Mouse S100A10 Antibody

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Detection of Mouse S100A10 by Western Blot.
3 Images
Product Details
Citations (2)
Supplemental Products

Mouse S100A10 Antibody Summary

Species Reactivity
Detects mouse S100A10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A9, recombinant human (rh) S100B, or rhS100P is observed.
Monoclonal Rat IgG2A Clone # 344015
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse S100A10
Accession # P08207
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
Raw264 mouse monocyte/macrophage cell line or TK‑1 mouse T cell lymphoma cell line
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
5-25 µg/mL
immersion fixed TK‑1 mouse T cell lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse S100A10 antibody by Western Blot. View Larger

Detection of Mouse S100A10 by Western Blot. Western blot shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry View Larger

S100A10 in TK‑1 Mouse T Cell Line. S100A10 was detected in immersion fixed TK‑1 mouse T cell lymphoma cell line using Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry View Larger

Detection of S100A10 in Raw264 Mouse Cell Line by Flow Cytometry. Raw264 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377, filled histogram) or Rat IgG2A Isotype Control (MAB006, open histogram) followed by PE-conjugated Anti-Rat IgG Secondary Antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). Staining was performed using our Staining Intracellular Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A10

S100A10, also called p11, is a 97 amino acid member of the S100 family of EF hand proteins, but does not bind calcium. It is primarily found, either within or on the surface of mast cells, as a complex of two annexin A2 subunits with two S100A10 subunits. Extracellular S100A10 is a plasminogen receptor important for plasmin production and cellular invasiveness. Intracellular S100A10 may target ligands to the endoplasmic reticulum. Mouse S100A10 shows 92% amino acid identity with human S100A10.

Long Name
S100 Calcium Binding Protein
Entrez Gene IDs
6281 (Human); 20194 (Mouse)
Alternate Names
annexin II ligand, calpactin I, light polypeptide; ANX2L; ANX2LG; CAL1LGP11,42C; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; CLP11Ca[1]; MGC111133; p10 protein; p10; P11; protein S100-A10; S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium binding protein A10; S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium-binding protein A10; S100A10

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Citations for Mouse S100A10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. PSA-NCAM colocalized with cholecystokinin-expressing cells in the hippocampus is involved in mediating antidepressant efficacy
    Authors: J Yamada, C Sato, K Konno, M Watanabe, S Jinno
    J. Neurosci., 2019-12-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Annexin2 coating the surface of enlargeosomes is needed for their regulated exocytosis.
    Authors: Lorusso A, Covino C, Priori G, Bachi A, Meldolesi J, Chieregatti E
    EMBO J., 2006-11-02;25(23):5443-56.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC


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