Mouse Plexin B2 Antibody

Catalog # Availability Size / Price Qty
AF6836
AF6836-SP
Detection of Mouse Plexin B2 by Western Blot.
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Mouse Plexin B2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Plexin B2 in direct ELISAs and Western blots. In direct ELISAs, approximately 9% cross-reactivity with recombinant human Plexin B2 is observed, and less than 1% cross-reactivity with recombinant mouse Plexin B3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin B2
Leu20-Trp1029
Accession # B2RXS4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
0.6-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse Plexin B2 by Western Blot. View Larger

Detection of Mouse Plexin B2 by Western Blot. Western blot shows lysates of M1 mouse myeloid leukemia cell line, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Plexin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6836) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Plexin B2 at approximately 240 and 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Plexin B2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Plexin B2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse Plexin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6836, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Immunohistochemistry Plexin B2  in Mouse Embryo. View Larger

Plexin B2 in Mouse Embryo. Plexin B2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Plexin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6836) at 0.6 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin B2

Plexin B2 is a 240 kDa type I transmembrane (TM) glycoprotein of the Plexin B family of semaphorin receptors (1, 2). The mouse Plexin B2 cDNA encodes 1842 amino acids (aa) that include a 19 aa signal sequence, a 1182 aa extracellular domain (ECD), a 21 aa TM domain, and a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20‑468) and three IPT repeats (aa 806‑1096). The ECD may be cleaved into two subunits, a 170 kDa alpha ‑chain (aa 20‑1168) and an 80 kDa TM beta ‑chain, that remain noncovalently linked (1). Multiple splice variants may exist. Within aa 20‑1029 in the ECD, mouse Plexin B2 shares 82%, 93%, 80% and 79% aa identity with human, rat, canine and bovine Plexin B2, respectively. The B Plexins (B1, B2 and B3) share approximately 40% aa identity with each other. Plexin B2 mRNA is expressed in proliferating cerebellar granule cell progenitors, neuroepithelium, developing neurons, growth plate chondrocytes, tooth bud inner enamel epithelium, glomeruli and mesenchyme of the developing kidney, and in germinal center B lymphocytes when T cell help is present (3‑7). Plexin B2 is often co‑expressed with Plexin B1, and the two may form heterodimers (1, 4, 6). Genetic deletion of mouse Plexin B2 results in defects in proliferation and migration of cerebellar granule cells, abnormal development of the neural tube and disorganization of the embryonic brain; these defects are not seen when Plexin B1 is deleted (8-10). In adults, Plexin B2 is expressed in specialized vascular endothelia, pancreatic islets of Langerhans, and adrenal glands (11). Plexin B2 serves as a receptor for type 4 semaphorins, especially Sema4C and Sema4G (8‑12). B Plexins, including Plexin B2, can bind the scatter factor receptors, Met and Ron, and activate them upon semaphorin engagement (1, 13).

References
  1. Artigiani, S. et al. (2003) J. Biol. Chem. 278:10094.
  2. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  3. Friedel, R.H. et al. (2007) J. Neurosci. 27:3921.
  4. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  5. Zhang, M. et al. (2008) Bone 43:511.
  6. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
  7. Yu, D. et al. (2008) Immunol. Cell Biol. 86:3.
  8. Deng, S. et al. (2007) J. Neurosci. 27:6333.
  9. Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
  10. Maier, V. et al. (2011) Mol. Cell. Neurosci. 46:419.
  11. Zielonka, M. et al. (2010) Exp. Cell Res. 316:2477.
  12. Yukawa, K. et al. (2010) Int. J. Mol. Med. 25:225.
  13. Conrotto, P. et al. (2004) Oncogene 23:5131.
Entrez Gene IDs
23654 (Human); 140570 (Mouse); 315217 (Rat)
Alternate Names
MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; Plexin B2; plexin-B2; PLXNB2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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