Rat IL-5 Antibody

    
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  • Species Reactivity
    Rat
  • Specificity
    Detects rat IL‑5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL‑5 is observed and 5% cross‑reactivity with recombinant human IL‑5 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat IL-5
    Met20-Val132
    Accession # Q08125
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat IL‑5 (Catalog # 545-RL)
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed rat splenocytes
  • Neutralization
    Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 1.25 ng/mL Recombinant Rat IL‑5.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Cell Proliferation Induced by IL‑5 and Neutralization by Rat IL‑5 Antibody.

Recombinant Rat IL‑5 (Catalog # 545-RL) stimulates proliferation in the TF‑1 human erythro­leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF545). The ND50 is typically 2‑6 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5

Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs formurine, human and rat IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113, 115, and 113 amino acid residues, respectively. Rat IL-5 is 94% and 70% identical to mouse and human IL-5, respectively. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked tothe genes for IL-3, IL-4 and GM-CSF.

IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high-affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is sharedwith the high-affinity receptors for GM-CSF and IL-3.

  • References:
    1. Karlen, S. et al. (1998) Intern. Rev. Immunol. 16:227.
    2. Lalani, T. et al. (1999) Annals Allergy Asthma Immunol. 82:317.
    3. Takatsa, K. (1998) Cytokine and Growth Factor Reviews 9:25.
  • Long Name:
    Interleukin 5
  • Entrez Gene IDs:
    3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
  • Alternate Names:
    BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
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