Mouse Leptin R Antibody

(8 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Leptin R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
    Ala20-Gly839
    Accession # Q3US58
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR)
  • Flow Cytometry
    2.5 µg/106 cells
    Mouse splenocytes
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Leptin R in Rat Brain.

Leptin R was detected in perfusion fixed frozen sections of rat brain using 15 µg/mL Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the choroid plexus. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

  • References:
    1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
    2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
    3. Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
    4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
    5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.
  • Long Name:
    Leptin Receptor
  • Entrez Gene IDs:
    3953 (Human); 16847 (Mouse)
  • Alternate Names:
    B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. A dangerous liaison: Leptin and sPLA2-IIA join forces to induce proliferation and migration of astrocytoma cells
    Authors: R Martín, C Cordova, B Gutiérrez, M Hernández, ML Nieto
    PLoS ONE, 2017;12(3):e0170675.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Differential cytokine contributions of perivascular haematopoietic stem cell niches
    Authors: N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette
    Nat. Cell Biol, 2017;19(3):214-223.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Hypomorphism for RPGRIP1L, a ciliary gene vicinal to the FTO locus, causes increased adiposity in mice.
    Authors: Stratigopoulos G, Martin Carli J, O'Day D, Wang L, Leduc C, Lanzano P, Chung W, Rosenbaum M, Egli D, Doherty D, Leibel R
    Cell Metab, 2014;19(5):767-79.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  4. Kinetics of leptin binding to the Q223R leptin receptor.
    Authors: Verkerke H, Naylor C, Zabeau L, Tavernier J, Petri W, Marie C
    PLoS ONE, 2014;9(4):e94843.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  5. Deletion of selenoprotein M leads to obesity without cognitive deficits.
    Authors: Pitts, Matthew, Reeves, Mariclai, Hashimoto, Ann C, Ogawa, Ashley, Kremer, Penny, Seale, Lucia A, Berry, Marla J
    J Biol Chem, 2013;288(36):26121-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. Leptin enhances CC-chemokine ligand expression in cultured murine macrophage.
    Authors: Kiguchi N, Maeda T, Kobayashi Y, Fukazawa Y, Kishioka S
    Biochem. Biophys. Res. Commun., 2009;384(3):311-5.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  7. Soluble receptor inhibits leptin transport.
    Authors: Tu H, Kastin AJ, Hsuchou H, Pan W
    J. Cell. Physiol., 2008;214(2):301-5.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  8. High circulating leptin receptors with normal leptin sensitivity in liver-specific insulin receptor knock-out (LIRKO) mice.
    Authors: Cohen SE, Kokkotou E, Biddinger SB, Kondo T, Gebhardt R, Kratzsch J, Mantzoros CS, Kahn CR
    J. Biol. Chem., 2007;282(32):23672-8.
    Species: Mouse
    Sample Type: Serum
    Application: WB
Cell and Tissue Staining Kits
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Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Goat.
We have 1 review tested in 1 application: Flow Cytometry.

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 Flow Goat Anonymous 04/21/2016
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Summary

ApplicationFlow Cytometry
Sample TestedBone marrow cells
SpeciesGoat

Other Experimental Details

Other Experimental Details0.2ug/ml/10^6 cells

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