Detects mouse and rat M‑Cadherin/Cadherin‑15 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human M‑Cadherin/Cadherin‑15 is observed, and less than 1% cross-reactivity with recombinant mouse P-Cadherin is observed.
Detection of Mouse and Rat M‑Cadherin/ Cadherin‑15 by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line, mouse brain (cerebellum) tissue, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat M‑Cadherin/Cadherin‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7677) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for M‑Cadherin/Cadherin‑15 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CDH-15 (Cadherin 15; also M-cadherin, Muscle cadherin and Cadherin-14) is a 125-127 kDa atypical member of the classical cadherin family, cadherin superfamily of molecules. It is expressed by muscle satellite cells, cells of the embryonic myotome, and hematopoietic bone marrow stem cells. CDH-15 appears to bind homotypically in trans, thus allowing for the identification and subsequent fusion of myoblast precursors, particularly those in slow-twitch (or red fiber) muscles. This is accompanied by a downregulation of mitochondrial induced apoptosis. Mouse CDH-15 is synthesized as a 784 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, a 38 aa propeptide, and a 725 aa mature region. The mature region is expressed as a type I transmembrane glycoprotein that possesses a 546 aa extracellular region (aa 60-605) and a 159 aa cytoplasmic domain (aa 626-784). The extracellular region shows five consecutive cadherin domains. Over aa 22-605, mouse CDH-15 shares 88% and 97% aa sequence identity with human and rat CDH-15, respectively.
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