Mouse S100A8 DuoSet ELISA

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Ancillary Products Available
Mouse S100A8 ELISA Standard Curve
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Product Details
Citations (11)
Supplemental Products
Reviews (2)

Mouse S100A8 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse S100A8. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Mouse S100A8 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100A8

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Long Name:
S100 Calcium Binding Protein A8
Entrez Gene IDs:
6279 (Human); 20201 (Mouse)
Alternate Names:
60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A

Citations for Mouse S100A8 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Pro-inflammatory megakaryocyte gene expression in murine models of breast cancer
    Authors: HG Roweth, MW Malloy, GJ Goreczny, IC Becker, Q Guo, EA Mittendorf, JE Italiano, SS McAllister, EM Battinelli
    Science Advances, 2022-10-12;8(41):eabo5224.
    Species: Mouse
    Sample Types: Plasma
  2. Psychological stress impairs IL22-driven protective gut mucosal immunity against colonising pathobionts
    Authors: CR Shaler, AA Parco, W Elhenawy, J Dourka, J Jury, EF Verdu, BK Coombes
    Nature Communications, 2021-11-18;12(1):6664.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. alpha-galactosylceramide-stimulated invariant natural killer T-cells play a protective role in murine vulvovaginal candidiasis by Candida albicans
    Authors: M Abe, Y Kinjo, S Sadamoto, M Shinozaki, M Nagi, K Shibuya, Y Miyazaki
    PLoS ONE, 2021-11-16;16(11):e0259306.
    Species: Mouse
    Sample Types: Vaginal Lavage
  4. Severity of Experimental Autoimmune Uveitis Is Reduced by Pretreatment with Live Probiotic Escherichia coli Nissle 1917
    Authors: O Dusek, A Fajstova, A Klimova, P Svozilkova, T Hrncir, M Kverka, S Coufal, J Slemin, H Tlaskalova, JV Forrester, J Heissigero
    Cells, 2020-12-25;10(1):.
    Species: Mouse
    Sample Types: Tissue Culture Supernates
  5. Biotin Supplementation Ameliorates Murine Colitis by Preventing NF-kappaB Activation: Biotin supplementation ameliorates colitis
    Authors: J Skupsky, S Sabui, M Hwang, M Nakasaki, MD Cahalan, HM Said
    Cell Mol Gastroenterol Hepatol, 2019-11-29;0(0):.
    Species: Mouse
    Sample Types: Feces
  6. Intestinal Epithelial Cells and the Microbiome Undergo Swift Reprogramming at the Inception of Colonic Citrobacter rodentium Infection
    Authors: EGD Hopkins, TI Roumelioti, C Mullineaux, JS Choudhary, G Frankel
    MBio, 2019-04-02;10(2):.
    Species: Mouse
    Sample Types: Feces
  7. Exacerbation of hepatic injury during rodent malaria by myeloid-related protein 14
    Authors: H Mizobuchi, W Fujii, S Isokawa, K Ishizuka, Y Wang, S Watanabe, C Sanjoba, Y Matsumoto, Y Goto
    PLoS ONE, 2018-06-14;13(6):e0199111.
    Species: Mouse
    Sample Types: Serum
  8. Visualization of Tumor-Immune Interaction - Target-Specific Imaging of S100A8/A9 Reveals Pre-Metastatic Niche Establishment
    Authors: M Eisenblaet, F Flores-Bor, JJ Lee, C Wefers, H Smith, R Hueting, MS Cooper, PJ Blower, D Patel, M Rodriguez-, H Milewicz, T Vogl, J Roth, A Tutt, T Schaeffter, T Ng
    Theranostics, 2017-06-15;7(9):2392-2401.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Vaginal Heparan Sulfate Linked to Neutrophil Dysfunction in the Acute Inflammatory Response Associated with Experimental Vulvovaginal Candidiasis
    Authors: J Yano, MC Noverr, PL Fidel
    MBio, 2017-03-14;8(2):.
    Species: Mouse
    Sample Types: Vaginal Lavage Fluid
  10. A Murine Model of Candida glabrata Vaginitis Shows No Evidence of an Inflammatory Immunopathogenic Response.
    Authors: Nash E, Peters B, Lilly E, Noverr M, Fidel P
    PLoS ONE, 2016-01-25;11(1):e0147969.
    Species: Mouse
    Sample Types: Vaginal Lavage Fluid
  11. Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner.
    Authors: Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R
    Infect Immun, 2014-06-23;82(9):3723-39.
    Species: Mouse
    Sample Types: Tissue Homogenates


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Reviews for Mouse S100A8 DuoSet ELISA

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Mouse S100A8 DuoSet ELISA
By Anonymous on 03/11/2022
Sample Tested: Serum and Plasma

Used this kit for quantifying mouse S100A8 in serum samples but did not see any detecting in our samples. Standard curve looks good.
***Bio-Techne Response: We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
DuoSet kits are validated for cell culture supernatants.
Working with other type of samples may require some empirical validation because factors like sample matrix etc. can impact antibody binding.
We recommend to perform Spike, Recovery, and Linearity tests to optimize the conditions for any other sample types.***

Mouse S100A8 DuoSet ELISA
By Anonymous on 08/03/2016
Sample Tested: Mouse BAL fluid