Detects mouse OSM R beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CLC, rhCNTF, rhOSM, recombinant mouse (rm) CT-1, rmIL-6, rmIL-11, or rmLIF is observed.
Monoclonal Rat IgG2A Clone # 118125
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse OSM R beta Glu24-Leu738 Accession # O70458
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
OSM R beta in D3 Mouse Cell Line.
OSM R beta was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse OSM R beta Monoclonal Antibody (Catalog # MAB662) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OSM R beta
Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) type I transmembrane protein which contains a 23 aa signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.
Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
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