Mouse Meteorin-like/METRNL DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Mouse Meteorin-like / METRNL ELISA Standard Curve
Preparation and Storage
METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. The name meteorin derives from the ability of the founding family member meteorin to transform glia into cells containing an extensive, meteor-like tail. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. Mature human METRNL is 266 amino acids (aa) in length (aa 46-311). It reportedly contains a signal sequence (aa 1-45), which suggests it may be secreted. Mature human and mouse METRNL share 79% aa sequence identity.
Citations for Mouse Meteorin-like/METRNL DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Aerobic Exercise Increases Meteorin-Like Protein in Muscle and Adipose Tissue of Chronic High-Fat Diet-Induced Obese Mice
Authors: JY Bae
Biomed Res Int, 2018;2018(0):6283932.
Sample Types: Plasma
Effects of detraining and retraining on muscle energy-sensing network and meteorin-like levels in obese mice
Authors: JY Bae, J Woo, S Kang, KO Shin
Lipids Health Dis, 2018;17(1):97.
Sample Types: Plasma
Adipocyte Metrnl Antagonizes Insulin Resistance Through PPARgamma Signaling.
Authors: Li Z, Song J, Zheng S, Fan M, Guan Y, Qu Y, Xu J, Wang P, Miao C
Sample Types: Serum
METEORIN-LIKE is a cytokine associated with barrier tissues and alternatively activated macrophages.
Authors: Ushach I, Burkhardt A, Martinez C, Hevezi P, Gerber P, Buhren B, Schrumpf H, Valle-Rios R, Vazquez M, Homey B, Zlotnik A
Clin Immunol, 2014;156(2):119-127.
Sample Types: Cell Culture Supernates
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