Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 344015
View all S100A10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse S100A10
Met1-Lys97
Accession # P08207

Specificity

Detects mouse S100A10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A9, recombinant human (rh) S100B, or rhS100P is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse S100A10 Antibody

Detection of Mouse S100A10 antibody by Western Blot.

Detection of Mouse S100A10 by Western Blot.

Western blot shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

S100A10 in TK‑1 Mouse T Cell Line.

S100A10 was detected in immersion fixed TK‑1 mouse T cell lymphoma cell line using Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of S100A10 in Raw264 Mouse Cell Line by Flow Cytometry.

Raw264 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377, filled histogram) or Rat IgG2A Isotype Control (MAB006, open histogram) followed by PE-conjugated Anti-Rat IgG Secondary Antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). Staining was performed using our Staining Intracellular Proteins protocol.

Applications for Mouse S100A10 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Raw264 mouse monocyte/macrophage cell line or TK‑1 mouse T cell lymphoma cell line

Immunocytochemistry

5-25 µg/mL
Sample: immersion fixed TK‑1 mouse T cell lymphoma cell line

Western Blot

1 µg/mL
Sample: Mouse lung tissue and MEF mouse embryonic feeder cells

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A10

S100A10, also called p11, is a 97 amino acid member of the S100 family of EF hand proteins, but does not bind calcium. It is primarily found, either within or on the surface of mast cells, as a complex of two annexin A2 subunits with two S100A10 subunits. Extracellular S100A10 is a plasminogen receptor important for plasmin production and cellular invasiveness. Intracellular S100A10 may target ligands to the endoplasmic reticulum. Mouse S100A10 shows 92% amino acid identity with human S100A10.

Long Name

S100 Calcium Binding Protein

Alternate Names

annexin II ligand, calpactin I, light polypeptide, ANX2L, ANX2LG, CAL1LGP11,42C, Calpactin I light chain, Calpactin-1 light chain, Cellular ligand of annexin II, CLP11Ca[1], MGC111133, p10, p10 protein, p11, protein S100-A10, S100 calcium binding protein A10, S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)), S100 calcium-binding protein A10, S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11))

Entrez Gene IDs

6281 (Human); 20194 (Mouse)

Gene Symbol

S100A10

UniProt

P08207

Additional S100A10 Products

Product Documents for Mouse S100A10 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse S100A10 Antibody

For research use only

Citations for Mouse S100A10 Antibody

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Protocols

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