Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell-association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The carboxy-terminal of OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor-related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF-kappaB ligand) and TRAIL (TNF-related apoptosis-inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1 - 4 (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.
Mouse Osteoprotegerin/TNFRSF11B Antibody
R&D Systems | Catalog # MAB4591
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Western Blot, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 110511
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteoprotegerin/TNFRSF11B
Gln21-Leu401 (Gln138Arg)
Accession # Q6PI12
Gln21-Leu401 (Gln138Arg)
Accession # Q6PI12
Specificity
Detects mouse Osteoprotegerin/TNFRSF11B in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant human (rh) Osteoprotegerin and no cross-reactivity with rh4-1BB, recombinant mouse (rm) CD27, rmCD40, rhDR3, rhDR6, rmFas, rmGITR, rhHVEM, rhLT beta R, rmRANK, rmTNF RI, or rmTNF RII.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Osteoprotegerin/TNFRSF11B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera (Catalog # 459-MO)
under non-reducing conditions only
Sample: Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera (Catalog # 459-MO)
under non-reducing conditions only
Mouse Osteoprotegerin/TNFRSF11B Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB4591 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Osteoprotegerin/TNFRSF11B
References
- Lacey, D.L. et al. (1998) Cell 93:165.
- Emery, J.G. et al. (1998) J. Biol. Chem. 273:14363.
- Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3597.
Alternate Names
OCIF, TNFRSF11B
Gene Symbol
TNFRSF11B
UniProt
Additional Osteoprotegerin/TNFRSF11B Products
Product Documents for Mouse Osteoprotegerin/TNFRSF11B Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Osteoprotegerin/TNFRSF11B Antibody
For research use only
Related Research Areas
Citations for Mouse Osteoprotegerin/TNFRSF11B Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
