Mouse/Rat IL-5 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF545
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Mouse/Rat IL-5 Biotinylated Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects rat and mouse IL-5 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human IL-5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat IL-5
Met20-Val132
Accession # Q08125
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat IL‑5 (Catalog # 545-RL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs formurine, human and rat IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113, 115, and 113 amino acid residues, respectively. Rat IL-5 is 94% and 70% identical to mouse and human IL-5, respectively. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked tothe genes for IL-3, IL-4 and GM-CSF.

IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high-affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is sharedwith the high-affinity receptors for GM-CSF and IL-3.

References
  1. Karlen, S. et al. (1998) Intern. Rev. Immunol. 16:227.
  2. Lalani, T. et al. (1999) Annals Allergy Asthma Immunol. 82:317.
  3. Takatsa, K. (1998) Cytokine and Growth Factor Reviews 9:25.
Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

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