Mouse/Rat IGF-I Quantikine ELISA Kit

(41 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Tissue Homogenates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    8.4 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse and rat IGF-I
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat IGF-I Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IGF-I in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse IGF-I and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse and rat IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat IGF-I.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation4.68.937.77.811.837.4


The recovery of mouse/rat IGF-I spiked to three levels throughout the range of the assay in the cell culture supernate samples was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=7) 98 89-111
Rat Cell Culture Supernates (n=6) 98 82-108
To assess the linearity of the assay, samples containing mouse or rat IGF-I in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse/Rat IGF-I Quantikine ELISA Kit
Mouse/Rat IGF-I Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

    • Long Name
      Insulin-like Growth Factor I
    • Entrez Gene IDs
      3479 (Human); 16000 (Mouse); 24482 (Rat);
    • Alternate Names
      IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 41
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    Sample Type
    1. Single ingestion of soy ?-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects
      Authors: Tsutomu Hashidume
      Sci Rep, 2016;6(0):28183.
      Species: Mouse
      Sample Type: Serum
    2. Naturally occurring p16(Ink4a)-positive cells shorten healthy lifespan.
      Authors: Baker D, Childs B, Durik M, Wijers M, Sieben C, Zhong J, Saltness R, Jeganathan K, Verzosa G, Pezeshki A, Khazaie K, Miller J, van Deursen J
      Nature, 2015;530(7589):184-9.
      Species: Mouse
      Sample Type: Serum
    3. Microglial Kv1.3 Channels and P2Y12 Receptors Differentially Regulate Cytokine and Chemokine Release from Brain Slices of Young Adult and Aged Mice.
      Authors: Charolidi N, Schilling T, Eder C
      PLoS ONE, 2015;10(5):e0128463.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    4. MeCP2 Affects Skeletal Muscle Growth and Morphology through Non Cell-Autonomous Mechanisms.
      Authors: Conti V, Gandaglia A, Galli F, Tirone M, Bellini E, Campana L, Kilstrup-Nielsen C, Rovere-Querini P, Brunelli S, Landsberger N
      PLoS ONE, 2015;10(6):e0130183.
      Species: Mouse
      Sample Type: Plasma
    5. Skeletal muscle mitochondrial uncoupling drives endocrine cross-talk through the induction of FGF21 as a myokine.
      Authors: Keipert S, Ost M, Johann K, Imber F, Jastroch M, van Schothorst E, Keijer J, Klaus S
      Am J Physiol Endocrinol Metab, 2014;306(5):E469-82.
      Species: Mouse
      Sample Type: Plasma
    6. Long-term physiological alterations and recovery in a mouse model of separation associated with time-restricted feeding: a tool to study anorexia nervosa related consequences.
      Authors: Zgheib S, Mequinion M, Lucas S, Leterme D, Ghali O, Tolle V, Zizzari P, Bellefontaine N, Legroux-Gerot I, Hardouin P, Broux O, Viltart O, Chauveau C
      PLoS ONE, 2014;9(8):e103775.
      Species: Mouse
      Sample Type: Plasma
    7. Sonic hedgehog gene therapy increases the ability of the dystrophic skeletal muscle to regenerate after injury.
      Authors: Piccioni A, Gaetani E, Palladino M, Gatto I, Smith R, Neri V, Marcantoni M, Giarretta I, Silver M, Straino S, Capogrossi M, Landolfi R, Pola R
      Gene Ther, 2014;21(4):413-21.
      Species: Mouse
      Sample Type: cell supernates
    8. Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling.
      Authors: Hwang D, Shin H, Kwon M, Choi J, Ryu B, Kim B
      J Neurosci, 2014;34(38):12788-800.
      Species: Rat
      Sample Type: Serum
    9. The small-molecule BGP-15 protects against heart failure and atrial fibrillation in mice.
      Authors: Sapra, Geeta, Tham, Yow Keat, Cemerlang, Nelly, Matsumoto, Aya, Kiriazis, Helen, Bernardo, Bianca C, Henstridge, Darren C, Ooi, Jenny Y, Pretorius, Lynette, Boey, Esther J, Lim, Lydia, Sadoshima, Junichi, Meikle, Peter J, Mellet, Natalie, Woodcock, Elizabet, Marasco, Silvana, Ueyama, Tomomi, Du, Xiao-Jun, Febbraio, Mark A, McMullen, Julie R
      Nat Commun, 2014;5(0):5705.
      Species: Mouse
      Sample Type: Plasma
    10. Calorie restriction decreases murine and human pancreatic tumor cell growth, nuclear factor-kappaB activation, and inflammation-related gene expression in an insulin-like growth factor-1-dependent manner.
      Authors: Harvey A, Lashinger L, Hays D, Harrison L, Lewis K, Fischer S, Hursting S
      PLoS ONE, 2014;9(5):e94151.
      Species: Mouse
      Sample Type: Serum
    11. The dwarf phenotype in GH240B mice, haploinsufficient for the autism candidate gene Neurobeachin, is caused by ectopic expression of recombinant human growth hormone.
      Authors: Nuytens, Kim, Tuand, Krizia, Fu, Quili, Stijnen, Pieter, Pruniau, Vincent, Meulemans, Sandra, Vankelecom, Hugo, Creemers, John W M
      PLoS ONE, 2014;9(10):e109598.
      Species: Mouse
      Sample Type: Plasma
    12. Bone resorption is regulated by cell-autonomous negative feedback loop of Stat5-Dusp axis in the osteoclast.
      Authors: Hirose J, Masuda H, Tokuyama N, Omata Y, Matsumoto T, Yasui T, Kadono Y, Hennighausen L, Tanaka S
      J Exp Med, 2014;211(1):153-63.
      Species: Mouse
      Sample Type: Serum
    13. The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function.
      Authors: Takehara, Yuji, Yabuuchi, Akiko, Ezoe, Kenji, Kuroda, Tomoko, Yamadera, Rie, Sano, Chiaki, Murata, Nana, Aida, Takuya, Nakama, Ken, Aono, Fumihito, Aoyama, Naoki, Kato, Keiich, Kato, Osamu
      Lab Invest, 2013;93(2):181-93.
      Species: Rat
      Sample Type: Cell Culture Supernates
    14. SIRT1 regulates adaptive response of the growth hormone--insulin-like growth factor-I axis under fasting conditions in liver.
      Authors: Yamamoto, Masaaki, Iguchi, Genzo, Fukuoka, Hidenori, Suda, Kentaro, Bando, Hironori, Takahashi, Michiko, Nishizawa, Hitoshi, Seino, Susumu, Takahashi, Yutaka
      Proc Natl Acad Sci U S A, 2013;110(37):14948-53.
      Species: Mouse
      Sample Type: Serum
    15. Splicing factor SRSF3 is crucial for hepatocyte differentiation and metabolic function.
      Authors: Sen S, Jumaa H, Webster N
      Nat Commun, 2013;4(0):1336.
      Species: Mouse
      Sample Type: Serum
    16. Growth hormone secretion is correlated with neuromuscular innervation rather than motor neuron number in early-symptomatic male amyotrophic lateral sclerosis mice.
      Authors: Steyn, F J, Lee, K, Fogarty, M J, Veldhuis, J D, McCombe, P A, Bellingham, M C, Ngo, S T, Chen, C
      Endocrinology, 2013;154(12):4695-706.
      Species: Mouse
      Sample Type: Plasma
    17. IGF1 receptor signaling regulates adaptive radioprotection in glioma stem cells.
      Authors: Osuka S, Sampetrean O, Shimizu T, Saga I, Onishi N, Sugihara E, Okubo J, Fujita S, Takano S, Matsumura A, Saya H
      Stem Cells, 2013;31(4):627-40.
      Species: Mouse
      Sample Type: Cell Lysate
    18. Fish oil slows prostate cancer xenograft growth relative to other dietary fats and is associated with decreased mitochondrial and insulin pathway gene expression.
      Authors: Lloyd J, Masko E, Wu C, Keenan M, Pilla D, Aronson W, Chi J, Freedland S
      Prostate Cancer Prostatic Dis, 2013;16(4):285-91.
      Species: Mouse
      Sample Type: Serum
    19. BMP9 ameliorates amyloidosis and the cholinergic defect in a mouse model of Alzheimer's disease.
      Authors: Burke R, Norman T, Haydar T, Slack B, Leeman S, Blusztajn J, Mellott T
      Proc Natl Acad Sci U S A, 2013;110(48):19567-72.
      Species: Mouse
      Sample Type: Tissue Homogenates
    20. Effects of breastfeeding on body composition and maturational tempo in the rat.
      Authors: Crispel, Yonatan, Katz, Oren, Ben-Yosef, Dafna, Hochberg, Ze'ev
      BMC Med, 2013;11(0):114.
      Species: Rat
      Sample Type: Serum
    21. Cytosolic Malic Enzyme 1 (ME1) Mediates High Fat Diet-Induced Adiposity, Endocrine Profile, and Gastrointestinal Tract Proliferation-Associated Biomarkers in Male Mice.
      Authors: Al-Dwairi, Ahmed, Pabona, John Mar, Simmen, Rosalia, Simmen, Frank A
      PLoS ONE, 2012;7(10):e46716.
      Species: Mouse
      Sample Type: Serum
    22. Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway.
      Authors: Ma B, Zhang Q, Wu D, Wang YL, Hu YY, Cheng YP, Yang ZD, Zheng YY, Ying HJ
      Acta Pharmacol. Sin., 2012;33(4):479-89.
      Species: Rat
      Sample Type: Serum
    23. Differential utilization of ketone bodies by neurons and glioma cell lines: a rationale for ketogenic diet as experimental glioma therapy.
      Authors: Maurer GD, Brucker DP, Bahr O, Harter PN, Hattingen E, Walenta S, Mueller-Klieser W, Steinbach JP, Rieger J
      BMC Cancer, 2011;11(0):315.
      Species: Mouse
      Sample Type: Serum
    24. Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function.
      Authors: Sato H, Isogai Y, Masuda S
      J. Biol. Chem., 2011;286(13):11632-48.
      Species: Mouse
      Sample Type: Serum
    25. Human IGF Binding Protein-3 Overexpression Impairs Glucose Regulation in Mice via an Inhibition of Insulin Secretion.
      Authors: Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL
      Endocrinology, 2011;152(6):2184-96.
      Species: Mouse
      Sample Type: Plasma
    26. Peripheral SMN restoration is essential for long-term rescue of a severe spinal muscular atrophy mouse model.
      Authors: Hua Y, Sahashi K, Rigo F
      Nature, 2011;478(7367):123-6.
      Species: Mouse
      Sample Type: Serum
    27. Rim2alpha determines docking and priming States in insulin granule exocytosis.
      Authors: Yasuda T, Shibasaki T, Minami K
      Cell Metab., 2010;12(2):117-29.
      Species: Mouse
      Sample Type: Serum
    28. Galectin-3 contributes to neonatal hypoxic-ischemic brain injury.
      Authors: Doverhag C, Hedtjarn M, Poirier F, Mallard C, Hagberg H, Karlsson A, Savman K
      Neurobiol. Dis., 2010;38(1):36-46.
      Species: Mouse
      Sample Type: Tissue Homogenates
    29. Myocytic androgen receptor controls the strength but not the mass of limb muscles.
      Authors: Chambon C, Duteil D, Vignaud A, Ferry A, Messaddeq N, Malivindi R, Kato S, Chambon P, Metzger D
      Proc. Natl. Acad. Sci. U.S.A., 2010;107(32):14327-32.
      Species: Mouse
      Sample Type: Plasma
    30. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
      Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
      J. Neurochem., 2009;111(6):1409-24.
      Species: Mouse
      Sample Type: Tissue Homogenates
    31. Modulation of melanocortin signaling ameliorates uremic cachexia.
      Authors: Cheung WW, Rosengren S, Boyle DL, Mak RH
      Kidney Int., 2008;74(2):180-6.
      Species: Mouse
      Sample Type: Tissue Homogenates
    32. Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat.
      Authors: Kubota Y, Unoki H, Bujo H, Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M, Saito Y
      Biochem. Biophys. Res. Commun., 2008;368(1):81-7.
      Species: Mouse
      Sample Type: Plasma
    33. Insulin-like growth factor-1 improves survival in sepsis via enhanced hepatic bacterial clearance.
      Authors: Ashare A, Nymon AB, Doerschug KC, Morrison JM, Monick MM, Hunninghake GW
      Am. J. Respir. Crit. Care Med., 2008;178(2):149-57.
      Species: Mouse
      Sample Type: Serum
    34. Reduction in hypophyseal growth hormone and prolactin expression due to deficiency in ghrelin receptor signaling is associated with Pit-1 suppression: Relevance to the immune system.
      Authors: Yang H, Dixit VD, Patel K, Vandanmagsar B, Collins G, Sun Y, Smith RG, Taub DD
      Brain Behav. Immun., 2008;0(0):.
      Species: Mouse
      Sample Type: Serum
    35. Inhibition of rho family functions by lovastatin promotes myelin repair in ameliorating experimental autoimmune encephalomyelitis.
      Authors: Paintlia AS, Paintlia MK, Singh AK, Singh I
      Mol. Pharmacol., 2008;73(5):1381-93.
      Species: Rat
      Sample Type: Serum
    36. GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion.
      Authors: Wanderling S, Simen BB, Ostrovsky O, Ahmed NT, Vogen SM, Gidalevitz T, Argon Y
      Mol. Biol. Cell, 2007;18(10):3764-75.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    37. Ovariectomy is protective against renal injury in the high-salt-fed older mRen2.Lewis rat.
      Authors: Yamaleyeva LM, Pendergrass KD, Pirro NT, Gallagher PE, Groban L, Chappell MC
      Am. J. Physiol. Heart Circ. Physiol., 2007;293(4):H2064-71.
      Species: Rat
      Sample Type: Plasma
    38. Wheel running and fluoxetine antidepressant treatment have differential effects in the hippocampus and the spinal cord.
      Authors: Engesser-Cesar C, Anderson AJ, Cotman CW
      Neuroscience, 2007;144(3):1033-44.
      Species: Mouse
      Sample Type: Serum
    39. Microcytosis in ank/ank mice and the role of ANKH in promoting erythroid differentiation.
      Authors: Wang J, Wang C, Tsui HW, Las Heras F, Cheng EY, Iscove NN, Chiu B, Inman RD, Pritzker KP, Tsui FW
      Exp. Cell Res., 2007;313(20):4120-9.
      Species: Mouse
      Sample Type: Serum
    40. Ghrelin promotes thymopoiesis during aging.
      Authors: Dixit VD, Yang H, Sun Y, Weeraratna AT, Youm YH, Smith RG, Taub DD
      J. Clin. Invest., 2007;117(10):2778-90.
      Species: Mouse
      Sample Type: Serum
    41. Peripheral elevation of IGF-1 fails to alter Abeta clearance in multiple in vivo models.
      Authors: Lanz TA, Salatto CT, Semproni AR, Marconi M, Brown TM, Richter KE, Schmidt K, Nelson FR, Schachter JB
      Biochem. Pharmacol., 2007;75(5):1093-103.
      Species: Rat
      Sample Type: Plasma
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