Mouse S100A10 Antibody

Catalog # Availability Size / Price Qty
MAB2377
MAB2377-SP
Mouse S100A10 Antibody in Data
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Product Details
Citations (2)
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Mouse S100A10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S100A10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A9, recombinant human (rh) S100B, or rhS100P is observed.
Source
Monoclonal Rat IgG2A Clone # 344015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse S100A10
Met1-Lys97
Accession # P08207
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
TK‑1 mouse T cell lymphoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
Immersion fixed TK-1 mouse lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse S100A10 antibody by Western Blot. View Larger

Detection of Mouse S100A10 by Western Blot. Western blot shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A10

S100A10, also called p11, is a 97 amino acid member of the S100 family of EF hand proteins, but does not bind calcium. It is primarily found, either within or on the surface of mast cells, as a complex of two annexin A2 subunits with two S100A10 subunits. Extracellular S100A10 is a plasminogen receptor important for plasmin production and cellular invasiveness. Intracellular S100A10 may target ligands to the endoplasmic reticulum. Mouse S100A10 shows 92% amino acid identity with human S100A10.

Long Name
S100 Calcium Binding Protein
Entrez Gene IDs
6281 (Human); 20194 (Mouse)
Alternate Names
annexin II ligand, calpactin I, light polypeptide; ANX2L; ANX2LG; CAL1LGP11,42C; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; CLP11Ca[1]; MGC111133; p10 protein; p10; P11; protein S100-A10; S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium binding protein A10; S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium-binding protein A10; S100A10

Product Datasheets

Citations for Mouse S100A10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. PSA-NCAM colocalized with cholecystokinin-expressing cells in the hippocampus is involved in mediating antidepressant efficacy
    Authors: J Yamada, C Sato, K Konno, M Watanabe, S Jinno
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Annexin2 coating the surface of enlargeosomes is needed for their regulated exocytosis.
    Authors: Lorusso A, Covino C, Priori G, Bachi A, Meldolesi J, Chieregatti E
    EMBO J., 2006;25(23):5443-56.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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Secondary Antibodies

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