Mouse OSM R beta Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse OSM R beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CLC, rhCNTF, rhOSM, recombinant mouse (rm) CT-1, rmIL-6, rmIL-11, or rmLIF is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 118125
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse OSM R beta
    Glu24-Leu738
    Accession # O70458
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse OSM R beta Fc Chimera (Catalog # 662-OR)
  • Flow Cytometry
    2.5 µg/106 cells
    D3 mouse embryonic stem cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
OSM R beta in D3 Mouse Cell Line. OSM R beta was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse OSM R beta Monoclonal Antibody (Catalog # MAB662) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OSM R beta

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) type I transmembrane protein which contains a 23 aa signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.

  • References:
    1. Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
    2. Tanaka, M. et al. (1999) Blood 93:804.
  • Long Name:
    Oncostatin M Receptor beta
  • Entrez Gene IDs:
    9180 (Human); 18414 (Mouse); 310132 (Rat)
  • Alternate Names:
    OSM R beta; OSMR; OSMRB
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Oncostatin M induces dendritic cell maturation and Th1 polarization.
    Authors: Jung ID, Noh KT, Lee CM, Chun SH, Jeong SK, Park JW, Park WS, Kim HW, Yun CH, Shin YK, Park YM
    Biochem. Biophys. Res. Commun., 2010;394(2):272-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Characterization of the potential subpopulation of bone marrow cells involved in the repair of injured liver tissue.
    Authors: Khurana S, Mukhopadhyay A
    Stem Cells, 2007;25(6):1439-47.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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