, C Piquet-Pel, M Samson
    Mediators Inflamm., 2017;2017(0):1359064.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  10. IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis
    Authors: S Chen, B Chen, Z Wen, Z Huang, L Ye
    Oncotarget, 2017;8(20):32407-32418.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  11. The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice
    Authors: M Han, C Rajput, JY Hong, J Lei, JL Hinde, Q Wu, JK Bentley, MB Hershenson
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  12. LIPOPOLYSACCHARIDE DOMAINS MODULATE UROVIRULENCE
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  13. Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis
    PLoS ONE, 2016;11(12):e0168080.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  14. IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  15. Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway.
    Authors: Liu T, Kanaoka Y, Barrett N, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw T, Katz H, Boyce J
    J Immunol, 2016;195(8):3537-45.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  16. Collagen-induced arthritis and imiquimod-induced psoriasis develop independently of interleukin-33
    Authors: Sara Khaleghparast Athari
    Arthritis Res Ther, 2016;18(1):143.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  17. Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences.
    Authors: Sundnes O, Pietka W, Loos T, Sponheim J, Rankin A, Pflanz S, Bertelsen V, Sitek J, Hol J, Haraldsen G, Khnykin D
    J Invest Dermatol, 2015;135(7):1771-80.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  18. Critical role of IL-33 receptor ST2 in experimental cerebral malaria development.
    Authors: Palomo J, Reverchon F, Piotet J, Besnard A, Couturier-Maillard A, Maillet I, Tefit M, Erard F, Mazier D, Ryffel B, Quesniaux V
    Eur J Immunol, 2015;45(5):1354-65.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  19. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015;13(0):381.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  20. AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
    Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
    Proc Natl Acad Sci U S A, 2015;112(22):E2891-9.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Capture
  21. MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease.
    Authors: Millar N, Gilchrist D, Akbar M, Reilly J, Kerr S, Campbell A, Murrell G, Liew F, Kurowska-Stolarska M, McInnes I
    Nat Commun, 2015;6(0):6774.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  22. IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms.
    Authors: Mager L, Riether C, Schurch C, Banz Y, Wasmer M, Stuber R, Theocharides A, Li X, Xia Y, Saito H, Nakae S, Baerlocher G, Manz M, McCoy K, Macpherson A, Ochsenbein A, Beutler B, Krebs P
    J Clin Invest, 2015;125(7):2579-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  23. Increased expression of upstream TH2-cytokines in a mouse model of viral-induced asthma exacerbation.
    Authors: Mahmutovic Persson I, Akbarshahi H, Menzel M, Brandelius A, Uller L
    J Transl Med, 2015;14(1):52.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  24. Alternaria-derived serine protease activity drives IL-33-mediated asthma exacerbations.
    Authors: Snelgrove R, Gregory L, Peiro T, Akthar S, Campbell G, Walker S, Lloyd C
    J Allergy Clin Immunol, 2014;134(3):583-592.e6.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  25. Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis.
    Authors: Scalfone, Lisa K, Nel, Hendrik, Gagliardo, Lucille, Cameron, Jody L, Al-Shokri, Shaikha, Leifer, Cynthia, Fallon, Padraic, Appleton, Judith A
    Infect Immun, 2013;81(4):1354-63.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  26. Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity.
    Authors: Palm N, Rosenstein R, Yu S, Schenten D, Florsheim E, Medzhitov R
    Immunity, 2013;39(5):976-85.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  27. Airway epithelial cells initiate the allergen response through transglutaminase 2 by inducing IL-33 expression and a subsequent Th2 response.
    Authors: Oh, Keunhee, Seo, Myung Wo, Lee, Ga Young, Byoun, Ok-Jin, Kang, Hye-Ryun, Cho, Sang-Heo, Lee, Dong-Sup
    Respir Res, 2013;14(0):35.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  28. Transcriptional regulation of murine IL-33 by TLR and non-TLR agonists.
    Authors: Polumuri SK, Jayakar GG, Shirey KA, Roberts ZJ, Perkins DJ, Pitha PM, Vogel SN
    J. Immunol., 2012;189(1):50-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  29. Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Authors: Paulissen G, El Hour M, Rocks N, Gueders M, Bureau F, Foidart J, Lopez-Otin C, Noel A, Cataldo D
    J Immunol, 2012;189(8):4135-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  30. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina.
    Authors: Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C
    J. Immunol., 2011;186(4):2372-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  31. Paracrine IL-33 Stimulation Enhances Lipopolysaccharide-Mediated Macrophage Activation.
    Authors: Ohno T, Oboki K, Morita H
    PLoS ONE, 2011;6(4):e18404.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: Neut
  32. Mast cells as sensors of cell injury through IL-33 recognition.
    Authors: Enoksson M, Lyberg K, Moller-Westerberg C, Fallon PG, Nilsson G, Lunderius-Andersson C
    J. Immunol., 2011;186(4):2523-8.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  33. The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation.
    Authors: Byrne SN, Beaugie C, O'Sullivan C, Leighton S, Halliday GM
    Am. J. Pathol., 2011;179(1):211-22.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  34. IL-33 shifts macrophage polarization, promoting resistance against Pseudomonas aeruginosa keratitis.
    Authors: Hazlett LD, McClellan SA, Barrett RP, Huang X, Zhang Y, Wu M, Van Rooijen N, Szliter E
    Invest. Ophthalmol. Vis. Sci., 2010;51(3):1524-32.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  35. Caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages.
    Authors: Ohno T, Oboki K, Kajiwara N, Morii E, Aozasa K, Flavell RA, Okumura K, Saito H, Nakae S
    J. Immunol., 2009;183(12):7890-7.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  36. Induction of IL-33 expression and activity in central nervous system glia.
    Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
    J. Leukoc. Biol., 2008;84(3):631-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  37. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 0;43(5):945-58.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
Expand to show all 37 Citations
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Mouse IL-33 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Mouse IL-33 Antibody.

We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ELISA.

Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Mouse
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
 ELISA Mouse Anonymous 09/07/2017
Show

Summary

ApplicationELISA
Sample TestedLung tissue
SpeciesMouse

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Associated Pathways

Feedback

Customer Information