Detects rat IL‑5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL‑5 is observed and 5% cross‑reactivity with recombinant human IL‑5 is observed.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat IL-5 Met20-Val132 Accession # Q08125
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 1.25 ng/mL Recombinant Rat IL‑5.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑5 and Neutralization by Rat IL‑5 Antibody.
Recombinant Rat IL‑5 (Catalog # 545-RL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF545). The ND50 is typically 2‑6 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs formurine, human and rat IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113, 115, and 113 amino acid residues, respectively. Rat IL-5 is 94% and 70% identical to mouse and human IL-5, respectively. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked tothe genes for IL-3, IL-4 and GM-CSF.
IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high-affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is sharedwith the high-affinity receptors for GM-CSF and IL-3.
Karlen, S. et al. (1998) Intern. Rev. Immunol. 16:227.
Lalani, T. et al. (1999) Annals Allergy Asthma Immunol. 82:317.
Takatsa, K. (1998) Cytokine and Growth Factor Reviews 9:25.
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