Mouse/Rat IL-10 Antibody
Mouse/Rat IL-10 Antibody Summary
Ser19-Asn178
Accession # P29456
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Recombinant Mouse and Rat IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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Cell Proliferation Induced by IL‑10 and Neutralization by Rat IL‑10 Antibody. Recombinant Rat IL-10 (Catalog # 522-RL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519). The ND50 is typically 0.3-1.0 µg/mL.
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IL‑10 in Mouse Splenocytes. IL‑10 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse/Rat IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10
Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL‑22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature rat IL‑10 shares 85% amino acid sequence identity with mouse and 71%‑79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8‑10). The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12‑14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15‑17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
- O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
- Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
- Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
- Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
- Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
- Hsu, D.-H. et al. (1990) Science 250:830.
- Goodman, R. et al. (1992) Biochem. Biophys. Res. Commun. 189:1.
- Windsor, W.T. et al. (1993) Biochemistry 32:8807.
- Syto, R. et al. (1998) Biochemistry 37:16943.
- Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
- Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
- Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
- Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
- Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
- Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
- Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.
Product Datasheets
Citations for Mouse/Rat IL-10 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exercise
Authors: T Komori, Y Morikawa
The Journal of Biological Chemistry, 2022-11-09;0(0):102686.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cellular Sources and Neuroprotective Roles of Interleukin-10 in the Facial Motor Nucleus after Axotomy
Authors: EM Runge, DO Setter, AK Iyer, EJ Regele, FM Kennedy, VM Sanders, KJ Jones
Cells, 2022-10-09;11(19):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Inhibition of Serine Protease Activity Protects Against High Fat Diet-Induced Inflammation and Insulin Resistance
Authors: CS Kuo, JS Chen, LY Lin, GW Schmid-Sch, S Chien, PH Huang, JW Chen, SJ Lin
Sci Rep, 2020-02-03;10(1):1725.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Involvement of Interleukin-10 in Analgesia of Electroacupuncture on Incision Pain
Authors: WJ Dai, JL Sun, C Li, W Mao, YK Huang, ZQ Zhao, YQ Zhang, N Lü
Evid Based Complement Alternat Med, 2019-10-30;2019(0):8413576.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury
Authors: S Zilberman-, R Abu-Hamad, R Zarura, M Sova, Y Hachmo, M Stark, S Neuman, S Slavin, S Efrati
PLoS ONE, 2019-09-12;14(9):e0222354.
Species: Rat
Sample Types: Whole Tissue
Applications: Fluorescence Microscopy -
Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/?-endorphin pathway
Authors: XF Mao, HY Wu, XQ Tang, U Ali, H Liu, YX Wang
J Neuroinflammation, 2019-04-13;16(1):84.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Blocking p38 Signaling Reduces the Activation of Pro-inflammatory Cytokines and the Phosphorylation of p38 in the Habenula and Reverses Depressive-Like Behaviors Induced by Neuroinflammation
Authors: YW Zhao, YQ Pan, MM Tang, WJ Lin
Front Pharmacol, 2018-05-15;9(0):511.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Autocrine interleukin-10 mediates glucagon-like peptide-1 receptor-induced spinal microglial ?-endorphin expression
Authors: HY Wu, XQ Tang, XF Mao, YX Wang
J. Neurosci., 2017-10-30;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN?
Authors: JE Lim, E Chung, Y Son
Sci Rep, 2017-08-25;7(1):9417.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
J. Neurosci., 2017-04-07;0(0):.
Species: Mouse, Rat
Sample Types: Whole Cells
Applications: Neutralization -
Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization
Authors: M Viladomiu, J Bassaganya, N Tubau-Juni, B Kronsteine, A Leber, CW Philipson, V Zoccoli-Ro, R Hontecilla
J. Immunol, 2017-03-06;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion
Authors: L Cambier, G de Couto, A Ibrahim, AK Echavez, J Valle, W Liu, M Kreke, RR Smith, L Marbán, E Marbán
EMBO Mol Med, 2017-03-01;9(3):337-352.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
Immunization with a Myelin-Derived Antigen Activates the Brain's Choroid Plexus for Recruitment of Immunoregulatory Cells to the CNS and Attenuates Disease Progression in a Mouse Model of ALS.
Authors: Kunis G, Baruch K, Miller O, Schwartz M
J Neurosci, 2015-04-22;35(16):6381-93.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10.
Authors: Xie L, Choudhury G, Winters A, Yang S, Jin K
Eur J Immunol, 2014-11-24;45(1):180-91.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
CNS repair requires both effector and regulatory T cells with distinct temporal and spatial profiles.
Authors: Raposo C, Graubardt N, Cohen M, Eitan C, London A, Berkutzki T, Schwartz M
J Neurosci, 2014-07-30;34(31):10141-55.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
Authors: Furuhashi, Kazuhiro, Tsuboi, Naotake, Shimizu, Asuka, Katsuno, Takayuki, Kim, Hangsoo, Saka, Yosuke, Ozaki, Takenori, Sado, Yoshikaz, Imai, Enyu, Matsuo, Seiichi, Maruyama, Shoichi
J Am Soc Nephrol, 2013-03-07;24(4):587-603.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Functionalized scaffold-mediated interleukin 10 gene delivery significantly improves survival rates of stem cells in vivo.
Authors: Holladay C, Power K, Sefton M, O'Brien T, Gallagher WM, Pandit A
Mol. Ther., 2011-01-25;19(5):969-78.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
Authors: Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
Invest. Ophthalmol. Vis. Sci., 2008-05-16;49(9):3999-4007.
Species: Rat
Sample Types: Aqueous Humor
Applications: Neutralization
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Reviews for Mouse/Rat IL-10 Antibody
Average Rating: 4.6 (Based on 5 Reviews)
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Try this for flow, the signal is not too strong, may need optimize.
We had an issue with shipping due to COVID, that caused the antibody to sit at room temperature for too long and become unusable. Fortunately we placed another order of the exact same product number, and it performed perfectly. I've included the photo of the gel after the shipping mishap
This image was purely a test with an IL-10 control protein at different concentrations. Initially we were unable to detect the IL-10 protein in our samples, so we were testing to ensure that this antibody was functional. Turns out it was!
