Mouse IL-38/IL-1F10 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB77742-100
MAB77742-SP
Mouse  IL‑38/IL‑1F10 ELISA Standard Curve.
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Mouse IL-38/IL-1F10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-38/IL-1F10 in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG2A Clone # 798038R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-38/IL-1F10
Met1-Arg152
Accession # Q8R459
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog # MAB7774R).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IL-38/IL1F10 DuoSet ELISA Kit (Catalog # DY2427-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Mouse IL‑38/IL‑1F10 ELISA Standard Curve. Recombinant Mouse IL‑38/IL‑1F10 protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog # MAB77742) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog # MAB7774R) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: IL-38/IL-1F10

IL-1F10, also known as IL-1HY2, is a secreted protein that shares sequence and structural similarity with IL-1 receptor antagonist and other IL-1 family members. It is expressed in basal epithelia of the skin and in proliferating germinal center CD20+ B cells. IL-1F10 binds soluble IL-1RI in vitro and may participate in regulation of immune responses. Mouse IL-1F10 shares 82% and 93% aa identity with human and rat IL-1F10, respectively. IL-1F10 is predicted to be 17 kDa.

Long Name
Interleukin 1 Family Member 10
Entrez Gene IDs
84639 (Human); 215274 (Mouse)

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