Mouse/Rat Leptin Quantikine ELISA Kit

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Mouse/Rat Leptin/OB ELISA Standard Curve
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Product Details
Procedure
Citations (110)
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Mouse/Rat Leptin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL)
Sensitivity
22 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse and rat Leptin.  This assay cross-reacts 0.24% with Human Leptin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse Leptin Immunoassay is a 4.5 hour solid phase ELISA designed to measure soluble mouse leptin in cell culture supernates, serum*, and plasma. It contains recombinant mouse leptin expressed in E. coli and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse leptin accurately. Results obtained measuring natural mouse leptin showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse leptin.

*Pregnant mouse serum from the second trimester of pregnancy through post-partum day 1 require acid/urea activation of mouse leptin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 182 693 1803 187 654 1736
Standard Deviation 7.9 22.6 67.9 14.2 30.5 87.4
CV% 4.3 3.3 3.8 7.6 4.7 5

Recovery

The recovery of mouse/rat Leptin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=5) 101 85-120
Mouse EDTA Plasma (n=4) 92 78-105
Mouse Heparin Plasma (n=4) 95 81-105
Mouse Serum (n=18) 108 81-122
Rat Cell Culture Supernates (n=4) 114 103-125
Rat EDTA Plasma (n=7) 96 83-114
Rat Serum (n=10) 92 81-115

Linearity

To assess the linearity of the assay, four or more samples containing and/or spiked with high concentrations of mouse/rat Leptin in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse/Rat Leptin/OB ELISA Linearity
Mouse/Rat Leptin/OB ELISA Linearity

Data Examples

Mouse/Rat Leptin/OB ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Leptin/OB

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

Entrez Gene IDs:
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names:
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat Leptin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

110 Citations: Showing 1 - 10
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  1. Adipose Lipocalin 2 overexpression protects against age-related decline in thermogenic function of adipose tissue and metabolic deterioration
    Authors: JA Deis, H Guo, Y Wu, C Liu, DA Bernlohr, X Chen
    Mol Metab, 2019;0(0):.
    Species: Mouse
    Sample Types: Serum
  2. Sustained Intermittent Hypoxemia Induces Adiponectin Oligomers Redistribution and a Tissue-Specific Modulation of Adiponectin Receptor in Mice
    Authors: M Pierard, A Tassin, S Conotte, K Zouaoui Bo, A Legrand
    Front Physiol, 2019;10(0):68.
    Species: Mouse
    Sample Types: Plasma
  3. High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus
    Authors: E ?ebrowska, M Maciejczyk, M ?endzian-P, A Zalewska, A Chabowski
    Int J Mol Sci, 2019;20(7):.
    Species: Rat
    Sample Types: Plasma
  4. Chronically reduced BDNF levels increase oxidative stress in epididymal, but not subcutaneous, adipose tissue following high sucrose and high fat diets fed mice
    Authors: A Bodur, ? ?nce, C Kahraman, ? Abidin, S Aydin-Abid, A Alver
    Arch. Biochem. Biophys., 2019;0(0):.
    Species: Mouse
    Sample Types: Serum
  5. Quantification of the constituents of the traditional Korea medicine, Samryeongbaekchul-san, and assessment of its antiadipogenic effect
    Authors: CS Seo, SR Yoo, SJ Jeong, H Ha
    Saudi Pharm J, 2019;27(1):145-153.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Intermittent hypoxia suppression of growth hormone and insulin-like growth factor-I in the neonatal rat liver
    Authors: C Cai, T Ahmad, GB Valencia, JV Aranda, J Xu, KD Beharry
    Growth Horm. IGF Res., 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  7. Cardiac leptin overexpression in the context of acute MI and reperfusion potentiates myocardial remodeling and left ventricular dysfunction
    Authors: D Kain, AJ Simon, A Greenberg, D Ben Zvi, B Gilburd, J Schneiderm
    PLoS ONE, 2018;13(10):e0203902.
    Species: Mouse
    Sample Types: Plasma
  8. The antidepressant fluoxetine acts on energy balance and leptin sensitivity via BDNF
    Authors: G Scabia, I Barone, M Mainardi, G Ceccarini, M Scali, E Buzzigoli, A Dattilo, P Vitti, A Gastaldell, F Santini, T Pizzorusso, L Maffei, M Maffei
    Sci Rep, 2018;8(1):1781.
    Species: Mouse
    Sample Types: Plasma
  9. Nrf2 prevents Notch-induced insulin resistance and tumorigenesis in mice
    Authors: DV Chartoumpe, Y Yagishita, M Fazzari, DL Palliyagur, UN Rao, A Zaravinos, NK Khoo, FJ Schopfer, KR Weiss, GK Michalopou, I Sipula, RM O'Doherty, TW Kensler, N Wakabayash
    JCI Insight, 2018;3(5):.
    Species: Mouse
    Sample Types: Plasma
  10. Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
    Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
    Mol. Cancer Ther., 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  11. Deletion of the cold thermoreceptor TRPM8 increases heat loss and food intake leading to reduced body temperature and obesity in mice
    Authors: A Reimúndez, C Fernández-, G García, R Fernández, P Ordás, R Gallego, JL Pardo-Vazq, V Arce, F Viana, R Señarís
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Serum
  12. Leptin Increases Particle-Induced Osteolysis in Female ob/ob Mice
    Authors: KA Philbrick, AJ Branscum, CP Wong, RT Turner, UT Iwaniec
    Sci Rep, 2018;8(1):14790.
    Species: Mouse
    Sample Types: Serum
  13. Hesperidin and capsaicin, but not the combination, prevent hepatic steatosis and other metabolic syndrome-related alterations in western diet-fed rats
    Authors: A Mosqueda-S, J Sánchez, B Reynés, M Palou, MP Portillo, A Palou, C Picó
    Sci Rep, 2018;8(1):15100.
    Species: Rat
    Sample Types: Plasma
  14. Baicalin Exerts Anti-Airway Inflammation and Anti-Remodelling Effects in Severe Stage Rat Model of Chronic Obstructive Pulmonary Disease
    Authors: G Wang, N Mohammadtu, Y Lv, H Zhang, J Sun, J Dong
    Evid Based Complement Alternat Med, 2018;2018(0):7591348.
    Species: Rat
    Sample Types: Serum
  15. The exposure to uteroplacental insufficiency is associated with activation of unfolded protein response in postnatal life
    Authors: A Deodati, J Argemí, D Germani, A Puglianiel, A Alisi, C De Stefani, R Ferrero, V Nobili, T Aragón, S Cianfarani
    PLoS ONE, 2018;13(6):e0198490.
    Species: Rat
    Sample Types: Plasma
  16. Loss of Nucleobindin-2 Causes Insulin Resistance in Obesity without Impacting Satiety or Adiposity
    Authors: A Ravussin, YH Youm, J Sander, S Ryu, K Nguyen, L Varela, GI Shulman, S Sidorov, TL Horvath, JL Schultze, VD Dixit
    Cell Rep, 2018;24(5):1085-1092.e6.
    Species: Mouse
    Sample Types: Serum
  17. Disentangling Host-Microbiota Regulation of Lipid Secretion by Enterocytes: Insights from Commensals Lactobacillus paracasei and Escherichia coli
    Authors: A Tazi, JR Araujo, C Mulet, ET Arena, G Nigro, T Pédron, PJ Sansonetti
    MBio, 2018;9(5):.
    Species: Mouse
    Sample Types: Serum
  18. Specific Features of the Hypothalamic Leptin Signaling Response to Cold Exposure Are Reflected in Peripheral Blood Mononuclear Cells in Rats and Ferrets
    Authors: B Reynés, M Klein Haze, E García-Rui, J Keijer, P Oliver, A Palou
    Front Physiol, 2017;8(0):581.
    Species: Rat
    Sample Types: Serum
  19. Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes
    Authors: M Rödiger, MW Werno, I Wilhelmi, C Baumeier, D Hesse, N Wettschure, S Offermanns, K Song, M Krau beta, A Schürmann
    Mol Metab, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Effects of epigallocatechin-3-gallate on thermogenesis and mitochondrial biogenesis in brown adipose tissues of diet-induced obese mice
    Authors: MS Lee, Y Shin, S Jung, Y Kim
    Food Nutr Res, 2017;61(1):1325307.
    Species: Mouse
    Sample Types: Plasma
  21. Leptin-induced basal Akt phosphorylation and its implication in exercise-mediated improvement of insulin sensitivity
    Authors: X Zheng, S Niu
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Rat
    Sample Types: Serum
  22. Voluntary physical activity counteracts the proliferative tumor growth microenvironment created by adipose tissue via high-fat diet feeding in female rats
    Authors: CF Theriau, MK Connor
    Physiol Rep, 2017;5(13):.
    Species: Rat
    Sample Types: Tissue Culture Supernates
  23. The SGLT2 inhibitor empagliflozin improves the primary diabetic complications in ZDF rats
    Authors: S Steven, M Oelze, A Hanf, S Kröller-Sc, F Kashani, S Roohani, P Welschof, M Kopp, U Gödtel-Arm, N Xia, H Li, E Schulz, KJ Lackner, L Wojnowski, SP Bottari, P Wenzel, E Mayoux, T Münzel, A Daiber
    Redox Biol, 2017;13(0):370-385.
    Species: Rat
    Sample Types: Serum
  24. Weight Perturbation Alters Leptin Signal Transduction in a Region-Specific Manner throughout the Brain
    Authors: MV Morabito, Y Ravussin, BR Mueller, AA Skowronski, K Watanabe, KS Foo, SX Lee, A Lehmann, S Hjorth, LM Zeltser, CA LeDuc, RL Leibel
    PLoS ONE, 2017;12(1):e0168226.
    Species: Mouse
    Sample Types: Serum
  25. Effects of mild calorie restriction on lipid metabolism and inflammation in liver and adipose tissue
    Authors: CY Park, S Park, MS Kim, HK Kim, SN Han
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  26. Effects of Low-Dose Developmental Bisphenol A Exposure on Metabolic Parameters and Gene Expression in Male and Female Fischer 344 Rat Offspring
    Authors: MH Lejonklou, L Dunder, E Bladin, V Pettersson, M Rönn, L Lind, TB Waldén, PM Lind
    Environ. Health Perspect., 2017;125(6):067018.
    Species: Rat
    Sample Types: Plasma
  27. Prolonged high-fat diet induces gradual and fat depot-specific DNA methylation changes in adult mice
    Authors: RA Zwamborn, RC Slieker, PC Mulder, I Zoetemelk, L Verschuren, HE Suchiman, KH Toet, S Droog, PE Slagboom, T Kooistra, R Kleemann, BT Heijmans
    Sci Rep, 2017;7(0):43261.
    Species: Mouse
    Sample Types: Plasma
  28. High-fat diet-induced obesity and insulin resistance are characterized by differential beta oscillatory signaling of the limbic cortico-basal ganglia loop
    Authors: L Maurer, H Tang, JK Haumesser, J Altschüler, AA Kühn, J Spranger, C van Riesen
    Sci Rep, 2017;7(1):15555.
    Species: Mouse
    Sample Types: Serum
  29. Adipose Tissue Deficiency of Hormone-Sensitive Lipase Causes Fatty Liver in Mice
    Authors: B Xia, GH Cai, H Yang, SP Wang, GA Mitchell, JW Wu
    PLoS Genet., 2017;13(12):e1007110.
    Species: Mouse
    Sample Types: Plasma
  30. ASK1 (MAP3K5) is transcriptionally upregulated by E2F1 in adipose tissue in obesity, molecularly defining a human dys-metabolic obese phenotype
    Authors: Y Haim, M Blüher, D Konrad, N Goldstein, N Klöting, I Harman-Boe, B Kirshtein, D Ginsberg, T Tarnovscki, Y Gepner, I Shai, A Rudich
    Mol Metab, 2017;6(7):725-736.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Further investigation of phenotypes and confounding factors of progressive ratio performance and feeding behavior in the BACHD rat model of Huntington disease
    Authors: EK Clemensson, LE Clemensson, B Fabry, O Riess, HP Nguyen
    PLoS ONE, 2017;12(3):e0173232.
    Species: Rat
    Sample Types: Serum
  32. Sterol regulatory element-binding protein-1c orchestrates metabolic remodeling of white adipose tissue by caloric restriction
    Authors: N Fujii, T Narita, N Okita, M Kobayashi, Y Furuta, Y Chujo, M Sakai, A Yamada, K Takeda, T Konishi, Y Sudo, I Shimokawa, Y Higami
    Aging Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Plasma
  33. Elevated hepatic DPP4 activity promotes insulin resistance and non-alcoholic fatty liver disease
    Authors: C Baumeier, L Schlüter, S Saussentha, T Laeger, M Rödiger, SA Alaze, L Fritsche, HU Häring, N Stefan, A Fritsche, RW Schwenk, A Schürmann
    Mol Metab, 2017;6(10):1254-1263.
    Species: Mouse
    Sample Types: Plasma
  34. Monocyte chemotactic protein-1 deficiency reduces spontaneous metastasis of Lewis lung carcinoma in mice fed a high-fat diet
    Oncotarget, 2016;7(17):24792-9.
    Species: Mouse
    Sample Types: Plasma
  35. Korean Curcuma longa L. induces lipolysis and regulates leptin in adipocyte cells and rats
    Nutr Res Pract, 2016;10(5):487-493.
  36. Reprogramming the body weight set point by a reciprocal interaction of hypothalamic leptin sensitivity and Pomc gene expression reverts extreme obesity
    Mol Metab, 2016;5(10):869-81.
    Species: Mouse
    Sample Types: Plasma
  37. Is Western Diet-Induced Nonalcoholic Steatohepatitis in Ldlr-/- Mice Reversible?
    Authors: Lytle K, Jump D
    PLoS ONE, 2016;11(1):e0146942.
    Species: Mouse
    Sample Types: Plasma
  38. Deficiency in prohormone convertase PC1 impairs prohormone processing in Prader-Willi syndrome
    Authors: Lisa C Burnett
    J. Clin. Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: Plasma
  39. Traditional medicine, Sobokchukeo?Tang, modulates the inflammatory response in adipocytes and macrophages
    Mol Med Rep, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  40. Differential body weight, blood pressure and placental inflammatory responses to normal versus high-fat diet in melanocortin-4 receptor-deficient pregnant rats
    Authors: FT Spradley, AC Palei, JP Granger
    J. Hypertens, 2016;34(10):1998-2007.
    Species: Rat
    Sample Types: Plasma
  41. Anti-Inflammatory Effects of Rosiglitazone in Obesity-Impaired Wound Healing Depend on Adipocyte Differentiation
    PLoS ONE, 2016;11(12):e0168562.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  42. Moderate calorie restriction during gestation programs offspring for lower BAT thermogenic capacity driven by thyroid and sympathetic signaling.
    Authors: Palou M, Priego T, Romero M, Szostaczuk N, Konieczna J, Cabrer C, Remesar X, Palou A, Pico C
    Int J Obes (Lond), 2015;39(2):339-45.
    Species: Rat
    Sample Types: Plasma
  43. A spontaneous deletion within the desmoglein 3 extracellular domain of mice results in hypomorphic protein expression, immunodeficiency, and a wasting disease phenotype.
    Authors: Kountikov E, Poe J, Maclver N, Rathmell J, Tedder T
    Am J Pathol, 2015;185(3):617-30.
    Species: Mouse
    Sample Types: Serum
  44. Leptin ameliorates ischemic necrosis of the femoral head in rats with obesity induced by a high-fat diet.
    Authors: Zhou L, Jang K, Moon Y, Wagle S, Kim K, Lee K, Park B, Kim J
    Sci Rep, 2015;5(0):9397.
    Species: Rat
    Sample Types: Plasma
  45. Modulation of the leptin receptor mediates tumor growth and migration of pancreatic cancer cells.
    Authors: Mendonsa A, Chalfant M, Gorden L, VanSaun M
    PLoS ONE, 2015;10(4):e0126686.
    Species: Mouse
    Sample Types: Plasma
  46. T-cell costimulation protects obesity-induced adipose inflammation and insulin resistance.
    Authors: Zhong, Jixin, Rao, Xiaoquan, Braunstein, Zachary, Taylor, Anne, Narula, Vimal, Hazey, Jeffrey, Mikami, Dean, Needleman, Bradley, Rutsky, Jessica, Sun, Qinghua, Deiuliis, Jeffrey, Satoskar, Abhay R, Rajagopalan, Sanjay
    Diabetes, 2014;63(4):1289-302.
    Species: Mouse
    Sample Types: Serum
  47. Beneficial metabolic effects of rapamycin are associated with enhanced regulatory cells in diet-induced obese mice.
    Authors: Makki K, Taront S, Molendi-Coste O, Bouchaert E, Neve B, Eury E, Lobbens S, Labalette M, Duez H, Staels B, Dombrowicz D, Froguel P, Wolowczuk I
    PLoS ONE, 2014;9(4):e92684.
    Species: Mouse
    Sample Types: Serum
  48. Leptin metabolically licenses T cells for activation to link nutrition and immunity.
    Authors: Saucillo, Donte C, Gerriets, Valerie, Sheng, John, Rathmell, Jeffrey, Maciver, Nancie J
    J Immunol, 2014;192(1):136-44.
    Species: Mouse
    Sample Types: Serum
  49. Obesity induced a leptin-Notch signaling axis in breast cancer.
    Authors: Battle M, Gillespie C, Quarshie A, Lanier V, Harmon T, Wilson K, Torroella-Kouri M, Gonzalez-Perez R
    Int J Cancer, 2014;134(7):1605-16.
    Species: Mouse
    Sample Types: Plasma
  50. Rescue of cardiac leptin receptors in db/db mice prevents myocardial triglyceride accumulation.
    Authors: Hall M, Maready M, Hall J, Stec D
    Am J Physiol Endocrinol Metab, 2014;307(3):E316-25.
    Species: Mouse
    Sample Types: Plasma
  51. Ghrelin induces leptin resistance by activation of suppressor of cytokine signaling 3 expression in male rats: implications in satiety regulation.
    Authors: Heldsinger A, Grabauskas G, Wu X, Zhou S, Lu Y, Song I, Owyang C
    Endocrinology, 2014;155(10):3956-69.
    Species: Rat
    Sample Types: Plasma
  52. A leptin-mediated central mechanism in analgesia-enhanced opioid reward in rats.
    Authors: Lim G, Kim H, McCabe M, Chou C, Wang S, Chen L, Marota J, Blood A, Breiter H, Mao J
    J Neurosci, 2014;34(29):9779-88.
    Species: Rat
    Sample Types: Serum
  53. Transgenic mice overexpressing amyloid precursor protein exhibit early metabolic deficits and a pathologically low leptin state associated with hypothalamic dysfunction in arcuate neuropeptide Y neurons.
    Authors: Ishii M, Wang G, Racchumi G, Dyke J, Iadecola C
    J Neurosci, 2014;34(27):9096-106.
    Species: Mouse
    Sample Types: Plasma
  54. Butter naturally enriched in cis-9, trans-11 CLA prevents hyperinsulinemia and increases both serum HDL cholesterol and triacylglycerol levels in rats.
    Authors: de Almeida M, Luquetti S, Sabarense C, do Amaral Correa J, dos Reis L, Santos da Conceicao E, Lisboa P, de Moura E, Gameiro J, da Gama M, Lopes F, Garcia R
    Lipids Health Dis, 2014;13(0):200.
    Species: Rat
    Sample Types: Serum
  55. Temporal changes in nutritional state affect hypothalamic POMC peptide levels independently of leptin in adult male mice.
    Authors: Mercer A, Stuart R, Attard C, Otero-Corchon V, Nillni E, Low M
    Am J Physiol Endocrinol Metab, 2014;306(8):E904-15.
    Species: Mouse
    Sample Types: Plasma
  56. Lipid profiling and transcriptomic analysis reveals a functional interplay between estradiol and growth hormone in liver.
    Authors: Fernandez-Perez L, Santana-Farre R, de Mirecki-Garrido M, Garcia I, Guerra B, Mateo-Diaz C, Iglesias-Gato D, Diaz-Chico J, Flores-Morales A, Diaz M
    PLoS ONE, 2014;9(5):e96305.
    Species: Rat
    Sample Types: Serum
  57. PKClambda haploinsufficiency prevents diabetes by a mechanism involving alterations in hepatic enzymes.
    Authors: Sajan M, Ivey R, Lee M, Mastorides S, Jurczak M, Samuels V, Shulman G, Braun U, Leitges M, Farese R
    Mol Endocrinol, 2014;28(7):1097-107.
    Species: Mouse
    Sample Types: Serum
  58. Effect of intermittent cold exposure on brown fat activation, obesity, and energy homeostasis in mice.
    Authors: Ravussin Y, Xiao C, Gavrilova O, Reitman M
    PLoS ONE, 2014;9(1):e85876.
    Species: Mouse
    Sample Types: Serum
  59. Hypermethylation of Sp1 binding site suppresses hypothalamic POMC in neonates and may contribute to metabolic disorders in adults: impact of maternal dietary CLAs.
    Authors: Zhang, Xiaomei, Yang, Ran, Jia, Yan, Cai, Demin, Zhou, Bo, Qu, Xiaoli, Han, Huihua, Xu, Liang, Wang, Linfeng, Yao, Yanan, Yang, Guoqing
    Diabetes, 2014;63(5):1475-87.
    Species: Mouse
    Sample Types: Plasma
  60. Blockade of the cerebral aqueduct in rats provides evidence of antagonistic leptin responses in the forebrain and hindbrain.
    Authors: Vaill M, Desai B, Harris R
    Am J Physiol Endocrinol Metab, 2014;306(4):E414-23.
    Species: Rat
    Sample Types: CSF
  61. Protopanaxatriol, a novel PPARgamma antagonist from Panax ginseng, alleviates steatosis in mice.
    Authors: Zhang Y, Yu L, Cai W, Fan S, Feng L, Ji G, Huang C
    Sci Rep, 2014;4(0):7375.
    Species: Mouse
    Sample Types: Serum
  62. Euglycemic hyperinsulinemia increases blood pressure in pregnant rats independent of placental antiangiogenic and inflammatory factors.
    Authors: Palei A, Spradley F, Granger J
    Am J Hypertens, 2013;26(12):1445-51.
    Species: Rat
    Sample Types: Serum
  63. Oral leptin treatment in suckling rats ameliorates detrimental effects in hypothalamic structure and function caused by maternal caloric restriction during gestation.
    Authors: Konieczna J, Garcia A, Sanchez J, Palou M, Palou A, Pico C
    PLoS ONE, 2013;8(11):e81906.
    Species: Rat
    Sample Types: Plasma
  64. Enhanced leptin sensitivity, reduced adiposity, and improved glucose homeostasis in mice lacking exchange protein directly activated by cyclic AMP isoform 1.
    Authors: Yan J, Mei F, Cheng H, Lao D, Hu Y, Wei J, Patrikeev I, Hao D, Stutz S, Dineley K, Motamedi M, Hommel J, Cunningham K, Chen J, Cheng X
    Mol Cell Biol, 2013;33(5):918-26.
    Species: Mouse
    Sample Types: Plasma
  65. Obesity and IL-6 interact in modulating the response to endotoxemia in mice.
    Cytokine, 2013;61(1):71-7.
    Species: Mouse
    Sample Types: Plasma
  66. HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.
    Authors: Gautier E, Westerterp M, Bhagwat N, Cremers S, Shih A, Abdel-Wahab O, Lutjohann D, Randolph G, Levine R, Tall A, Yvan-Charvet L
    J Exp Med, 2013;210(2):339-53.
    Species: Mouse
    Sample Types: Plasma
  67. Exercise protects against diet-induced insulin resistance through downregulation of protein kinase Cbeta in mice.
    Authors: Rao X, Zhong J, Xu X, Jordan B, Maurya S, Braunstein Z, Wang T, Huang W, Aggarwal S, Periasamy M, Rajagopalan S, Mehta K, Sun Q
    PLoS ONE, 2013;8(12):e81364.
    Species: Mouse
    Sample Types: Plasma
  68. Disruption of the chemokine-like receptor-1 (CMKLR1) gene is associated with reduced adiposity and glucose intolerance.
    Authors: Ernst MC, Haidl ID, Zúñiga LA, Dranse HJ, Rourke JL, Zabel BA, Butcher EC, Sinal CJ
    Endocrinology, 2012;153(2):672-82.
    Species: Mouse
    Sample Types: Serum
  69. Tangeretin stimulates glucose uptake via regulation of AMPK signaling pathways in C2C12 myotubes and improves glucose tolerance in high-fat diet-induced obese mice.
    Mol. Cell. Endocrinol., 2012;358(1):127-34.
    Species: Mouse
    Sample Types: Serum
  70. HCV core-induced nonobese hepatic steatosis is associated with hypoadiponectinemia and is ameliorated by adiponectin administration.
    Authors: Chang ML, Yeh HC, Tsou YK
    Obesity (Silver Spring), 2012;20(7):1474-80.
    Species: Mouse
    Sample Types: Serum
  71. Obesity in mice with adipocyte-specific deletion of clock component Arntl.
    Authors: Paschos, Georgios, Ibrahim, Salam, Song, Wen-Lian, Kunieda, Takeshig, Grant, Gregory, Reyes, Teresa M, Bradfield, Christop, Vaughan, Cheryl H, Eiden, Michael, Masoodi, Mojgan, Griffin, Julian L, Wang, Fenfen, Lawson, John A, Fitzgerald, Garret A
    Nat Med, 2012;18(12):1768-77.
    Species: Mouse
    Sample Types: Plasma
  72. Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function.
    Authors: Sato H, Isogai Y, Masuda S
    J. Biol. Chem., 2011;286(13):11632-48.
    Species: Mouse
    Sample Types: Serum
  73. Fasting induces an anti-inflammatory effect on the neuroimmune system which a high-fat diet prevents.
    Authors: Lavin DN, Joesting JJ, Chiu GS, Moon ML, Meng J, Dilger RN, Freund GG
    Obesity (Silver Spring), 2011;19(8):1586-94.
    Species: Mouse
    Sample Types: Serum
  74. Castration-induced changes in mouse epididymal white adipose tissue.
    Authors: Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S, Thompson TC
    Mol. Cell. Endocrinol., 2011;345(1):58-67.
    Species: Mouse
    Sample Types: Serum
  75. Perinatal lack of maternal IL-6 promotes increased adiposity during adulthood in mice.
    Authors: Lager S, Asterholm IW, Schele E, Jansson N, Nilsson S, Jansson JO, Lonn M, Holmang A
    Endocrinology, 2011;152(4):1336-46.
    Species: Mouse
    Sample Types: Milk
  76. Enhanced production of IL-17A during zymosan-induced peritonitis in obese mice.
    Authors: Pini M, Fantuzzi G
    J. Leukoc. Biol., 2010;87(1):51-8.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  77. Leptin exacerbates sepsis-mediated morbidity and mortality.
    Authors: Shapiro NI, Khankin EV, van Meurs M
    J. Immunol., 2010;185(1):517-24.
    Species: Mouse
    Sample Types: Plasma
  78. eNOS plays a major role in adiponectin synthesis in adipocytes.
    Authors: Koh EH, Kim M, Ranjan KC, Kim HS, Park HS, Oh KS, Park IS, Lee WJ, Kim MS, Park JY, Youn JH, Lee KU
    Am. J. Physiol. Endocrinol. Metab., 2010;298(4):E846-53.
    Species: Mouse
    Sample Types: Plasma
  79. Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
    Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
    Cytokine, 2010;49(3):256-63.
    Species: Mouse
    Sample Types: BALF
  80. Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance.
    Authors: Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM
    Diabetes, 2010;59(4):935-46.
    Species: Mouse
    Sample Types: Plasma
  81. Deletion of Nhlh2 results in a defective torpor response and reduced Beta adrenergic receptor expression in adipose tissue.
    Authors: Wankhade UD, Vella KR, Fox DL
    PLoS ONE, 2010;5(8):e12324.
    Species: Mouse
    Sample Types: Serum
  82. Group X secretory phospholipase A2 negatively regulates adipogenesis in murine models.
    Authors: Li X, Shridas P, Forrest K, Bailey W, Webb NR
    FASEB J., 2010;24(11):4313-24.
    Species: Mouse
    Sample Types: Plasma
  83. Differential effects of high MUFA with high or low P/S ratio (polyunsaturated to saturated fatty acids) on improving hepatic lipolytic enzymes and mediating PPARgamma related with lipoprotein lipase and hormone-sensitive lipase of white adipose tissue in diet-induced obese hamster.
    Authors: Liao FH, Liou TH, Chiu WC, Shieh MJ, Chien YW
    Int J Obes (Lond), 2010;34(11):1608-17.
    Species: Hamster
    Sample Types: Plasma
  84. Moderate caloric restriction in lactating rats protects offspring against obesity and insulin resistance in later life.
    Authors: Palou M, Priego T, Sanchez J, Torrens JM, Palou A, Pico C
    Endocrinology, 2010;151(3):1030-41.
    Species: Rat
    Sample Types: Milk
  85. Development of obesity in the Otsuka Long-Evans Tokushima Fatty rat.
    Authors: Schroeder M, Zagoory-Sharon O, Shbiro L, Marco A, Hyun J, Moran TH, Bi S, Weller A
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009;297(6):R1749-60.
    Species: Rat
    Sample Types: Plasma
  86. Stearoyl-CoA desaturase 1 deficiency protects mice from immune-mediated liver injury.
    Authors: Feng D, Wang Y, Mei Y, Xu Y, Xu H, Lu Y, Luo Q, Zhou S, Kong X, Xu L
    Lab. Invest., 2009;89(2):222-30.
    Species: Mouse
    Sample Types: Plasma
  87. Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
    Authors: Lim G, Wang S, Zhang Y, Tian Y, Mao J
    J. Clin. Invest., 2009;119(2):295-304.
    Species: Rat
    Sample Types: Plasma
  88. Efficient method for obtaining Lep(ob)/Lep(ob)-derived animal models using adipose tissue transplantations.
    Authors: Barros CC, Almeida SS, Mori MA, Valero VB, Haro AS, Batista EC, Rosa TS, Bacurau RF, Wurtele M, Araujo RC
    Int J Obes (Lond), 2009;33(8):938-44.
    Species: Mouse
    Sample Types: Serum
  89. Early handling increases susceptibility to experimental autoimmune encephalomyelitis (EAE) in C57BL/6 male mice.
    Authors: Columba-Cabezas S, Iaffaldano G, Chiarotti F, Alleva E, Cirulli F
    J. Neuroimmunol., 2009;212(1):10-6.
    Species: Mouse
    Sample Types: Serum
  90. Hepatic CB1 receptor is required for development of diet-induced steatosis, dyslipidemia, and insulin and leptin resistance in mice.
    Authors: Osei-Hyiaman D, Liu J, Zhou L, Godlewski G, Harvey-White J, Jeong WI, Batkai S, Marsicano G, Lutz B, Buettner C, Kunos G
    J. Clin. Invest., 2008;118(9):3160-9.
    Species: Mouse
    Sample Types: Plasma
  91. Role and regulation of adipokines during zymosan-induced peritoneal inflammation in mice.
    Authors: Pini M, Gove ME, Sennello JA, van Baal JW, Chan L, Fantuzzi G
    Endocrinology, 2008;149(8):4080-5.
    Species: Mouse
    Sample Types: Serum
  92. Identification of ALOX5 as a gene regulating adiposity and pancreatic function.
    Authors: Mehrabian M, Schulthess FT, Nebohacova M, Castellani LW, Zhou Z, Hartiala J, Oberholzer J, Lusis AJ, Maedler K, Allayee H
    Diabetologia, 2008;51(6):978-88.
    Species: Mouse
    Sample Types: Plasma
  93. Signal transducer and activator of transcription-3 is required in hypothalamic agouti-related protein/neuropeptide Y neurons for normal energy homeostasis.
    Authors: Gong L, Yao F, Hockman K, Heng HH, Morton GJ, Takeda K, Akira S, Low MJ, Rubinstein M, MacKenzie RG
    Endocrinology, 2008;149(7):3346-54.
    Species: Mouse
    Sample Types: Plasma
  94. Novel black soy peptides with antiobesity effects: activation of leptin-like signaling and AMP-activated protein kinase.
    Authors: Jang EH, Moon JS, Ko JH, Ahn CW, Lee HH, Shin JK, Park CS, Kang JH
    Int J Obes (Lond), 2008;32(7):1161-70.
    Species: Mouse
    Sample Types: Plasma
  95. Adaptation to lactation in OLETF rats lacking CCK-1 receptors: body weight, fat tissues, leptin and oxytocin.
    Authors: Zagoory-Sharon O, Schroeder M, Levine A, Moran TH, Weller A
    Int J Obes (Lond), 2008;32(8):1211-21.
    Species: Rat
    Sample Types: Plasma
  96. Doubly truncated FosB isoform (Delta2DeltaFosB) induces osteosclerosis in transgenic mice and modulates expression and phosphorylation of Smads in osteoblasts independent of intrinsic AP-1 activity.
    Authors: Sabatakos G, Rowe GC, Kveiborg M, Wu M, Neff L, Chiusaroli R, Philbrick WM, Baron R
    J. Bone Miner. Res., 2008;23(5):584-95.
    Species: Mouse
    Sample Types: Serum
  97. Octylphenol stimulates resistin gene expression in 3T3-L1 adipocytes via the estrogen receptor and extracellular signal-regulated kinase pathways.
    Authors: Lee MJ, Lin H, Liu CW, Wu MH, Liao WJ, Chang HH, Ku HC, Chien YS, Ding WH, Kao YH
    Am. J. Physiol., Cell Physiol., 2008;294(6):C1542-51.
    Species: Mouse
    Sample Types: Serum
  98. FXR-deficiency confers increased susceptibility to torpor.
    Authors: Cariou B, Bouchaert E, Abdelkarim M, Dumont J, Caron S, Fruchart JC, Burcelin R, Kuipers F, Staels B
    FEBS Lett., 2007;581(27):5191-8.
    Species: Mouse
    Sample Types: Plasma
  99. High circulating leptin receptors with normal leptin sensitivity in liver-specific insulin receptor knock-out (LIRKO) mice.
    Authors: Cohen SE, Kokkotou E, Biddinger SB, Kondo T, Gebhardt R, Kratzsch J, Mantzoros CS, Kahn CR
    J. Biol. Chem., 2007;282(32):23672-8.
    Species: Mouse
    Sample Types: Serum
  100. Leptin induces elongation of cardiac myocytes and causes eccentric left ventricular dilatation with compensation.
    Authors: Abe Y, Ono K, Kawamura T, Wada H, Kita T, Shimatsu A, Hasegawa K
    Am. J. Physiol. Heart Circ. Physiol., 2007;292(5):H2387-96.
    Species: Mouse
    Sample Types: Plasma
  101. Integrating genetic and network analysis to characterize genes related to mouse weight.
    Authors: Ghazalpour A, Doss S, Zhang B, Wang S, Plaisier C, Castellanos R, Brozell A, Schadt EE, Drake TA, Lusis AJ, Horvath S
    PLoS Genet., 2006;2(8):e130.
    Species: Mouse
    Sample Types: Plasma
  102. Impaired adipose tissue development in mice with inactivation of placental growth factor function.
    Authors: Lijnen HR, Christiaens V, Scroyen I, Voros G, Tjwa M, Carmeliet P, Collen D
    Diabetes, 2006;55(10):2698-704.
    Species: Mouse
    Sample Types: Tissue Homogenates
  103. Leptin resistance protects mice from hyperoxia-induced acute lung injury.
    Authors: Bellmeyer A, Martino JM, Chandel NS, Scott Budinger GR, Dean DA, Mutlu GM
    Am. J. Respir. Crit. Care Med., 2006;175(6):587-94.
    Species: Mouse
    Sample Types: BALF
  104. Metformin restores leptin sensitivity in high-fat-fed obese rats with leptin resistance.
    Authors: Kim YW, Kim JY, Park YH, Park SY, Won KC, Choi KH, Huh JY, Moon KH
    Diabetes, 2006;55(3):716-24.
    Species: Rat
    Sample Types: CSF
  105. Long-term blockade of angiotensin AT1 receptors increases survival of obese Zucker rats.
    Authors: O&amp;amp;apos;Connor SE
    Eur. J. Pharmacol., 2006;534(1):271-9.
    Species: Mouse
    Sample Types: Plasma
  106. Diurnal rhythms of leptin and ghrelin in the systemic circulation and in the gastric mucosa are related to food intake in rats.
    Authors: Sanchez J, Oliver P, Pico C, Palou A
    Pflugers Arch., 2004;448(5):500-6.
    Species: Rat
    Sample Types: Serum
  107. Role of leptin deficiency in early acute renal failure during endotoxemia in ob/ob mice.
    Authors: Wang W, Poole B, Mitra A, Falk S, Fantuzzi G, Lucia S, Schrier R
    J. Am. Soc. Nephrol., 2004;15(3):645-9.
    Species: Mouse
    Sample Types: Serum
  108. Leptin accelerates autoimmune diabetes in female NOD mice.
    Authors: Matarese G, Sanna V, Lechler RI, Sarvetnick N, Fontana S, Zappacosta S, La Cava A
    Diabetes, 2002;51(5):1356-61.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  109. The increased bone mass in deltaFosB transgenic mice is independent of circulating leptin levels.
    Authors: Kveiborg M, Chiusaroli R, Sims NA, Wu M, Sabatakos G, Horne WC, Baron R
    Endocrinology, 2002;143(11):4304-9.
    Species: Mouse
    Sample Types: Serum
  110. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity.
    Authors: Yamauchi, T, Kamon, J, Waki, H, Terauchi, Y, Kubota, N, Hara, K, Mori, Y, Ide, T, Murakami, K, Tsuboyama-Kasaoka, N, Ezaki, O, Akanuma, Y, Gavrilova, O, Vinson, C, Reitman, M L, Kagechika, H, Shudo, K, Yoda, M, Nakano, Y, Tobe, K, Nagai, R, Kimura, S, Tomita, M, Froguel, P, Kadowaki, T
    Nat Med, 2001;7(8):941-6.
    Species: Mouse
    Sample Types: Plasma

FAQs

  1. Why is the acid activation procedure required for pregnant mouse serum samples from second trimester through post-partum day 1 in the Mouse/Rat Leptin Quantikine® ELISA assay?

    • Mouse Leptin R intereferes in the MOB00 assay at high concentration >2000 pg/mL, and studies have shown that there is a massive increase in the level of criculating Leptin Receptor (OB-R) during later stage of pregnancy. Acid activation helps to dissociate the interaction between Leptin and its high affinity recceptor OB-R thus minimizing possible interference in the Leptin assay. Note that activation is not required for samples obtained from non-pregnant mice. Ref. J. Biol. Chem (1997) 272(48):30546-51.

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Mouse/Rat Leptin Quantikine ELISA Kit
By Anonymous on 04/03/2018
Sample Tested: Serum

Mouse/Rat Leptin Quantikine ELISA Kit
By Anonymous on 06/05/2017
Sample Tested: 7TD1 mouse hybridoma cell line

Mouse/Rat Leptin Quantikine ELISA Kit
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Sample Tested: Mouse/Rat Leptin Elisa Kit

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