Human/Mouse IL-5 Antibody

(13 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
  • Source
    Monoclonal Rat IgG1 Clone # TRFK5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse T cell clone-derived partially purified recombinant mouse IL-5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-reported
    This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
    • Human IL-5 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Mouse IL‑5 Antibody (Catalog # MAB405)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human IL‑5 Biotinylated Antibody (Catalog # BAM6051)
  • ELISA Standard
     
    Recombinant Human IL-5 Protein (Catalog # 205-IL)
    • Mouse IL-5 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Mouse IL‑5 Antibody (Catalog # MAB405)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Mouse IL‑5 Biotinylated Antibody (Catalog # BAM705)
  • ELISA Standard
     
    Recombinant Mouse IL-5 Protein (Catalog # 405-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.004-0.015 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑5 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B) and Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB005). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Cell Proliferation Induced by IL‑5 and Neutralization by Human/Mouse IL‑5 Antibody. Recombinant Mouse IL‑5 (Catalog # 405-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405). The ND50 is typically
0.004-0.015 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

  • Long Name:
    Interleukin 5
  • Entrez Gene IDs:
    3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
  • Alternate Names:
    BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  2. Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice.
    Authors: Yamada T, Tani Y, Nakanishi H
    FASEB J., 2011;25(2):561-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. Evidence that 13-14 di-hydro, 15-keto prostaglandin D(2)-induced airway eosinophilia in guinea-pigs is independent of interleukin-5.
    Inflamm. Res., 2009;58(2):103-8.
    Species: Guinea Pig
    Sample Type: In Vivo
    Application: In vivo
  4. Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients' lungs through an allergen- and interleukin-5-dependent mechanism.
    Authors: Xavier-Elsas P, Santos-Maximiano E, Queto T, Mendonca-Sales S, Joseph D, Gaspar-Elsas MI, Vargaftig BB
    Clin. Exp. Allergy, 2007;37(1):29-38.
    Species: Mouse
    Sample Type: Peritoneal Fluid
    Application: ELISA Development
  5. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  6. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Type: Serum
    Application: Luminex Development
  7. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  8. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006;168(6):1940-50.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  9. The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
    Authors: Nygaard UC, Ormstad H, Aase A, Lovik M
    Toxicology, 2005;206(2):181-93.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  10. Arthropod-derived histamine-binding protein prevents murine allergic asthma.
    Authors: Couillin I, Maillet I, Jacobs M, Paesen GC, Moser R, Weston-Davies W
    J. Immunol., 2004;173(5):3281-6.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  11. Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
    Authors: Kobayashi T, Iijima K, Kita H
    J. Immunol., 2003;170(11):5756-63.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  12. Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
    Authors: Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J
    Pulm Pharmacol Ther, 2002;15(2):161-8.
    Species: Mouse
    Sample Type:
    Application: In vivo
  13. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002;160(4):1353-60.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
Expand to show all 13 Citations
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