Mouse IL-7 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB4071-100
MAB4071-SP
Cell Proliferation Induced by IL-7 and Neutralization by Mouse IL-7 Antibody.
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Mouse IL-7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-7 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2304B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-7
Glu26-Ile154
Accession # P10168
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). The Neutralization Dose (ND50) is typically 0.5-3 μg/mL in the presence of 1.5 ng/mL Recombinant Mouse IL‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by IL-7 and Neutralization by Mouse IL-7 Antibody. View Larger

Cell Proliferation Induced by IL-7 and Neutralization by Mouse IL-7 Antibody. Recombinant Mouse IL-7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse IL-7 Monoclonal Antibody (Catalog # MAB4071). The ND50 is typically 0.5-3 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7, previously known as pre-B-cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B‑cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4 + and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumorocidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.

Mouse IL-7 cDNA encodes a precursor protein of 154 amino residues containing a 25 amino acid residue signal peptide. Human IL-7 has approximately 65% amino acid sequence identity with mouse IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivities are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma  chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.

Long Name
Interleukin 7
Entrez Gene IDs
3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

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