Mouse Progranulin/PGRN Antibody

Catalog # Availability Size / Price Qty
AF2557
AF2557-SP
Detection of Mouse Progranulin/PGRN by Western Blot.
3 Images
Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (6)

Mouse Progranulin/PGRN Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Progranulin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Progranulin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Progranulin
Thr18-Leu589
Accession # P28798
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Progranulin/PGRN antibody by Western Blot. View Larger

Detection of Mouse Progranulin/PGRN by Western Blot. Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Progranulin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Progranulin in Mouse Kidney. Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry Progranulin/PGRN antibody in Mouse Splencytes by Immunocytochemistry (ICC). View Larger

Progranulin/PGRN in Mouse Splencytes. Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progranulin/PGRN

Mouse Progranulin, also known as Proepithelin, PC Cell-derived Growth Factor and Acrogranin, is a secreted protein of 572 amino acid residues. It contains seven and one half granulin repeats which are characterized by 6 disulfide bridges. Progranulin can be converted in vivo to granulins by proteolysis. Progranulin is a mitogen that stimulates proliferation and survival of various epithelial, mesenchymal and tumor cells. Several granulins have also been shown to be biologically active and may play a role in inflammation and wound healing. Mouse Progranulin shares 86% and 75% amino acid sequence homology with rat and human Progranulin, respectively.

Entrez Gene IDs
2896 (Human); 14824 (Mouse)
Alternate Names
Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin

Product Datasheets

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Citations for Mouse Progranulin/PGRN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Decreased Prosaposin and Progranulin in the Cingulate Cortex Are Associated with Schizophrenia Pathophysiology
    Authors: Y He, X Zhang, I Flais, P Svenningss
    International Journal of Molecular Sciences, 2022;23(19):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Progranulin Preserves Autophagy Flux and Mitochondrial Function in Rat Cortical Neurons Under High Glucose Stress
    Authors: C Dedert, V Mishra, G Aggarwal, AD Nguyen, F Xu
    Frontiers in Cellular Neuroscience, 2022;16(0):874258.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis
    Authors: MN Santos, DH Paushter, T Zhang, X Wu, T Feng, J Lou, H Du, SM Becker, R Fragoza, H Yu, F Hu
    The Journal of Biological Chemistry, 2022;0(0):102348.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Differential regulation of progranulin derived granulin peptides
    Authors: T Zhang, H Du, MN Santos, X Wu, MD Pagan, LJ Trigiani, N Nishimura, T Reinheckel, F Hu
    Molecular Neurodegeneration, 2022;17(1):15.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice
    Authors: M Murakoshi, T Gohda, E Adachi, S Ichikawa, S Hagiwara, Y Suzuki
    Scientific Reports, 2021;11(1):1194.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation
    Authors: K Takahashi, S Nakamura, W Otsu, M Shimazawa, H Hara
    Journal of Neuroinflammation, 2021;18(1):164.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
    Authors: J Frew, HB Nygaard
    Acta neuropathologica communications, 2021;9(1):57.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Effects of progranulin on the pathological conditions in experimental myocardial infarction model
    Authors: T Sasaki, M Shimazawa, H Kanamori, Y Yamada, A Nishinaka, Y Kuse, G Suzuki, T Masuda, S Nakamura, M Hosokawa, S Minatoguch, H Hara
    Sci Rep, 2020;10(1):11842.
    Species: Mouse
    Sample Types: Tissue Lysate
    Applications: Western Blot
  9. Coupling of terminal differentiation deficit with neurodegenerative pathology in Vps35-deficient pyramidal neurons
    Authors: FL Tang, L Zhao, Y Zhao, D Sun, XJ Zhu, L Mei, WC Xiong
    Cell Death Differ., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Progranulin deficiency leads to reduced glucocerebrosidase activity
    Authors: X Zhou, DH Paushter, MD Pagan, D Kim, M Nunez Sant, RL Lieberman, HS Overkleeft, Y Sun, MB Smolka, F Hu
    PLoS ONE, 2019;14(7):e0212382.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  11. Progranulin deficiency exacerbates spinal cord injury by promoting neuroinflammation and cell apoptosis in mice
    Authors: C Wang, L Zhang, JC Ndong, A Hettinghou, G Sun, C Chen, C Zhang, R Liu, CJ Liu
    J Neuroinflammation, 2019;16(1):238.
    Species: Mouse
    Sample Types: Tissue homogena
    Applications: Western Blot
  12. Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling
    Authors: F Wei, Z Jiang, H Sun, J Pu, Y Sun, M Wang, Q Tong, Y Bi, X Ma, GF Gao, J Liu
    PLoS Pathog., 2019;15(10):e1008062.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  13. Genetic Regulation of Neuronal Progranulin Reveals a Critical Role for the Autophagy-Lysosome Pathway
    Authors: LP Elia, AR Mason, A Alijagic, S Finkbeiner
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  14. Microglia increases the proliferation of retinal precursor cells during postnatal development
    Authors: Y Kuse, K Ohuchi, S Nakamura, H Hara, M Shimazawa
    Mol. Vis., 2018;24(0):536-545.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum
    Authors: N Uesaka, M Abe, K Konno, M Yamazaki, K Sakoori, T Watanabe, TH Kao, T Mikuni, M Watanabe, K Sakimura, M Kano
    Neuron, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis
    Authors: AE Arrant, VC Onyilo, DE Unger, ED Roberson
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology
    Authors: K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa
    Nat Commun, 2018;9(1):433.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity
    Authors: AM Nicholson, X Zhou, RB Perkerson, TM Parsons, J Chew, M Brooks, M DeJesus-He, NA Finch, BJ Matchett, A Kurti, KR Jansen-Wes, E Perkerson, L Daughrity, M Castanedes, L Rousseau, V Phillips, F Hu, TF Gendron, ME Murray, DW Dickson, JD Fryer, L Petrucelli, R Rademakers
    Acta Neuropathol Commun, 2018;6(1):42.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle
    Authors: H Sugihara, K Miyaji, K Yamanouchi, T Matsuwaki, M Nishihara
    J. Vet. Med. Sci., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease
    Authors: JM Van Kampen, DG Kay
    PLoS ONE, 2017;12(8):e0182896.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Progranulin functions as a cathepsin D chaperone to stimulate axonal outgrowth in vivo
    Authors: S Beel, M Moisse, M Damme, L De Muynck, W Robberecht, L Van Den Bo, P Saftig, P Van Damme
    Hum. Mol. Genet., 2017;26(15):2850-2863.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  22. Lysosomal processing of progranulin
    Authors: X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu
    Mol Neurodegener, 2017;12(1):62.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice
    Authors: TL Petkau, J Blanco, BR Leavitt
    Neurobiol. Dis., 2017;106(0):14-22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina
    Authors: Y Kuse, K Tsuruma, S Sugitani, H Izawa, Y Ohno, M Shimazawa, H Hara
    Sci Rep, 2016;6(0):23811.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells.
    Authors: Luo L, Lu L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J
    Mol Med Rep, 2014;9(5):1675-80.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Progranulin regulates neuronal outgrowth independent of sortilin.
    Authors: Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L
    Mol Neurodegener, 2012;7(0):33.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
    Biochem Biophys Res Commun, 2012;430(1):419-24.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins.
    Authors: Almeida S, Zhou L, Gao FB
    PLoS ONE, 2011;6(10):e26454.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  29. Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
    Authors: Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S
    J. Pathol., 2009;219(2):173-81.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Mouse Progranulin/PGRN Antibody

Average Rating: 5 (Based on 6 Reviews)

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Mouse Progranulin/PGRN Antibody
By Anonymous on 07/21/2021
Application: WB Sample Tested: Spinal cord Species: Mouse

Mouse Progranulin/PGRN Antibody
By Anonymous on 07/09/2021
Application: IHC Sample Tested: Cancer Tissue Species: Mouse

Mouse Progranulin/PGRN Antibody
By Anonymous on 04/30/2021
Application: IHC Sample Tested: Lung tissue Species: Mouse

Mouse Progranulin/PGRN Antibody
By Anonymous on 04/29/2021
Application: WB Sample Tested: Adult lung Species: Mouse

Mouse Progranulin/PGRN Antibody
By Anonymous on 07/21/2018
Application: WB Sample Tested: Embryonic stem cells Species: Mouse

Mouse Progranulin/PGRN Antibody
By Swati Naphade on 06/12/2016
Application: WB Sample Tested: HEK293 cell lysates transfected with mPGRN cDNA Species: Mouse

Image is from: Naphade et al., Acta Neuropathologica, 2010, 119(1):123-33