Mouse/Rat Leptin Quantikine ELISA Kit

(61 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL)
  • Sensitivity
    22 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse and rat Leptin.  This assay cross-reacts 0.24% with Human Leptin.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse Leptin Immunoassay is a 4.5 hour solid phase ELISA designed to measure soluble mouse leptin in cell culture supernates, serum*, and plasma. It contains recombinant mouse leptin expressed in E. coli and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse leptin accurately. Results obtained measuring natural mouse leptin showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse leptin.

*Pregnant mouse serum from the second trimester of pregnancy through post-partum day 1 require acid/urea activation of mouse leptin.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation7.922.667.914.230.587.4


The recovery of mouse/rat Leptin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=5) 101 85-120
Mouse EDTA Plasma (n=4) 92 78-105
Mouse Heparin Plasma (n=4) 95 81-105
Mouse Serum (n=18) 108 81-122
Rat Cell Culture Supernates (n=4) 114 103-125
Rat EDTA Plasma (n=7) 96 83-114
Rat Serum (n=10) 92 81-115
To assess the linearity of the assay, four or more samples containing and/or spiked with high concentrations of mouse/rat Leptin in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse/Rat Leptin Quantikine ELISA Kit
Mouse/Rat Leptin Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Leptin/OB
Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.
    • Entrez Gene IDs
      3952 (Human); 16846 (Mouse); 25608 (Rat);
    • Alternate Names
      FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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    Sample Type
    1. Is Western Diet-Induced Nonalcoholic Steatohepatitis in Ldlr-/- Mice Reversible?
      Authors: Lytle K, Jump D
      PLoS ONE, 2016;11(1):e0146942.
      Species: Mouse
      Sample Type: Plasma
    2. Leptin ameliorates ischemic necrosis of the femoral head in rats with obesity induced by a high-fat diet.
      Authors: Zhou L, Jang K, Moon Y, Wagle S, Kim K, Lee K, Park B, Kim J
      Sci Rep, 2015;5(0):9397.
      Species: Rat
      Sample Type: Plasma
    3. Moderate calorie restriction during gestation programs offspring for lower BAT thermogenic capacity driven by thyroid and sympathetic signaling.
      Authors: Palou M, Priego T, Romero M, Szostaczuk N, Konieczna J, Cabrer C, Remesar X, Palou A, Pico C
      Int J Obes (Lond), 2015;39(2):339-45.
      Species: Rat
      Sample Type: Plasma
    4. Obesity induced a leptin-Notch signaling axis in breast cancer.
      Authors: Battle M, Gillespie C, Quarshie A, Lanier V, Harmon T, Wilson K, Torroella-Kouri M, Gonzalez-Perez R
      Int J Cancer, 2014;134(7):1605-16.
      Species: Mouse
      Sample Type: Plasma
    5. Butter naturally enriched in cis-9, trans-11 CLA prevents hyperinsulinemia and increases both serum HDL cholesterol and triacylglycerol levels in rats.
      Authors: de Almeida M, Luquetti S, Sabarense C, do Amaral Correa J, dos Reis L, Santos da Conceicao E, Lisboa P, de Moura E, Gameiro J, da Gama M, Lopes F, Garcia R
      Lipids Health Dis, 2014;13(0):200.
      Species: Rat
      Sample Type: Serum
    6. Effect of intermittent cold exposure on brown fat activation, obesity, and energy homeostasis in mice.
      Authors: Ravussin Y, Xiao C, Gavrilova O, Reitman M
      PLoS ONE, 2014;9(1):e85876.
      Species: Mouse
      Sample Type: Serum
    7. Leptin metabolically licenses T cells for activation to link nutrition and immunity.
      Authors: Saucillo, Donte C, Gerriets, Valerie, Sheng, John, Rathmell, Jeffrey, Maciver, Nancie J
      J Immunol, 2014;192(1):136-44.
      Species: Mouse
      Sample Type: Serum
    8. Transgenic mice overexpressing amyloid precursor protein exhibit early metabolic deficits and a pathologically low leptin state associated with hypothalamic dysfunction in arcuate neuropeptide Y neurons.
      Authors: Ishii M, Wang G, Racchumi G, Dyke J, Iadecola C
      J Neurosci, 2014;34(27):9096-106.
      Species: Mouse
      Sample Type: Plasma
    9. Temporal changes in nutritional state affect hypothalamic POMC peptide levels independently of leptin in adult male mice.
      Authors: Mercer A, Stuart R, Attard C, Otero-Corchon V, Nillni E, Low M
      Am J Physiol Endocrinol Metab, 2014;306(8):E904-15.
      Species: Mouse
      Sample Type: Plasma
    10. Beneficial metabolic effects of rapamycin are associated with enhanced regulatory cells in diet-induced obese mice.
      Authors: Makki K, Taront S, Molendi-Coste O, Bouchaert E, Neve B, Eury E, Lobbens S, Labalette M, Duez H, Staels B, Dombrowicz D, Froguel P, Wolowczuk I
      PLoS ONE, 2014;9(4):e92684.
      Species: Mouse
      Sample Type: Serum
    11. Rescue of cardiac leptin receptors in db/db mice prevents myocardial triglyceride accumulation.
      Authors: Hall M, Maready M, Hall J, Stec D
      Am J Physiol Endocrinol Metab, 2014;307(3):E316-25.
      Species: Mouse
      Sample Type: Plasma
    12. Protopanaxatriol, a novel PPARgamma antagonist from Panax ginseng, alleviates steatosis in mice.
      Authors: Zhang Y, Yu L, Cai W, Fan S, Feng L, Ji G, Huang C
      Sci Rep, 2014;4(0):7375.
      Species: Mouse
      Sample Type: Serum
    13. Euglycemic hyperinsulinemia increases blood pressure in pregnant rats independent of placental antiangiogenic and inflammatory factors.
      Authors: Palei A, Spradley F, Granger J
      Am J Hypertens, 2013;26(12):1445-51.
      Species: Rat
      Sample Type: Serum
    14. Oral leptin treatment in suckling rats ameliorates detrimental effects in hypothalamic structure and function caused by maternal caloric restriction during gestation.
      Authors: Konieczna J, Garcia A, Sanchez J, Palou M, Palou A, Pico C
      PLoS ONE, 2013;8(11):e81906.
      Species: Rat
      Sample Type: Plasma
    15. Exercise protects against diet-induced insulin resistance through downregulation of protein kinase Cbeta in mice.
      Authors: Rao X, Zhong J, Xu X, Jordan B, Maurya S, Braunstein Z, Wang T, Huang W, Aggarwal S, Periasamy M, Rajagopalan S, Mehta K, Sun Q
      PLoS ONE, 2013;8(12):e81364.
      Species: Mouse
      Sample Type: Plasma
    16. Enhanced leptin sensitivity, reduced adiposity, and improved glucose homeostasis in mice lacking exchange protein directly activated by cyclic AMP isoform 1.
      Authors: Yan J, Mei F, Cheng H, Lao D, Hu Y, Wei J, Patrikeev I, Hao D, Stutz S, Dineley K, Motamedi M, Hommel J, Cunningham K, Chen J, Cheng X
      Mol Cell Biol, 2013;33(5):918-26.
      Species: Mouse
      Sample Type: Plasma
    17. HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.
      Authors: Gautier E, Westerterp M, Bhagwat N, Cremers S, Shih A, Abdel-Wahab O, Lutjohann D, Randolph G, Levine R, Tall A, Yvan-Charvet L
      J Exp Med, 2013;210(2):339-53.
      Species: Mouse
      Sample Type: Plasma
    18. Obesity and IL-6 interact in modulating the response to endotoxemia in mice.
      Cytokine, 2013;61(1):71-7.
      Species: Mouse
      Sample Type: Plasma
    19. HCV core-induced nonobese hepatic steatosis is associated with hypoadiponectinemia and is ameliorated by adiponectin administration.
      Authors: Chang ML, Yeh HC, Tsou YK
      Obesity (Silver Spring), 2012;20(7):1474-80.
      Species: Mouse
      Sample Type: Serum
    20. Tangeretin stimulates glucose uptake via regulation of AMPK signaling pathways in C2C12 myotubes and improves glucose tolerance in high-fat diet-induced obese mice.
      Mol. Cell. Endocrinol., 2012;358(1):127-34.
      Species: Mouse
      Sample Type: Serum
    21. Disruption of the chemokine-like receptor-1 (CMKLR1) gene is associated with reduced adiposity and glucose intolerance.
      Authors: Ernst MC, Haidl ID, Zúñiga LA, Dranse HJ, Rourke JL, Zabel BA, Butcher EC, Sinal CJ
      Endocrinology, 2012;153(2):672-82.
      Species: Mouse
      Sample Type: Serum
    22. Obesity in mice with adipocyte-specific deletion of clock component Arntl.
      Authors: Paschos, Georgios, Ibrahim, Salam, Song, Wen-Lian, Kunieda, Takeshig, Grant, Gregory, Reyes, Teresa M, Bradfield, Christop, Vaughan, Cheryl H, Eiden, Michael, Masoodi, Mojgan, Griffin, Julian L, Wang, Fenfen, Lawson, John A, Fitzgerald, Garret A
      Nat Med, 2012;18(12):1768-77.
      Species: Mouse
      Sample Type: Plasma
    23. Castration-induced changes in mouse epididymal white adipose tissue.
      Authors: Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S, Thompson TC
      Mol. Cell. Endocrinol., 2011;345(1):58-67.
      Species: Mouse
      Sample Type: Serum
    24. Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function.
      Authors: Sato H, Isogai Y, Masuda S
      J. Biol. Chem., 2011;286(13):11632-48.
      Species: Mouse
      Sample Type: Serum
    25. Perinatal lack of maternal IL-6 promotes increased adiposity during adulthood in mice.
      Authors: Lager S, Asterholm IW, Schele E, Jansson N, Nilsson S, Jansson JO, Lonn M, Holmang A
      Endocrinology, 2011;152(4):1336-46.
      Species: Mouse
      Sample Type: Milk
    26. Fasting induces an anti-inflammatory effect on the neuroimmune system which a high-fat diet prevents.
      Authors: Lavin DN, Joesting JJ, Chiu GS, Moon ML, Meng J, Dilger RN, Freund GG
      Obesity (Silver Spring), 2011;19(8):1586-94.
      Species: Mouse
      Sample Type: Serum
    27. Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
      Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
      Cytokine, 2010;49(3):256-63.
      Species: Mouse
      Sample Type: BALF
    28. Enhanced production of IL-17A during zymosan-induced peritonitis in obese mice.
      Authors: Pini M, Fantuzzi G
      J. Leukoc. Biol., 2010;87(1):51-8.
      Species: Mouse
      Sample Type: Peritoneal Lavage
    29. eNOS plays a major role in adiponectin synthesis in adipocytes.
      Authors: Koh EH, Kim M, Ranjan KC, Kim HS, Park HS, Oh KS, Park IS, Lee WJ, Kim MS, Park JY, Youn JH, Lee KU
      Am. J. Physiol. Endocrinol. Metab., 2010;298(4):E846-53.
      Species: Mouse
      Sample Type: Plasma
    30. Leptin exacerbates sepsis-mediated morbidity and mortality.
      Authors: Shapiro NI, Khankin EV, van Meurs M
      J. Immunol., 2010;185(1):517-24.
      Species: Mouse
      Sample Type: Plasma
    31. Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance.
      Authors: Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM
      Diabetes, 2010;59(4):935-46.
      Species: Mouse
      Sample Type: Plasma
    32. Deletion of Nhlh2 results in a defective torpor response and reduced Beta adrenergic receptor expression in adipose tissue.
      Authors: Wankhade UD, Vella KR, Fox DL
      PLoS ONE, 2010;5(8):e12324.
      Species: Mouse
      Sample Type: Serum
    33. Group X secretory phospholipase A2 negatively regulates adipogenesis in murine models.
      Authors: Li X, Shridas P, Forrest K, Bailey W, Webb NR
      FASEB J., 2010;24(11):4313-24.
      Species: Mouse
      Sample Type: Plasma
    34. Moderate caloric restriction in lactating rats protects offspring against obesity and insulin resistance in later life.
      Authors: Palou M, Priego T, Sanchez J, Torrens JM, Palou A, Pico C
      Endocrinology, 2010;151(3):1030-41.
      Species: Rat
      Sample Type: Milk
    35. Differential effects of high MUFA with high or low P/S ratio (polyunsaturated to saturated fatty acids) on improving hepatic lipolytic enzymes and mediating PPARgamma related with lipoprotein lipase and hormone-sensitive lipase of white adipose tissue in diet-induced obese hamster.
      Authors: Liao FH, Liou TH, Chiu WC, Shieh MJ, Chien YW
      Int J Obes (Lond), 2010;34(11):1608-17.
      Species: Hamster
      Sample Type: Plasma
    36. Efficient method for obtaining Lep(ob)/Lep(ob)-derived animal models using adipose tissue transplantations.
      Authors: Barros CC, Almeida SS, Mori MA, Valero VB, Haro AS, Batista EC, Rosa TS, Bacurau RF, Wurtele M, Araujo RC
      Int J Obes (Lond), 2009;33(8):938-44.
      Species: Mouse
      Sample Type: Serum
    37. Early handling increases susceptibility to experimental autoimmune encephalomyelitis (EAE) in C57BL/6 male mice.
      Authors: Columba-Cabezas S, Iaffaldano G, Chiarotti F, Alleva E, Cirulli F
      J. Neuroimmunol., 2009;212(1):10-6.
      Species: Mouse
      Sample Type: Serum
    38. Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
      Authors: Lim G, Wang S, Zhang Y, Tian Y, Mao J
      J. Clin. Invest., 2009;119(2):295-304.
      Species: Rat
      Sample Type: Plasma
    39. Development of obesity in the Otsuka Long-Evans Tokushima Fatty rat.
      Authors: Schroeder M, Zagoory-Sharon O, Shbiro L, Marco A, Hyun J, Moran TH, Bi S, Weller A
      Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009;297(6):R1749-60.
      Species: Rat
      Sample Type: Plasma
    40. Stearoyl-CoA desaturase 1 deficiency protects mice from immune-mediated liver injury.
      Authors: Feng D, Wang Y, Mei Y, Xu Y, Xu H, Lu Y, Luo Q, Zhou S, Kong X, Xu L
      Lab. Invest., 2009;89(2):222-30.
      Species: Mouse
      Sample Type: Plasma
    41. Hepatic CB1 receptor is required for development of diet-induced steatosis, dyslipidemia, and insulin and leptin resistance in mice.
      Authors: Osei-Hyiaman D, Liu J, Zhou L, Godlewski G, Harvey-White J, Jeong WI, Batkai S, Marsicano G, Lutz B, Buettner C, Kunos G
      J. Clin. Invest., 2008;118(9):3160-9.
      Species: Mouse
      Sample Type: Plasma
    42. Octylphenol stimulates resistin gene expression in 3T3-L1 adipocytes via the estrogen receptor and extracellular signal-regulated kinase pathways.
      Authors: Lee MJ, Lin H, Liu CW, Wu MH, Liao WJ, Chang HH, Ku HC, Chien YS, Ding WH, Kao YH
      Am. J. Physiol., Cell Physiol., 2008;294(6):C1542-51.
      Species: Mouse
      Sample Type: Serum
    43. Doubly truncated FosB isoform (Delta2DeltaFosB) induces osteosclerosis in transgenic mice and modulates expression and phosphorylation of Smads in osteoblasts independent of intrinsic AP-1 activity.
      Authors: Sabatakos G, Rowe GC, Kveiborg M, Wu M, Neff L, Chiusaroli R, Philbrick WM, Baron R
      J. Bone Miner. Res., 2008;23(5):584-95.
      Species: Mouse
      Sample Type: Serum
    44. Identification of ALOX5 as a gene regulating adiposity and pancreatic function.
      Authors: Mehrabian M, Schulthess FT, Nebohacova M, Castellani LW, Zhou Z, Hartiala J, Oberholzer J, Lusis AJ, Maedler K, Allayee H
      Diabetologia, 2008;51(6):978-88.
      Species: Mouse
      Sample Type: Plasma
    45. Signal transducer and activator of transcription-3 is required in hypothalamic agouti-related protein/neuropeptide Y neurons for normal energy homeostasis.
      Authors: Gong L, Yao F, Hockman K, Heng HH, Morton GJ, Takeda K, Akira S, Low MJ, Rubinstein M, MacKenzie RG
      Endocrinology, 2008;149(7):3346-54.
      Species: Mouse
      Sample Type: Plasma
    46. Adaptation to lactation in OLETF rats lacking CCK-1 receptors: body weight, fat tissues, leptin and oxytocin.
      Authors: Zagoory-Sharon O, Schroeder M, Levine A, Moran TH, Weller A
      Int J Obes (Lond), 2008;32(8):1211-21.
      Species: Rat
      Sample Type: Plasma
    47. Novel black soy peptides with antiobesity effects: activation of leptin-like signaling and AMP-activated protein kinase.
      Authors: Jang EH, Moon JS, Ko JH, Ahn CW, Lee HH, Shin JK, Park CS, Kang JH
      Int J Obes (Lond), 2008;32(7):1161-70.
      Species: Mouse
      Sample Type: Plasma
    48. Role and regulation of adipokines during zymosan-induced peritoneal inflammation in mice.
      Authors: Pini M, Gove ME, Sennello JA, van Baal JW, Chan L, Fantuzzi G
      Endocrinology, 2008;149(8):4080-5.
      Species: Mouse
      Sample Type: Serum
    49. Leptin induces elongation of cardiac myocytes and causes eccentric left ventricular dilatation with compensation.
      Authors: Abe Y, Ono K, Kawamura T, Wada H, Kita T, Shimatsu A, Hasegawa K
      Am. J. Physiol. Heart Circ. Physiol., 2007;292(5):H2387-96.
      Species: Mouse
      Sample Type: Plasma
    50. High circulating leptin receptors with normal leptin sensitivity in liver-specific insulin receptor knock-out (LIRKO) mice.
      Authors: Cohen SE, Kokkotou E, Biddinger SB, Kondo T, Gebhardt R, Kratzsch J, Mantzoros CS, Kahn CR
      J. Biol. Chem., 2007;282(32):23672-8.
      Species: Mouse
      Sample Type: Serum
    51. FXR-deficiency confers increased susceptibility to torpor.
      Authors: Cariou B, Bouchaert E, Abdelkarim M, Dumont J, Caron S, Fruchart JC, Burcelin R, Kuipers F, Staels B
      FEBS Lett., 2007;581(27):5191-8.
      Species: Mouse
      Sample Type: Plasma
    52. Long-term blockade of angiotensin AT1 receptors increases survival of obese Zucker rats.
      Authors: O&amp;amp;apos;Connor SE
      Eur. J. Pharmacol., 2006;534(1):271-9.
      Species: Mouse
      Sample Type: Plasma
    53. Leptin resistance protects mice from hyperoxia-induced acute lung injury.
      Authors: Bellmeyer A, Martino JM, Chandel NS, Scott Budinger GR, Dean DA, Mutlu GM
      Am. J. Respir. Crit. Care Med., 2006;175(6):587-94.
      Species: Mouse
      Sample Type: BALF
    54. Impaired adipose tissue development in mice with inactivation of placental growth factor function.
      Authors: Lijnen HR, Christiaens V, Scroyen I, Voros G, Tjwa M, Carmeliet P, Collen D
      Diabetes, 2006;55(10):2698-704.
      Species: Mouse
      Sample Type: Tissue Homogenates
    55. Metformin restores leptin sensitivity in high-fat-fed obese rats with leptin resistance.
      Authors: Kim YW, Kim JY, Park YH, Park SY, Won KC, Choi KH, Huh JY, Moon KH
      Diabetes, 2006;55(3):716-24.
      Species: Rat
      Sample Type: CSF
    56. Integrating genetic and network analysis to characterize genes related to mouse weight.
      Authors: Ghazalpour A, Doss S, Zhang B, Wang S, Plaisier C, Castellanos R, Brozell A, Schadt EE, Drake TA, Lusis AJ, Horvath S
      PLoS Genet., 2006;2(8):e130.
      Species: Mouse
      Sample Type: Plasma
    57. Role of leptin deficiency in early acute renal failure during endotoxemia in ob/ob mice.
      Authors: Wang W, Poole B, Mitra A, Falk S, Fantuzzi G, Lucia S, Schrier R
      J. Am. Soc. Nephrol., 2004;15(3):645-9.
      Species: Mouse
      Sample Type: Serum
    58. Diurnal rhythms of leptin and ghrelin in the systemic circulation and in the gastric mucosa are related to food intake in rats.
      Authors: Sanchez J, Oliver P, Pico C, Palou A
      Pflugers Arch., 2004;448(5):500-6.
      Species: Rat
      Sample Type: Serum
    59. The increased bone mass in deltaFosB transgenic mice is independent of circulating leptin levels.
      Authors: Kveiborg M, Chiusaroli R, Sims NA, Wu M, Sabatakos G, Horne WC, Baron R
      Endocrinology, 2002;143(11):4304-9.
      Species: Mouse
      Sample Type: Serum
    60. Leptin accelerates autoimmune diabetes in female NOD mice.
      Authors: Matarese G, Sanna V, Lechler RI, Sarvetnick N, Fontana S, Zappacosta S, La Cava A
      Diabetes, 2002;51(5):1356-61.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    61. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity.
      Authors: Yamauchi, T, Kamon, J, Waki, H, Terauchi, Y, Kubota, N, Hara, K, Mori, Y, Ide, T, Murakami, K, Tsuboyama-Kasaoka, N, Ezaki, O, Akanuma, Y, Gavrilova, O, Vinson, C, Reitman, M L, Kagechika, H, Shudo, K, Yoda, M, Nakano, Y, Tobe, K, Nagai, R, Kimura, S, Tomita, M, Froguel, P, Kadowaki, T
      Nat Med, 2001;7(8):941-6.
      Species: Mouse
      Sample Type: Plasma
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    1. Why is the acid activation procedure required for pregnant mouse serum samples from second trimester through post-partum day 1 in the Mouse/Rat Leptin Quantikine® ELISA assay?

      • Mouse Leptin R intereferes in the MOB00 assay at high concentration >2000 pg/mL, and studies have shown that there is a massive increase in the level of criculating Leptin Receptor (OB-R) during later stage of pregnancy. Acid activation helps to dissociate the interaction between Leptin and its high affinity recceptor OB-R thus minimizing possible interference in the Leptin assay. Note that activation is not required for samples obtained from non-pregnant mice. Ref. J. Biol. Chem (1997) 272(48):30546-51.

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