Mouse Progranulin/PGRN Antibody

Name: Mouse Progranulin/PGRN Antibody
Brand: R&D Systems
Rating: 5 (1 reviews)

(8 citations)

(1 Review)

Western Blot

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Immunohistochemistry

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Species Reactivity
Mouse
Specificity
Detects mouse Progranulin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Progranulin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Progranulin
Thr18-Leu589
Accession # P28798
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended
Concentration

Sample

Western Blot

0.5 µg/mL

See below
Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Mouse Progranulin/PGRN by Western Blot. Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85‑90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Progranulin in Mouse Kidney. Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progranulin/PGRN

Mouse Progranulin, also known as Proepithelin, PC Cell-derived Growth Factor and Acrogranin, is a secreted protein of 572 amino acid residues. It contains seven and one half granulin repeats which are characterized by 6 disulfide bridges. Progranulin can be converted in vivo to granulins by proteolysis. Progranulin is a mitogen that stimulates proliferation and survival of various epithelial, mesenchymal and tumor cells. Several granulins have also been shown to be biologically active and may play a role in inflammation and wound healing. Mouse Progranulin shares 86% and 75% amino acid sequence homology with rat and human Progranulin, respectively.

Entrez Gene IDs:
2896 (Human); 14824 (Mouse)
Alternate Names:
Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species	Applications	Sample Type
Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease Authors: JM Van Kampen, DG Kay PLoS ONE, 2017;12(8):e0182896. Species: Mouse Sample Type: Tissue Homogenates Application: WB Lysosomal processing of progranulin Authors: X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu Mol Neurodegener, 2017;12(1):62. Species: Mouse Sample Type: Cell Lysates Application: WB Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina Authors: Y Kuse, K Tsuruma, S Sugitani, H Izawa, Y Ohno, M Shimazawa, H Hara Sci Rep, 2016;6(0):23811. Species: Mouse Sample Type: Cell Lysates Application: WB Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells. Authors: Luo L, Lu L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J Mol Med Rep, 2014;9(5):1675-80. Species: Mouse Sample Type: Cell Lysates Application: WB Progranulin regulates neuronal outgrowth independent of sortilin. Authors: Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L Mol Neurodegener, 2012;7(0):33. Species: Mouse Sample Type: Cell Lysate Application: WB p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts. Biochem Biophys Res Commun, 2012;430(1):419-24. Species: Mouse Sample Type: Cell Lysates Application: WB Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins. Authors: Almeida S, Zhou L, Gao FB PLoS ONE, 2011;6(10):e26454. Species: Mouse Sample Type: Whole Cells Application: ICC Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models. Authors: Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S J. Pathol., 2009;219(2):173-81. Species: Mouse Sample Type: Tissue Homogenates Application: WB

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Cell and Tissue Staining Kits

Description Application Cat# Citations Images

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1

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Mouse Progranulin/PGRN Antibody

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

NorthernLights Guard Mounting Media

VisUCyte Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Universal

VisUCyte Antigen Retrieval Reagent-Basic

Normal Sheep IgG Control

Sheep IgG HRP-conjugated Antibody

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Donkey Anti-Sheep IgG Biotinylated Antibody

Sheep IgG (H+L) PE-conjugated Antibody

Sheep IgG (H+L) APC-conjugated Antibody

Sheep IgG VisUCyte HRP Polymer Antibody

Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Donkey Anti-Sheep IgG (H+L) Affinity Purified PAb, XAbsorbed

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