Mouse S100A8 DuoSet ELISA

R&D Systems | Catalog # DY3059

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

31.2-2000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse S100A8 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all S100A8 ELISA Kits »

Product Summary for Mouse S100A8 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse S100A8. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Mouse S100A8 DuoSet ELISA

Mouse S100A8 ELISA Standard Curve

Mouse S100A8 ELISA Standard Curve

Kit Contents for Mouse S100A8 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS:

(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: S100A8

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Entrez Gene IDs

6279 (Human); 20201 (Mouse)

Gene Symbol

S100A8

Additional S100A8 Products

Product Documents for Mouse S100A8 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse S100A8 DuoSet ELISA

For research use only

Citations for Mouse S100A8 DuoSet ELISA

Customer Reviews for Mouse S100A8 DuoSet ELISA (2)

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  • Mouse S100A8 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 03/11/2022
    Used this kit for quantifying mouse S100A8 in serum samples but did not see any detecting in our samples. Standard curve looks good.
    Mouse S100A8 DuoSet ELISA DY3059
    Bio-Techne Response
    We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. DuoSet kits are validated for cell culture supernatants. Working with other type of samples may require some empirical validation because factors like sample matrix etc. can impact antibody binding. We recommend to perform Spike, Recovery, and Linearity tests to optimize the conditions for any other sample types.
  • Mouse S100A8 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Mouse BAL fluid
    Verified Customer | Posted 08/03/2016
    Mouse S100A8 DuoSet ELISA DY3059

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