Mouse Meteorin-like/METRNL DuoSet ELISA

Catalog # Availability Size / Price Qty
DY6679
Ancillary Products Available
Mouse Meteorin-like / METRNL ELISA Standard Curve
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Mouse Meteorin-like/METRNL DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Meteorin-like / METRNL. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Mouse Meteorin-like / METRNL ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Meteorin-like/METRNL

METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. The name meteorin derives from the ability of the founding family member meteorin to transform glia into cells containing an extensive, meteor-like tail. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. Mature human METRNL is 266 amino acids (aa) in length (aa 46-311). It reportedly contains a signal sequence (aa 1-45), which suggests it may be secreted. Mature human and mouse METRNL share 79% aa sequence identity.

Entrez Gene IDs:
284207 (Human); 210029 (Mouse); 316842 (Rat)
Alternate Names:
Meteorin like; meteorin, glial cell differentiation regulator-like; meteorin-like protein; Meteorin-like; METRNL; MGC99788

Citations for Mouse Meteorin-like/METRNL DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Aerobic Exercise Increases Meteorin-Like Protein in Muscle and Adipose Tissue of Chronic High-Fat Diet-Induced Obese Mice
    Authors: JY Bae
    Biomed Res Int, 2018;2018(0):6283932.
    Species: Mouse
    Sample Types: Plasma
  2. Effects of detraining and retraining on muscle energy-sensing network and meteorin-like levels in obese mice
    Authors: JY Bae, J Woo, S Kang, KO Shin
    Lipids Health Dis, 2018;17(1):97.
    Species: Mouse
    Sample Types: Plasma
  3. Adipocyte Metrnl Antagonizes Insulin Resistance Through PPARgamma Signaling.
    Authors: Li Z, Song J, Zheng S, Fan M, Guan Y, Qu Y, Xu J, Wang P, Miao C
    Diabetes, 2015;64(12):4011-22.
    Species: Mouse
    Sample Types: Serum
  4. METEORIN-LIKE is a cytokine associated with barrier tissues and alternatively activated macrophages.
    Authors: Ushach I, Burkhardt A, Martinez C, Hevezi P, Gerber P, Buhren B, Schrumpf H, Valle-Rios R, Vazquez M, Homey B, Zlotnik A
    Clin Immunol, 2014;156(2):119-127.
    Species: Mouse
    Sample Types: Cell Culture Supernates

FAQs

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Reviews for Mouse Meteorin-like/METRNL DuoSet ELISA

Average Rating: 4 (Based on 1 Review)

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Mouse Meteorin-like/METRNL DuoSet ELISA
By Anonymous on 11/08/2018
Sample Tested: Serum