Mouse Progranulin/PGRN Antibody
Mouse Progranulin/PGRN Antibody Summary
Thr18-Leu589
Accession # P28798
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Mouse Progranulin/PGRN by Western Blot. Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85‑90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Progranulin/PGRN in Mouse Splencytes. Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Progranulin in Mouse Kidney. Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Progranulin/PGRN
Mouse Progranulin, also known as Proepithelin, PC Cell-derived Growth Factor and Acrogranin, is a secreted protein of 572 amino acid residues. It contains seven and one half granulin repeats which are characterized by 6 disulfide bridges. Progranulin can be converted in vivo to granulins by proteolysis. Progranulin is a mitogen that stimulates proliferation and survival of various epithelial, mesenchymal and tumor cells. Several granulins have also been shown to be biologically active and may play a role in inflammation and wound healing. Mouse Progranulin shares 86% and 75% amino acid sequence homology with rat and human Progranulin, respectively.
Product Datasheets
Citations for Mouse Progranulin/PGRN Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
18
Citations: Showing 1 - 10
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Progranulin deficiency leads to reduced glucocerebrosidase activity
Authors: X Zhou, DH Paushter, MD Pagan, D Kim, M Nunez Sant, RL Lieberman, HS Overkleeft, Y Sun, MB Smolka, F Hu
PLoS ONE, 2019;14(7):e0212382.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Genetic Regulation of Neuronal Progranulin Reveals a Critical Role for the Autophagy-Lysosome Pathway
Authors: LP Elia, AR Mason, A Alijagic, S Finkbeiner
J. Neurosci., 2019;0(0):.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Detection) -
Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity
Authors: AM Nicholson, X Zhou, RB Perkerson, TM Parsons, J Chew, M Brooks, M DeJesus-He, NA Finch, BJ Matchett, A Kurti, KR Jansen-Wes, E Perkerson, L Daughrity, M Castanedes, L Rousseau, V Phillips, F Hu, TF Gendron, ME Murray, DW Dickson, JD Fryer, L Petrucelli, R Rademakers
Acta Neuropathol Commun, 2018;6(1):42.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum
Authors: N Uesaka, M Abe, K Konno, M Yamazaki, K Sakoori, T Watanabe, TH Kao, T Mikuni, M Watanabe, K Sakimura, M Kano
Neuron, 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Microglia increases the proliferation of retinal precursor cells during postnatal development
Authors: Y Kuse, K Ohuchi, S Nakamura, H Hara, M Shimazawa
Mol. Vis., 2018;24(0):536-545.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology
Authors: K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa
Nat Commun, 2018;9(1):433.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis
Authors: AE Arrant, VC Onyilo, DE Unger, ED Roberson
J. Neurosci., 2018;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease
Authors: JM Van Kampen, DG Kay
PLoS ONE, 2017;12(8):e0182896.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Progranulin functions as a cathepsin D chaperone to stimulate axonal outgrowth in vivo
Authors: S Beel, M Moisse, M Damme, L De Muynck, W Robberecht, L Van Den Bo, P Saftig, P Van Damme
Hum. Mol. Genet., 2017;26(15):2850-2863.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle
Authors: H Sugihara, K Miyaji, K Yamanouchi, T Matsuwaki, M Nishihara
J. Vet. Med. Sci., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lysosomal processing of progranulin
Authors: X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu
Mol Neurodegener, 2017;12(1):62.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice
Authors: TL Petkau, J Blanco, BR Leavitt
Neurobiol. Dis., 2017;106(0):14-22.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina
Authors: Y Kuse, K Tsuruma, S Sugitani, H Izawa, Y Ohno, M Shimazawa, H Hara
Sci Rep, 2016;6(0):23811.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells.
Authors: Luo L, Lu L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J
Mol Med Rep, 2014;9(5):1675-80.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Progranulin regulates neuronal outgrowth independent of sortilin.
Authors: Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L
Mol Neurodegener, 2012;7(0):33.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
Biochem Biophys Res Commun, 2012;430(1):419-24.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins.
Authors: Almeida S, Zhou L, Gao FB
PLoS ONE, 2011;6(10):e26454.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
Authors: Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S
J. Pathol., 2009;219(2):173-81.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
FAQs
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Reviews for Mouse Progranulin/PGRN Antibody
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Image is from: Naphade et al., Acta Neuropathologica, 2010, 119(1):123-33
