Mouse/Rat M-Cadherin/Cadherin-15 Antibody

Catalog # Availability Size / Price Qty
AF7677
AF7677-SP
Detection of Mouse and Rat M‑Cadherin/ Cadherin‑15 by Western Blot.
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Product Details
Citations (1)
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Mouse/Rat M-Cadherin/Cadherin-15 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat M‑Cadherin/Cadherin‑15  in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human M‑Cadherin/Cadherin‑15 is observed, and less than 1% cross-reactivity with recombinant mouse P-Cadherin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse M‑Cadherin/Cadherin‑15
Val22-Ala605 (Arg159His)
Accession # P33146
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat M-Cadherin/ Cadherin-15 antibody by Western Blot. View Larger

Detection of Mouse and Rat M‑Cadherin/ Cadherin‑15 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, mouse brain (cerebellum) tissue, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat M-Cadherin/Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7677) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for M-Cadherin/Cadherin-15 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry M-Cadherin/Cadherin-15 antibody in Human Embryonic Muscle by Immunohistochemistry (IHC-Fr). View Larger

M‑Cadherin/Cadherin‑15 in Human Embryonic Muscle. M-Cadherin/Cadherin-15 was detected in perfusion fixed frozen sections of human embryonic muscle using Sheep Anti-Mouse/Rat M-Cadherin/Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7677) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-Cadherin/Cadherin-15

CDH-15 (Cadherin 15; also M-cadherin, Muscle cadherin and Cadherin-14) is a 125-127 kDa atypical member of the classical cadherin family, cadherin superfamily of molecules. It is expressed by muscle satellite cells, cells of the embryonic myotome, and hematopoietic bone marrow stem cells. CDH-15 appears to bind homotypically in trans, thus allowing for the identification and subsequent fusion of myoblast precursors, particularly those in slow-twitch (or red fiber) muscles. This is accompanied by a downregulation of mitochondrial induced apoptosis. Mouse CDH-15 is synthesized as a 784 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, a 38 aa propeptide, and a 725 aa mature region. The mature region is expressed as a type I transmembrane glycoprotein that possesses a 546 aa extracellular region (aa 60-605) and a 159 aa cytoplasmic domain (aa 626-784). The extracellular region shows five consecutive cadherin domains. Over aa 22-605, mouse CDH-15 shares 88% and 97% aa sequence identity with human and rat CDH-15, respectively.

Long Name
M-Cadherin [Myotubule]
Entrez Gene IDs
1013 (Human); 12555 (Mouse); 361432 (Rat)
Alternate Names
cadherin 15, M-cadherin (myotubule); cadherin 15, type 1, M-cadherin (myotubule); Cadherin-14; Cadherin-15; cadherin-3; CDH14; CDH14cadherin-14; CDH15; CDH3cadherin-15; CDHM; MCAD; MCadherin; M-Cadherin; MRD3; Muscle cadherin; muscle-cadherin

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Citation for Mouse/Rat M-Cadherin/Cadherin-15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Impairment of cold injury-induced muscle regeneration in mice receiving a combination of bone fracture and alendronate treatment
    Authors: S Kawada, A Harada, N Hashimoto
    PLoS ONE, 2017-07-17;12(7):e0181457.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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