Mouse IL-38/IL-1F10 Antibody Summary
Accession # Q8R459
This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog # MAB7774R).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IL-38/IL1F10 DuoSet ELISA Kit (Catalog # DY2427-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Mouse IL‑38/IL‑1F10 ELISA Standard Curve. Recombinant Mouse IL‑38/IL‑1F10 protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog # MAB77742) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog # MAB7774R) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
IL-1F10, also known as IL-1HY2, is a secreted protein that shares sequence and structural similarity with IL-1 receptor antagonist and other IL-1 family members. It is expressed in basal epithelia of the skin and in proliferating germinal center CD20+ B cells. IL-1F10 binds soluble IL-1RI in vitro and may participate in regulation of immune responses. Mouse IL-1F10 shares 82% and 93% aa identity with human and rat IL-1F10, respectively. IL-1F10 is predicted to be 17 kDa.
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