Mouse Progranulin/PGRN Antibody
Mouse Progranulin/PGRN Antibody Summary
Thr18-Leu589
Accession # P28798
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
View Larger
Detection of Mouse Progranulin/PGRN by Western Blot. Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85‑90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Progranulin/PGRN in Mouse Splencytes. Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
View Larger
Progranulin in Mouse Kidney. Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Progranulin/PGRN
Mouse Progranulin, also known as Proepithelin, PC Cell-derived Growth Factor and Acrogranin, is a secreted protein of 572 amino acid residues. It contains seven and one half granulin repeats which are characterized by 6 disulfide bridges. Progranulin can be converted in vivo to granulins by proteolysis. Progranulin is a mitogen that stimulates proliferation and survival of various epithelial, mesenchymal and tumor cells. Several granulins have also been shown to be biologically active and may play a role in inflammation and wound healing. Mouse Progranulin shares 86% and 75% amino acid sequence homology with rat and human Progranulin, respectively.
Product Datasheets
Citations for Mouse Progranulin/PGRN Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Genetic Regulation of Neuronal Progranulin Reveals a Critical Role for the Autophagy-Lysosome Pathway
Authors: LP Elia, AR Mason, A Alijagic, S Finkbeiner
J. Neurosci., 2019;0(0):.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Detection) -
Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology
Authors: K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa
Nat Commun, 2018;9(1):433.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity
Authors: AM Nicholson, X Zhou, RB Perkerson, TM Parsons, J Chew, M Brooks, M DeJesus-He, NA Finch, BJ Matchett, A Kurti, KR Jansen-Wes, E Perkerson, L Daughrity, M Castanedes, L Rousseau, V Phillips, F Hu, TF Gendron, ME Murray, DW Dickson, JD Fryer, L Petrucelli, R Rademakers
Acta Neuropathol Commun, 2018;6(1):42.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis
Authors: AE Arrant, VC Onyilo, DE Unger, ED Roberson
J. Neurosci., 2018;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Microglia increases the proliferation of retinal precursor cells during postnatal development
Authors: Y Kuse, K Ohuchi, S Nakamura, H Hara, M Shimazawa
Mol. Vis., 2018;24(0):536-545.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle
Authors: H Sugihara, K Miyaji, K Yamanouchi, T Matsuwaki, M Nishihara
J. Vet. Med. Sci., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice
Authors: TL Petkau, J Blanco, BR Leavitt
Neurobiol. Dis., 2017;106(0):14-22.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lysosomal processing of progranulin
Authors: X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu
Mol Neurodegener, 2017;12(1):62.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease
Authors: JM Van Kampen, DG Kay
PLoS ONE, 2017;12(8):e0182896.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina
Authors: Y Kuse, K Tsuruma, S Sugitani, H Izawa, Y Ohno, M Shimazawa, H Hara
Sci Rep, 2016;6(0):23811.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells.
Authors: Luo L, Lu L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J
Mol Med Rep, 2014;9(5):1675-80.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
Biochem Biophys Res Commun, 2012;430(1):419-24.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Progranulin regulates neuronal outgrowth independent of sortilin.
Authors: Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L
Mol Neurodegener, 2012;7(0):33.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins.
Authors: Almeida S, Zhou L, Gao FB
PLoS ONE, 2011;6(10):e26454.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
Authors: Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S
J. Pathol., 2009;219(2):173-81.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsCell and Tissue Staining Kits
Immunohistochemistry Reagents
Isotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Mouse Progranulin/PGRN Antibody
Average Rating: 5 (Based on 2 Reviews)
Have you used Mouse Progranulin/PGRN Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Image is from: Naphade et al., Acta Neuropathologica, 2010, 119(1):123-33
