Detects mouse S100A10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A9, recombinant human (rh) S100B, or rhS100P is observed.
Monoclonal Rat IgG2A Clone # 344015
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse S100A10 Met1-Lys97 Accession # P08207
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
TK‑1 mouse T cell lymphoma cell line
Immersion fixed TK-1 mouse lymphoma cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse S100A10 by Western Blot. Western blot shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
S100A10, also called p11, is a 97 amino acid member of the S100 family
of EF hand proteins, but does not bind calcium. It is primarily found,
either within or on the surface of mast cells, as a complex of two
annexin A2 subunits with two S100A10 subunits. Extracellular S100A10 is
a plasminogen receptor important for plasmin production and cellular
invasiveness. Intracellular S100A10 may target ligands to the
endoplasmic reticulum. Mouse S100A10 shows 92% amino acid identity with
S100 Calcium Binding Protein
Entrez Gene IDs:
6281 (Human); 20194 (Mouse)
annexin II ligand, calpactin I, light polypeptide; ANX2L; ANX2LG; CAL1LGP11,42C; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; CLP11Ca; MGC111133; p10 protein; p10; P11; protein S100-A10; S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium binding protein A10; S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium-binding protein A10
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.