Mouse IL-33 Antibody

Catalog # Availability Size / Price Qty
AF3626
AF3626-SP
Detection of Mouse IL‑33 by Western Blot.
5 Images
Product Details
Citations (46)
FAQs
Supplemental Products
Reviews (1)

Mouse IL-33 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-33 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human IL‑33 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-33
Ser109-Ile266
Accession # Q8BVZ5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.4 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Mouse IL-33 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑33 Biotinylated Antibody (Catalog # BAF3626)

Standard: Recombinant Mouse IL-33 Protein (Catalog # 3626-ML)

Neutralization
Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 10-50 ng/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑33.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse IL‑33 by Western Blot. View Larger

Detection of Mouse IL‑33 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with full length mouse IL-33. PVDF membrane was probed with 0.4 µg/mL of Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IL‑33 in Mouse Spleen. View Larger

IL‑33 in Mouse Spleen. IL‑33 was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of IL‑33 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of IL‑33 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 5 hours with 50 ng/mL PMA and 1 μg/mL Ca2+ ionomycin then stained with Goat Anti-Mouse IL‑33 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3626, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunocytochemistry IL‑33 in bEnd.3 Mouse Cell Line. View Larger

IL‑33 in bEnd.3 Mouse Cell Line. IL‑33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse
IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Neutralization Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody. View Larger

Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody. Recombinant Mouse IL‑33 (Catalog #
3626-ML) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑33 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626). The ND50 is typically 10-50 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

References
  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Long Name
Interleukin 33
Entrez Gene IDs
90865 (Human); 77125 (Mouse)
Alternate Names
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

Product Datasheets

Citations for Mouse IL-33 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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  1. IL-33 induction and signaling are controlled by glutaredoxin-1 in mouse macrophages
    Authors: EO Weinberg, B Ferran, Y Tsukahara, MMS Hatch, J Han, CE Murdoch, R Matsui
    PLoS ONE, 2019;14(1):e0210827.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. RIPK1 prevents TRADD-driven, but TNFR1 independent, apoptosis during development
    Authors: H Anderton, E Bandala-Sa, DS Simpson, JA Rickard, AP Ng, L Di Rago, C Hall, JE Vince, J Silke, G Liccardi, R Feltham
    Cell Death Differ., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms
    Authors: M Yamaguchi, SK Samuchiwal, O Quehenberg, JA Boyce, B Balestrier
    Mucosal Immunol, 2018;11(3):615-626.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. IL-1? mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation
    Authors: I Mahmutovic, M Menzel, S Ramu, S Cerps, H Akbarshahi, L Uller
    Respir. Res., 2018;19(1):16.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. IL-33 receptor ST2 regulates the cognitive impairments associated with experimental cerebral malaria
    Authors: F Reverchon, S Mortaud, M Sivoyon, I Maillet, A Laugeray, J Palomo, C Montécot, A Herzine, S Meme, W Meme, F Erard, B Ryffel, A Menuet, VFJ Quesniaux
    PLoS Pathog., 2017;13(4):e1006322.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Early Postnatal Secondhand Smoke Exposure Disrupts Bacterial Clearance and Abolishes Immune Responses in Muco-Obstructive Lung Disease
    Authors: BW Lewis, R Sultana, R Sharma, A Noël, I Langohr, S Patial, AL Penn, Y Saini
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Endogenous IL-33 Deficiency Exacerbates Liver Injury and Increases Hepatic Influx of Neutrophils in Acute Murine Viral Hepatitis
    Authors: V Carrière, MI Arshad, J Le Seyec, B Lefevre, M Farooq, A Jan, C Manuel, L Touami-Ber, C Lucas-Cler, V Genet, H Gascan, JP Girard, F Chalmel, L Lamontagne, C Piquet-Pel, M Samson
    Mediators Inflamm., 2017;2017(0):1359064.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017;8(4):e2735.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice
    Authors: T Sezaki, Y Hirata, T Hagiwara, YI Kawamura, T Okamura, R Takanashi, K Nakano, M Tamura-Nak, LC Burkly, T Dohi
    World J. Gastroenterol., 2017;23(13):2294-2307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  11. Cyclic stretch induced IL-33 production through HMGB1/TLR-4 signaling pathway in murine respiratory epithelial cells
    Authors: J Chang, Y Xia, K Wasserloos, M Deng, KJ Blose, DA Vorp, HR Turnquist, TR Billiar, BA Pitt, MZ Zhang, LM Zhang
    PLoS ONE, 2017;12(9):e0184770.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Biliary epithelial injury-induced regenerative response by IL-33 promotes cholangiocarcinogenesis from peribiliary glands
    Authors: H Nakagawa, N Suzuki, Y Hirata, Y Hikiba, Y Hayakawa, H Kinoshita, S Ihara, K Uchino, Y Nishikawa, H Ijichi, M Otsuka, J Arita, Y Sakamoto, K Hasegawa, N Kokudo, K Tateishi, K Koike
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model
    Authors: J Xu, J Guardado, R Hoffman, H Xu, R Namas, Y Vodovotz, L Xu, M Ramadan, J Brown, HR Turnquist, TR Billiar
    PLoS Med., 2017;14(7):e1002365.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Macrophages produce IL-33 by activating MAPK signaling pathway during RSV infection
    Authors: F Qi, S Bai, D Wang, L Xu, H Hu, S Zeng, R Chai, B Liu
    Mol. Immunol., 2017;87(0):284-292.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Behavioral Changes in Mice Lacking Interleukin-33
    Authors: E Dohi, EY Choi, IVL Rose, AS Murata, S Chow, M Niwa, SI Kano
    eNeuro, 2017;4(6):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice
    Authors: M Han, C Rajput, JY Hong, J Lei, JL Hinde, Q Wu, JK Bentley, MB Hershenson
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis
    Authors: S Chen, B Chen, Z Wen, Z Huang, L Ye
    Oncotarget, 2017;8(20):32407-32418.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. Type 2 Cysteinyl Leukotriene Receptors Drive IL-33-Dependent Type 2 Immunopathology and Aspirin Sensitivity
    Authors: T Liu, NA Barrett, Y Kanaoka, E Yoshimoto, D Garofalo, H Cirka, C Feng, JA Boyce
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract
    Authors: JE Oh, DS Oh, HE Jung, HK Lee
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Inflammation elevated IL-33 originating from the lung mediates inflammation in acute lung injury
    Authors: Shi-Hui Lin
    Clin. Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  21. LIPOPOLYSACCHARIDE DOMAINS MODULATE UROVIRULENCE
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Collagen-induced arthritis and imiquimod-induced psoriasis develop independently of interleukin-33
    Authors: Sara Khaleghparast Athari
    Arthritis Res Ther, 2016;18(1):143.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  23. Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway.
    Authors: Liu T, Kanaoka Y, Barrett N, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw T, Katz H, Boyce J
    J Immunol, 2016;195(8):3537-45.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  24. Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis
    PLoS ONE, 2016;11(12):e0168080.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  26. Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences.
    Authors: Sundnes O, Pietka W, Loos T, Sponheim J, Rankin A, Pflanz S, Bertelsen V, Sitek J, Hol J, Haraldsen G, Khnykin D
    J Invest Dermatol, 2015;135(7):1771-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. Critical role of IL-33 receptor ST2 in experimental cerebral malaria development.
    Authors: Palomo J, Reverchon F, Piotet J, Besnard A, Couturier-Maillard A, Maillet I, Tefit M, Erard F, Mazier D, Ryffel B, Quesniaux V
    Eur J Immunol, 2015;45(5):1354-65.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
    Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
    Proc Natl Acad Sci U S A, 2015;112(22):E2891-9.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development (Capture)
  29. MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease.
    Authors: Millar N, Gilchrist D, Akbar M, Reilly J, Kerr S, Campbell A, Murrell G, Liew F, Kurowska-Stolarska M, McInnes I
    Nat Commun, 2015;6(0):6774.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  30. IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms.
    Authors: Mager L, Riether C, Schurch C, Banz Y, Wasmer M, Stuber R, Theocharides A, Li X, Xia Y, Saito H, Nakae S, Baerlocher G, Manz M, McCoy K, Macpherson A, Ochsenbein A, Beutler B, Krebs P
    J Clin Invest, 2015;125(7):2579-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  31. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015;13(0):381.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  32. Increased expression of upstream TH2-cytokines in a mouse model of viral-induced asthma exacerbation.
    Authors: Mahmutovic Persson I, Akbarshahi H, Menzel M, Brandelius A, Uller L
    J Transl Med, 2015;14(1):52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  33. Alternaria-derived serine protease activity drives IL-33-mediated asthma exacerbations.
    Authors: Snelgrove R, Gregory L, Peiro T, Akthar S, Campbell G, Walker S, Lloyd C
    J Allergy Clin Immunol, 2014;134(3):583-592.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  34. Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity.
    Authors: Palm N, Rosenstein R, Yu S, Schenten D, Florsheim E, Medzhitov R
    Immunity, 2013;39(5):976-85.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  35. Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis.
    Authors: Scalfone, Lisa K, Nel, Hendrik, Gagliardo, Lucille, Cameron, Jody L, Al-Shokri, Shaikha, Leifer, Cynthia, Fallon, Padraic, Appleton, Judith A
    Infect Immun, 2013;81(4):1354-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. Airway epithelial cells initiate the allergen response through transglutaminase 2 by inducing IL-33 expression and a subsequent Th2 response.
    Authors: Oh, Keunhee, Seo, Myung Wo, Lee, Ga Young, Byoun, Ok-Jin, Kang, Hye-Ryun, Cho, Sang-Heo, Lee, Dong-Sup
    Respir Res, 2013;14(0):35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Authors: Paulissen G, El Hour M, Rocks N, Gueders M, Bureau F, Foidart J, Lopez-Otin C, Noel A, Cataldo D
    J Immunol, 2012;189(8):4135-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  38. Transcriptional regulation of murine IL-33 by TLR and non-TLR agonists.
    Authors: Polumuri SK, Jayakar GG, Shirey KA, Roberts ZJ, Perkins DJ, Pitha PM, Vogel SN
    J. Immunol., 2012;189(1):50-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. Mast cells as sensors of cell injury through IL-33 recognition.
    Authors: Enoksson M, Lyberg K, Moller-Westerberg C, Fallon PG, Nilsson G, Lunderius-Andersson C
    J. Immunol., 2011;186(4):2523-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  40. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina.
    Authors: Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C
    J. Immunol., 2011;186(4):2372-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  41. Paracrine IL-33 Stimulation Enhances Lipopolysaccharide-Mediated Macrophage Activation.
    Authors: Ohno T, Oboki K, Morita H
    PLoS ONE, 2011;6(4):e18404.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Neutralization
  42. The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation.
    Authors: Byrne SN, Beaugie C, O'Sullivan C, Leighton S, Halliday GM
    Am. J. Pathol., 2011;179(1):211-22.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  43. IL-33 shifts macrophage polarization, promoting resistance against Pseudomonas aeruginosa keratitis.
    Authors: Hazlett LD, McClellan SA, Barrett RP, Huang X, Zhang Y, Wu M, Van Rooijen N, Szliter E
    Invest. Ophthalmol. Vis. Sci., 2010;51(3):1524-32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  44. Caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages.
    Authors: Ohno T, Oboki K, Kajiwara N, Morii E, Aozasa K, Flavell RA, Okumura K, Saito H, Nakae S
    J. Immunol., 2009;183(12):7890-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  45. Induction of IL-33 expression and activity in central nervous system glia.
    Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
    J. Leukoc. Biol., 2008;84(3):631-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  46. Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
    Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
    Immunity, 0;43(5):945-58.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)

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Mouse IL-33 Antibody
By Anonymous on 09/07/2017
Application: ELISA Sample Tested: Lung tissue Species: Mouse