Mouse Progranulin/PGRN Antibody Summary
Thr18-Leu589
Accession # P28798
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Mouse Progranulin/PGRN by Western Blot. Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Progranulin in Mouse Kidney. Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Progranulin/PGRN in Mouse Splencytes. Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Progranulin/PGRN
Mouse Progranulin, also known as Proepithelin, PC Cell-derived Growth Factor and Acrogranin, is a secreted protein of 572 amino acid residues. It contains seven and one half granulin repeats which are characterized by 6 disulfide bridges. Progranulin can be converted in vivo to granulins by proteolysis. Progranulin is a mitogen that stimulates proliferation and survival of various epithelial, mesenchymal and tumor cells. Several granulins have also been shown to be biologically active and may play a role in inflammation and wound healing. Mouse Progranulin shares 86% and 75% amino acid sequence homology with rat and human Progranulin, respectively.
Product Datasheets
Citations for Mouse Progranulin/PGRN Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Decreased Prosaposin and Progranulin in the Cingulate Cortex Are Associated with Schizophrenia Pathophysiology
Authors: Y He, X Zhang, I Flais, P Svenningss
International Journal of Molecular Sciences, 2022;23(19):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Progranulin Preserves Autophagy Flux and Mitochondrial Function in Rat Cortical Neurons Under High Glucose Stress
Authors: C Dedert, V Mishra, G Aggarwal, AD Nguyen, F Xu
Frontiers in Cellular Neuroscience, 2022;16(0):874258.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis
Authors: MN Santos, DH Paushter, T Zhang, X Wu, T Feng, J Lou, H Du, SM Becker, R Fragoza, H Yu, F Hu
The Journal of Biological Chemistry, 2022;0(0):102348.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Differential regulation of progranulin derived granulin peptides
Authors: T Zhang, H Du, MN Santos, X Wu, MD Pagan, LJ Trigiani, N Nishimura, T Reinheckel, F Hu
Molecular Neurodegeneration, 2022;17(1):15.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice
Authors: M Murakoshi, T Gohda, E Adachi, S Ichikawa, S Hagiwara, Y Suzuki
Scientific Reports, 2021;11(1):1194.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation
Authors: K Takahashi, S Nakamura, W Otsu, M Shimazawa, H Hara
Journal of Neuroinflammation, 2021;18(1):164.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
Authors: J Frew, HB Nygaard
Acta neuropathologica communications, 2021;9(1):57.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Effects of progranulin on the pathological conditions in experimental myocardial infarction model
Authors: T Sasaki, M Shimazawa, H Kanamori, Y Yamada, A Nishinaka, Y Kuse, G Suzuki, T Masuda, S Nakamura, M Hosokawa, S Minatoguch, H Hara
Sci Rep, 2020;10(1):11842.
Species: Mouse
Sample Types: Tissue Lysate
Applications: Western Blot -
Coupling of terminal differentiation deficit with neurodegenerative pathology in Vps35-deficient pyramidal neurons
Authors: FL Tang, L Zhao, Y Zhao, D Sun, XJ Zhu, L Mei, WC Xiong
Cell Death Differ., 2020;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Progranulin deficiency leads to reduced glucocerebrosidase activity
Authors: X Zhou, DH Paushter, MD Pagan, D Kim, M Nunez Sant, RL Lieberman, HS Overkleeft, Y Sun, MB Smolka, F Hu
PLoS ONE, 2019;14(7):e0212382.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Progranulin deficiency exacerbates spinal cord injury by promoting neuroinflammation and cell apoptosis in mice
Authors: C Wang, L Zhang, JC Ndong, A Hettinghou, G Sun, C Chen, C Zhang, R Liu, CJ Liu
J Neuroinflammation, 2019;16(1):238.
Species: Mouse
Sample Types: Tissue homogena
Applications: Western Blot -
Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling
Authors: F Wei, Z Jiang, H Sun, J Pu, Y Sun, M Wang, Q Tong, Y Bi, X Ma, GF Gao, J Liu
PLoS Pathog., 2019;15(10):e1008062.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Genetic Regulation of Neuronal Progranulin Reveals a Critical Role for the Autophagy-Lysosome Pathway
Authors: LP Elia, AR Mason, A Alijagic, S Finkbeiner
J. Neurosci., 2019;0(0):.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Detection) -
Microglia increases the proliferation of retinal precursor cells during postnatal development
Authors: Y Kuse, K Ohuchi, S Nakamura, H Hara, M Shimazawa
Mol. Vis., 2018;24(0):536-545.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum
Authors: N Uesaka, M Abe, K Konno, M Yamazaki, K Sakoori, T Watanabe, TH Kao, T Mikuni, M Watanabe, K Sakimura, M Kano
Neuron, 2018;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis
Authors: AE Arrant, VC Onyilo, DE Unger, ED Roberson
J. Neurosci., 2018;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology
Authors: K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa
Nat Commun, 2018;9(1):433.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity
Authors: AM Nicholson, X Zhou, RB Perkerson, TM Parsons, J Chew, M Brooks, M DeJesus-He, NA Finch, BJ Matchett, A Kurti, KR Jansen-Wes, E Perkerson, L Daughrity, M Castanedes, L Rousseau, V Phillips, F Hu, TF Gendron, ME Murray, DW Dickson, JD Fryer, L Petrucelli, R Rademakers
Acta Neuropathol Commun, 2018;6(1):42.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle
Authors: H Sugihara, K Miyaji, K Yamanouchi, T Matsuwaki, M Nishihara
J. Vet. Med. Sci., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease
Authors: JM Van Kampen, DG Kay
PLoS ONE, 2017;12(8):e0182896.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Progranulin functions as a cathepsin D chaperone to stimulate axonal outgrowth in vivo
Authors: S Beel, M Moisse, M Damme, L De Muynck, W Robberecht, L Van Den Bo, P Saftig, P Van Damme
Hum. Mol. Genet., 2017;26(15):2850-2863.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Lysosomal processing of progranulin
Authors: X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu
Mol Neurodegener, 2017;12(1):62.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice
Authors: TL Petkau, J Blanco, BR Leavitt
Neurobiol. Dis., 2017;106(0):14-22.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina
Authors: Y Kuse, K Tsuruma, S Sugitani, H Izawa, Y Ohno, M Shimazawa, H Hara
Sci Rep, 2016;6(0):23811.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells.
Authors: Luo L, Lu L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J
Mol Med Rep, 2014;9(5):1675-80.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Progranulin regulates neuronal outgrowth independent of sortilin.
Authors: Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L
Mol Neurodegener, 2012;7(0):33.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
Biochem Biophys Res Commun, 2012;430(1):419-24.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins.
Authors: Almeida S, Zhou L, Gao FB
PLoS ONE, 2011;6(10):e26454.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
Authors: Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S
J. Pathol., 2009;219(2):173-81.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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Reviews for Mouse Progranulin/PGRN Antibody
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